Polyclonal antibodies anti-salmon immunoglobulins; potential tools for diagnosis

Immunoglobulins (Igs) from sera of Atlantic salmon (Salmo salar) were purified with 50% saturated ammonium sulphate followed by molecular sieving chromatography using Sephacryl S-300. The fraction of purified Igs was used as immunogen to generate a polyclonal antibody anti-salmon Tgs. This antibody...

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Main Authors: Ferreira-Vigouroux, Luis, Aguillón-Gutiérrez, Juan, Molina-Sampayo, María, Colombo, Alicia, Smith, Pedro
Format: Article in Journal/Newspaper
Language:Spanish
Published: UNIV AUSTRAL CHILE, FAC CIENCIAS VETERINARIAS
Subjects:
Atlantic salmon
Salmo salar
Online Access:http://hdl.handle.net/10533/197442
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record_format openpolar
spelling ftconicyt:oai:localhost:10533/197442 2023-05-15T15:31:36+02:00 Polyclonal antibodies anti-salmon immunoglobulins; potential tools for diagnosis Ferreira-Vigouroux, Luis Aguillón-Gutiérrez, Juan Molina-Sampayo, María Colombo, Alicia Smith, Pedro CHL VALDIVIA http://hdl.handle.net/10533/197442 doi:0 SPA spa UNIV AUSTRAL CHILE, FAC CIENCIAS VETERINARIAS instname: Conicyt reponame: Repositorio Digital RI2.0 info:eu-repo/grantAgreement/Fondef/D96T1023 info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477 http://hdl.handle.net/10533/197442 doi:0 wos: WOS:A1997XJ66200016 eissn: 0 issn: 0301-732X info:eu-repo/semantics/openAccess ARCHIVOS DE MEDICINA VETERINARIA info:eu-repo/semantics/article ftconicyt 2019-09-07T08:18:24Z Immunoglobulins (Igs) from sera of Atlantic salmon (Salmo salar) were purified with 50% saturated ammonium sulphate followed by molecular sieving chromatography using Sephacryl S-300. The fraction of purified Igs was used as immunogen to generate a polyclonal antibody anti-salmon Tgs. This antibody was affinity purified in a Sepharose-protein G column. By immunoradiometric assay against the immunogen, the rabbit IgG anti-salmon Igs showed a high specific titer (at least 1/100.000) for the antigen. This reagent offers the possibility of detection of salmon or trout (crossreactivity) humoral immune responses, both for diagnostic and research purposes. Article in Journal/Newspaper Atlantic salmon Salmo salar Repositorio Digital Conicyt RI 2.0 (Comisión Nacional de Investigación Científica y Tecnológica)
institution Open Polar
collection Repositorio Digital Conicyt RI 2.0 (Comisión Nacional de Investigación Científica y Tecnológica)
op_collection_id ftconicyt
language Spanish
description Immunoglobulins (Igs) from sera of Atlantic salmon (Salmo salar) were purified with 50% saturated ammonium sulphate followed by molecular sieving chromatography using Sephacryl S-300. The fraction of purified Igs was used as immunogen to generate a polyclonal antibody anti-salmon Tgs. This antibody was affinity purified in a Sepharose-protein G column. By immunoradiometric assay against the immunogen, the rabbit IgG anti-salmon Igs showed a high specific titer (at least 1/100.000) for the antigen. This reagent offers the possibility of detection of salmon or trout (crossreactivity) humoral immune responses, both for diagnostic and research purposes.
format Article in Journal/Newspaper
author Ferreira-Vigouroux, Luis
Aguillón-Gutiérrez, Juan
Molina-Sampayo, María
Colombo, Alicia
Smith, Pedro
spellingShingle Ferreira-Vigouroux, Luis
Aguillón-Gutiérrez, Juan
Molina-Sampayo, María
Colombo, Alicia
Smith, Pedro
Polyclonal antibodies anti-salmon immunoglobulins; potential tools for diagnosis
author_facet Ferreira-Vigouroux, Luis
Aguillón-Gutiérrez, Juan
Molina-Sampayo, María
Colombo, Alicia
Smith, Pedro
author_sort Ferreira-Vigouroux, Luis
title Polyclonal antibodies anti-salmon immunoglobulins; potential tools for diagnosis
title_short Polyclonal antibodies anti-salmon immunoglobulins; potential tools for diagnosis
title_full Polyclonal antibodies anti-salmon immunoglobulins; potential tools for diagnosis
title_fullStr Polyclonal antibodies anti-salmon immunoglobulins; potential tools for diagnosis
title_full_unstemmed Polyclonal antibodies anti-salmon immunoglobulins; potential tools for diagnosis
title_sort polyclonal antibodies anti-salmon immunoglobulins; potential tools for diagnosis
publisher UNIV AUSTRAL CHILE, FAC CIENCIAS VETERINARIAS
url http://hdl.handle.net/10533/197442
op_coverage CHL
VALDIVIA
genre Atlantic salmon
Salmo salar
genre_facet Atlantic salmon
Salmo salar
op_source ARCHIVOS DE MEDICINA VETERINARIA
op_relation instname: Conicyt
reponame: Repositorio Digital RI2.0
info:eu-repo/grantAgreement/Fondef/D96T1023
info:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
http://hdl.handle.net/10533/197442
doi:0
wos: WOS:A1997XJ66200016
eissn: 0
issn: 0301-732X
op_rights info:eu-repo/semantics/openAccess
_version_ 1766362132400570368

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