Effect of operating variables and kinetics of the lipase catalyzed transesterification of ethylene carbonate and glycerol
Glycerol carbonate (GC) is a value-added product originating from the valorization of widely available glycerol (Gly), a side stream from the production of biodiesel. Here we approach the production of this chemical comparing two reactions based on the transesterification of Gly with dimethyl carbon...
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ftconicet:oai:ri.conicet.gov.ar:11336/90798 2023-10-09T21:46:01+02:00 Effect of operating variables and kinetics of the lipase catalyzed transesterification of ethylene carbonate and glycerol Gutierrez Lazaro, Ana Velasco, Daniel Boldrini, Diego Emmanuel Yustos, Pedro Esteban, Jesús Ladero, Miguel application/pdf http://hdl.handle.net/11336/90798 eng eng MDPI AG info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2311-5637/4/3/75 info:eu-repo/semantics/altIdentifier/doi/10.3390/fermentation4030075 http://hdl.handle.net/11336/90798 Gutierrez Lazaro, Ana; Velasco, Daniel; Boldrini, Diego Emmanuel; Yustos, Pedro; Esteban, Jesús; et al.; Effect of operating variables and kinetics of the lipase catalyzed transesterification of ethylene carbonate and glycerol; MDPI AG; Fermentation; 4; 3; 5-9-2018; 1-14 2311-5637 CONICET Digital CONICET info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ EVERSA TRANSFORM 2.0 GLYCEROL GLYCEROL CARBONATE KINETIC MODEL LIPOZYME TL 100 L NOVOZYM 435 https://purl.org/becyt/ford/2.4 https://purl.org/becyt/ford/2 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.3390/fermentation4030075 2023-09-24T20:21:13Z Glycerol carbonate (GC) is a value-added product originating from the valorization of widely available glycerol (Gly), a side stream from the production of biodiesel. Here we approach the production of this chemical comparing two reactions based on the transesterification of Gly with dimethyl carbonate (DMC) and ethylene carbonate (EC). For the former reaction, it was observed that the free enzyme CALB (lipase B from Candida antarctica) gave the best results, whereas for the latter, Eversa Transform (a genetic modification of Thermomyces lanuginosus lipase) performed better than the rest. With the selected catalysts, their immobilized analogous enzymes Novozym 435 and Lypozyme TL IM, respectively, were also tested. Observing that the yields for the reaction with EC were significantly faster, other operating variables were evaluated, resulting the best performance using a closed system, tert-butanol as solvent, a concentration of enzyme Eversa Transform of 3 % w/w, a molar excess of EC:Gly of 9:1 and a temperature of 60 C. Finally, several runs were conducted at different temperatures and molar ratios of EC:Gly, fitting a kinetic model to all experimental data for the reaction catalyzed with Eversa Transform. This model included the bimolecular transesterification reaction of Gly and EC catalyzed by the lipase and a reversible ring-opening polymerization of EC. Fil: Gutierrez Lazaro, Ana. Complutense University of Madrid; España Fil: Velasco, Daniel. Complutense University of Madrid; España Fil: Boldrini, Diego Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina Fil: Yustos, Pedro. Complutense University of Madrid; España Fil: Esteban, Jesús. Max Planck Institute for Chemical Energy Conversion. Molecular Catalysis Division; Alemania Fil: Ladero, Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro ... Article in Journal/Newspaper Antarc* Antarctica CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Argentina Fermentation 4 3 75 |
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CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
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language |
English |
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EVERSA TRANSFORM 2.0 GLYCEROL GLYCEROL CARBONATE KINETIC MODEL LIPOZYME TL 100 L NOVOZYM 435 https://purl.org/becyt/ford/2.4 https://purl.org/becyt/ford/2 |
spellingShingle |
EVERSA TRANSFORM 2.0 GLYCEROL GLYCEROL CARBONATE KINETIC MODEL LIPOZYME TL 100 L NOVOZYM 435 https://purl.org/becyt/ford/2.4 https://purl.org/becyt/ford/2 Gutierrez Lazaro, Ana Velasco, Daniel Boldrini, Diego Emmanuel Yustos, Pedro Esteban, Jesús Ladero, Miguel Effect of operating variables and kinetics of the lipase catalyzed transesterification of ethylene carbonate and glycerol |
topic_facet |
EVERSA TRANSFORM 2.0 GLYCEROL GLYCEROL CARBONATE KINETIC MODEL LIPOZYME TL 100 L NOVOZYM 435 https://purl.org/becyt/ford/2.4 https://purl.org/becyt/ford/2 |
description |
Glycerol carbonate (GC) is a value-added product originating from the valorization of widely available glycerol (Gly), a side stream from the production of biodiesel. Here we approach the production of this chemical comparing two reactions based on the transesterification of Gly with dimethyl carbonate (DMC) and ethylene carbonate (EC). For the former reaction, it was observed that the free enzyme CALB (lipase B from Candida antarctica) gave the best results, whereas for the latter, Eversa Transform (a genetic modification of Thermomyces lanuginosus lipase) performed better than the rest. With the selected catalysts, their immobilized analogous enzymes Novozym 435 and Lypozyme TL IM, respectively, were also tested. Observing that the yields for the reaction with EC were significantly faster, other operating variables were evaluated, resulting the best performance using a closed system, tert-butanol as solvent, a concentration of enzyme Eversa Transform of 3 % w/w, a molar excess of EC:Gly of 9:1 and a temperature of 60 C. Finally, several runs were conducted at different temperatures and molar ratios of EC:Gly, fitting a kinetic model to all experimental data for the reaction catalyzed with Eversa Transform. This model included the bimolecular transesterification reaction of Gly and EC catalyzed by the lipase and a reversible ring-opening polymerization of EC. Fil: Gutierrez Lazaro, Ana. Complutense University of Madrid; España Fil: Velasco, Daniel. Complutense University of Madrid; España Fil: Boldrini, Diego Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina Fil: Yustos, Pedro. Complutense University of Madrid; España Fil: Esteban, Jesús. Max Planck Institute for Chemical Energy Conversion. Molecular Catalysis Division; Alemania Fil: Ladero, Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro ... |
format |
Article in Journal/Newspaper |
author |
Gutierrez Lazaro, Ana Velasco, Daniel Boldrini, Diego Emmanuel Yustos, Pedro Esteban, Jesús Ladero, Miguel |
author_facet |
Gutierrez Lazaro, Ana Velasco, Daniel Boldrini, Diego Emmanuel Yustos, Pedro Esteban, Jesús Ladero, Miguel |
author_sort |
Gutierrez Lazaro, Ana |
title |
Effect of operating variables and kinetics of the lipase catalyzed transesterification of ethylene carbonate and glycerol |
title_short |
Effect of operating variables and kinetics of the lipase catalyzed transesterification of ethylene carbonate and glycerol |
title_full |
Effect of operating variables and kinetics of the lipase catalyzed transesterification of ethylene carbonate and glycerol |
title_fullStr |
Effect of operating variables and kinetics of the lipase catalyzed transesterification of ethylene carbonate and glycerol |
title_full_unstemmed |
Effect of operating variables and kinetics of the lipase catalyzed transesterification of ethylene carbonate and glycerol |
title_sort |
effect of operating variables and kinetics of the lipase catalyzed transesterification of ethylene carbonate and glycerol |
publisher |
MDPI AG |
url |
http://hdl.handle.net/11336/90798 |
geographic |
Argentina |
geographic_facet |
Argentina |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2311-5637/4/3/75 info:eu-repo/semantics/altIdentifier/doi/10.3390/fermentation4030075 http://hdl.handle.net/11336/90798 Gutierrez Lazaro, Ana; Velasco, Daniel; Boldrini, Diego Emmanuel; Yustos, Pedro; Esteban, Jesús; et al.; Effect of operating variables and kinetics of the lipase catalyzed transesterification of ethylene carbonate and glycerol; MDPI AG; Fermentation; 4; 3; 5-9-2018; 1-14 2311-5637 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
op_doi |
https://doi.org/10.3390/fermentation4030075 |
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Fermentation |
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4 |
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3 |
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75 |
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1779321677388185600 |