Bacterial community structure in a latitudinal gradient of lakes: The roles of spatial versus environmental factors
1.We analysed the latitudinal variation of bacterioplankton in 45 freshwater environments (lakes, shallow lakes and ponds) across a transect of more than 2100km stretching from Argentinean Patagonia (45°S) to Maritime Antarctica (63°S), to determine the factors that mainly determine bacterioplankton...
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ftconicet:oai:ri.conicet.gov.ar:11336/95897 2023-10-09T21:47:15+02:00 Bacterial community structure in a latitudinal gradient of lakes: The roles of spatial versus environmental factors Schiaffino, María Romina Unrein, Fernando Gasol, Josep M. Massana, Ramon Balagué, Vanessa Izaguirre, Irina application/pdf http://hdl.handle.net/11336/95897 eng eng Wiley Blackwell Publishing, Inc info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2427.2011.02628.x info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2427.2011.02628.x http://hdl.handle.net/11336/95897 Schiaffino, María Romina; Unrein, Fernando; Gasol, Josep M.; Massana, Ramon; Balagué, Vanessa; et al.; Bacterial community structure in a latitudinal gradient of lakes: The roles of spatial versus environmental factors; Wiley Blackwell Publishing, Inc; Freshwater Biology (print); 56; 10; 10-2011; 1973-1991 0046-5070 CONICET Digital CONICET info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ ANTARCTICA BACTERIOPLANKTON BIOGEOGRAPHY LAKES PATAGONIA https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1111/j.1365-2427.2011.02628.x 2023-09-24T19:14:16Z 1.We analysed the latitudinal variation of bacterioplankton in 45 freshwater environments (lakes, shallow lakes and ponds) across a transect of more than 2100km stretching from Argentinean Patagonia (45°S) to Maritime Antarctica (63°S), to determine the factors that mainly determine bacterioplankton community structure. 2.Bacterioplankton community composition (BCC) was assessed by a fingerprinting method (denaturing gradient gel electrophoresis) followed by band sequencing, whereas the abundances of total bacteria and picocyanobacteria were estimated by epifluorescence microscopy. 3.Bacterioplankton community composition was controlled by a combination of spatial (latitude and longitude) and environmental [e.g. phosphate, light diffuse attenuation coefficient (Kd) and dissolved organic carbon] factors. Total bacterioplankton abundance declined with latitude. A multiple regression analysis showed that phosphate, Kd and latitude had significant effects on total bacterioplankton abundance. 4.Of 76 operational taxonomic units identified in the studied lakes, 45 were shared between Patagonian and Antarctic water bodies, 28 were present only in Patagonian lakes and three were restricted to the Antarctic lakes. Significant differences were found in BCC between Patagonia and Antarctica. Among the sequences, 54% were similar (>97% sequence similarity) to others reported from cold habitats elsewhere on the planet (glaciers, high mountain lakes, Arctic). 5.Our results provide new evidence that supports the hypotheses of biogeographic patterns of bacterial assemblages and suggest that both spatial and environmental factors control bacterioplankton community structure. Fil: Schiaffino, María Romina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Limnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Arctic CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Arctic Antarctic The Antarctic Patagonia Argentina Freshwater Biology 56 10 1973 1991 |
institution |
Open Polar |
collection |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
op_collection_id |
ftconicet |
language |
English |
topic |
ANTARCTICA BACTERIOPLANKTON BIOGEOGRAPHY LAKES PATAGONIA https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
spellingShingle |
ANTARCTICA BACTERIOPLANKTON BIOGEOGRAPHY LAKES PATAGONIA https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Schiaffino, María Romina Unrein, Fernando Gasol, Josep M. Massana, Ramon Balagué, Vanessa Izaguirre, Irina Bacterial community structure in a latitudinal gradient of lakes: The roles of spatial versus environmental factors |
topic_facet |
ANTARCTICA BACTERIOPLANKTON BIOGEOGRAPHY LAKES PATAGONIA https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
description |
1.We analysed the latitudinal variation of bacterioplankton in 45 freshwater environments (lakes, shallow lakes and ponds) across a transect of more than 2100km stretching from Argentinean Patagonia (45°S) to Maritime Antarctica (63°S), to determine the factors that mainly determine bacterioplankton community structure. 2.Bacterioplankton community composition (BCC) was assessed by a fingerprinting method (denaturing gradient gel electrophoresis) followed by band sequencing, whereas the abundances of total bacteria and picocyanobacteria were estimated by epifluorescence microscopy. 3.Bacterioplankton community composition was controlled by a combination of spatial (latitude and longitude) and environmental [e.g. phosphate, light diffuse attenuation coefficient (Kd) and dissolved organic carbon] factors. Total bacterioplankton abundance declined with latitude. A multiple regression analysis showed that phosphate, Kd and latitude had significant effects on total bacterioplankton abundance. 4.Of 76 operational taxonomic units identified in the studied lakes, 45 were shared between Patagonian and Antarctic water bodies, 28 were present only in Patagonian lakes and three were restricted to the Antarctic lakes. Significant differences were found in BCC between Patagonia and Antarctica. Among the sequences, 54% were similar (>97% sequence similarity) to others reported from cold habitats elsewhere on the planet (glaciers, high mountain lakes, Arctic). 5.Our results provide new evidence that supports the hypotheses of biogeographic patterns of bacterial assemblages and suggest that both spatial and environmental factors control bacterioplankton community structure. Fil: Schiaffino, María Romina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Limnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de ... |
format |
Article in Journal/Newspaper |
author |
Schiaffino, María Romina Unrein, Fernando Gasol, Josep M. Massana, Ramon Balagué, Vanessa Izaguirre, Irina |
author_facet |
Schiaffino, María Romina Unrein, Fernando Gasol, Josep M. Massana, Ramon Balagué, Vanessa Izaguirre, Irina |
author_sort |
Schiaffino, María Romina |
title |
Bacterial community structure in a latitudinal gradient of lakes: The roles of spatial versus environmental factors |
title_short |
Bacterial community structure in a latitudinal gradient of lakes: The roles of spatial versus environmental factors |
title_full |
Bacterial community structure in a latitudinal gradient of lakes: The roles of spatial versus environmental factors |
title_fullStr |
Bacterial community structure in a latitudinal gradient of lakes: The roles of spatial versus environmental factors |
title_full_unstemmed |
Bacterial community structure in a latitudinal gradient of lakes: The roles of spatial versus environmental factors |
title_sort |
bacterial community structure in a latitudinal gradient of lakes: the roles of spatial versus environmental factors |
publisher |
Wiley Blackwell Publishing, Inc |
url |
http://hdl.handle.net/11336/95897 |
geographic |
Arctic Antarctic The Antarctic Patagonia Argentina |
geographic_facet |
Arctic Antarctic The Antarctic Patagonia Argentina |
genre |
Antarc* Antarctic Antarctica Arctic |
genre_facet |
Antarc* Antarctic Antarctica Arctic |
op_relation |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2427.2011.02628.x info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2427.2011.02628.x http://hdl.handle.net/11336/95897 Schiaffino, María Romina; Unrein, Fernando; Gasol, Josep M.; Massana, Ramon; Balagué, Vanessa; et al.; Bacterial community structure in a latitudinal gradient of lakes: The roles of spatial versus environmental factors; Wiley Blackwell Publishing, Inc; Freshwater Biology (print); 56; 10; 10-2011; 1973-1991 0046-5070 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1111/j.1365-2427.2011.02628.x |
container_title |
Freshwater Biology |
container_volume |
56 |
container_issue |
10 |
container_start_page |
1973 |
op_container_end_page |
1991 |
_version_ |
1779310231850844160 |