Polyclonal antibodies anti-salmon immunoglobulins; potential tools for diagnosis

Immunoglobulins (Igs) from sera of Atlantic salmon (Salmo salar) were purified with 50% saturated ammonium sulphate followed by molecular sieving chromatography using Sephacryl S-300. The fraction of purified Igs was used as immunogen to generate a polyclonal antibody anti-salmon Tgs. This antibody...

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Bibliographic Details
Main Authors: Ferreira-Vigouroux, Luis, Aguillón-Gutiérrez, Juan, Molina-Sampayo, María, Colombo, Alicia, Smith, Pedro
Format: Article in Journal/Newspaper
Language:Spanish
Published: UNIV AUSTRAL CHILE, FAC CIENCIAS VETERINARIAS
Subjects:
Atlantic salmon
Salmo salar
Online Access:http://hdl.handle.net/10533/197442
Description
Summary:Immunoglobulins (Igs) from sera of Atlantic salmon (Salmo salar) were purified with 50% saturated ammonium sulphate followed by molecular sieving chromatography using Sephacryl S-300. The fraction of purified Igs was used as immunogen to generate a polyclonal antibody anti-salmon Tgs. This antibody was affinity purified in a Sepharose-protein G column. By immunoradiometric assay against the immunogen, the rabbit IgG anti-salmon Igs showed a high specific titer (at least 1/100.000) for the antigen. This reagent offers the possibility of detection of salmon or trout (crossreactivity) humoral immune responses, both for diagnostic and research purposes.

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