Calreticulina Canina (Canis lupus familiaris) en Procesos Experimentales de Cicatrización. Comparación Con Calreticulinas Humana y de Trypanosoma cruzi.
Calreticulin (CRT) is an endoplasmic reticulum (ER) chaperone protein, with many functions such as calcium storage, proper protein folding as well as antiangiogenic and antitumor properties. A wound-healing role for CRT from both human and parasitic origins has been described. However, this function...
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ftconicyt:oai:localhost:10533/219259 2023-05-15T15:49:40+02:00 Calreticulina Canina (Canis lupus familiaris) en Procesos Experimentales de Cicatrización. Comparación Con Calreticulinas Humana y de Trypanosoma cruzi. Calreticulina canina (canis lupus familiaris) en procesos experimentales de cicatrización. Comparación con calreticulinas humana y de trypanosoma cruzi. Rosas-Chuñil, Carlos Alberto Ferreira-Vigouroux, Luis Arturo UNIVERSIDAD DE CHILE Chile http://hdl.handle.net/10533/219259 unknown instname: Conicyt reponame: Repositorio Digital RI2.0 info:eu-repo/grantAgreement//1130099 info:eu-repo/semantics/dataset/hdl.handle.net/10533/93488 http://hdl.handle.net/10533/219259 info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ CC-BY-NC-ND Medicina y Ciencias de la Salud info:eu-repo/semantics/doctoralThesis ftconicyt 2019-09-07T08:29:08Z Calreticulin (CRT) is an endoplasmic reticulum (ER) chaperone protein, with many functions such as calcium storage, proper protein folding as well as antiangiogenic and antitumor properties. A wound-healing role for CRT from both human and parasitic origins has been described. However, this function has not been described in species frequently exposed to wounds, such as the domestic dog (Canis lupus familiaris) and its ancestors, the wolves (C. lupus). Therefore, in this Doctoral Thesis we hypothesize that CRT from C. lupus familiaris (CfCRT) accelerates wound-healing processes in in vivo assays. Native CfCRT was obtained from canine placentas, to be used in a variety of in vivo assays. Furthermore, it was also used in an in vivo wound-healing model in rats (Rattus norvegicus), where CfCRT, human CRT (HuCRT), Trypanosoma cruzi CRT (TcCRT), canine serum albumin (CASA), Platelet-derived growth factor (PDGF) or PBS were applied. The results show that: i) Native CfCRT (co-purified with CASA), was obtained, by a combination of chromatographic criteria, reinforced by antigenic and functional approaches, together with Mass Spectrometry; ii). Independent of its source, CRT promotes wound healing, although TcCRT and CfCRT are more effective; iii). The TcCRT effect on wound healing could be mediated, at least in part, by an increment in TGF-β3 expression; iv). An increment in cell proliferation and collagen fibers was evident in tissue granulation. These results agree with previous reports where CRT promotes wound healing. Our comparative results establish different degrees of effectiveness among different CRT’s. As projection of these results, the identification of CfCRT and / or TcCRT domains responsible for the positive modulation of the wound healing effect. Doctoral or Postdoctoral Thesis Canis lupus Repositorio Digital Conicyt RI 2.0 (Comisión Nacional de Investigación Científica y Tecnológica) |
institution |
Open Polar |
collection |
Repositorio Digital Conicyt RI 2.0 (Comisión Nacional de Investigación Científica y Tecnológica) |
op_collection_id |
ftconicyt |
language |
unknown |
topic |
Medicina y Ciencias de la Salud |
spellingShingle |
Medicina y Ciencias de la Salud Rosas-Chuñil, Carlos Alberto Calreticulina Canina (Canis lupus familiaris) en Procesos Experimentales de Cicatrización. Comparación Con Calreticulinas Humana y de Trypanosoma cruzi. |
topic_facet |
Medicina y Ciencias de la Salud |
description |
Calreticulin (CRT) is an endoplasmic reticulum (ER) chaperone protein, with many functions such as calcium storage, proper protein folding as well as antiangiogenic and antitumor properties. A wound-healing role for CRT from both human and parasitic origins has been described. However, this function has not been described in species frequently exposed to wounds, such as the domestic dog (Canis lupus familiaris) and its ancestors, the wolves (C. lupus). Therefore, in this Doctoral Thesis we hypothesize that CRT from C. lupus familiaris (CfCRT) accelerates wound-healing processes in in vivo assays. Native CfCRT was obtained from canine placentas, to be used in a variety of in vivo assays. Furthermore, it was also used in an in vivo wound-healing model in rats (Rattus norvegicus), where CfCRT, human CRT (HuCRT), Trypanosoma cruzi CRT (TcCRT), canine serum albumin (CASA), Platelet-derived growth factor (PDGF) or PBS were applied. The results show that: i) Native CfCRT (co-purified with CASA), was obtained, by a combination of chromatographic criteria, reinforced by antigenic and functional approaches, together with Mass Spectrometry; ii). Independent of its source, CRT promotes wound healing, although TcCRT and CfCRT are more effective; iii). The TcCRT effect on wound healing could be mediated, at least in part, by an increment in TGF-β3 expression; iv). An increment in cell proliferation and collagen fibers was evident in tissue granulation. These results agree with previous reports where CRT promotes wound healing. Our comparative results establish different degrees of effectiveness among different CRT’s. As projection of these results, the identification of CfCRT and / or TcCRT domains responsible for the positive modulation of the wound healing effect. |
author2 |
Ferreira-Vigouroux, Luis Arturo UNIVERSIDAD DE CHILE |
format |
Doctoral or Postdoctoral Thesis |
author |
Rosas-Chuñil, Carlos Alberto |
author_facet |
Rosas-Chuñil, Carlos Alberto |
author_sort |
Rosas-Chuñil, Carlos Alberto |
title |
Calreticulina Canina (Canis lupus familiaris) en Procesos Experimentales de Cicatrización. Comparación Con Calreticulinas Humana y de Trypanosoma cruzi. |
title_short |
Calreticulina Canina (Canis lupus familiaris) en Procesos Experimentales de Cicatrización. Comparación Con Calreticulinas Humana y de Trypanosoma cruzi. |
title_full |
Calreticulina Canina (Canis lupus familiaris) en Procesos Experimentales de Cicatrización. Comparación Con Calreticulinas Humana y de Trypanosoma cruzi. |
title_fullStr |
Calreticulina Canina (Canis lupus familiaris) en Procesos Experimentales de Cicatrización. Comparación Con Calreticulinas Humana y de Trypanosoma cruzi. |
title_full_unstemmed |
Calreticulina Canina (Canis lupus familiaris) en Procesos Experimentales de Cicatrización. Comparación Con Calreticulinas Humana y de Trypanosoma cruzi. |
title_sort |
calreticulina canina (canis lupus familiaris) en procesos experimentales de cicatrización. comparación con calreticulinas humana y de trypanosoma cruzi. |
url |
http://hdl.handle.net/10533/219259 |
op_coverage |
Chile |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
instname: Conicyt reponame: Repositorio Digital RI2.0 info:eu-repo/grantAgreement//1130099 info:eu-repo/semantics/dataset/hdl.handle.net/10533/93488 http://hdl.handle.net/10533/219259 |
op_rights |
info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivs 3.0 Chile http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ |
op_rightsnorm |
CC-BY-NC-ND |
_version_ |
1766384701842391040 |