Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration
Axonal degeneration occurs in the developing nervous system for the appropriate establishment of mature circuits, and is also a hallmark of diverse neurodegenerative diseases. Despite recent interest in the field, little is known about the changes (and possible role) of the cytoskeleton during axona...
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ftconicet:oai:ri.conicet.gov.ar:11336/90541 2023-10-09T21:55:47+02:00 Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration Unsain, Nicolas Bordenave, Martín Diego Martinez, Gaby F. Jalil, Sami Von Bilderling, Catalina Barabas, Federico Martín Masullo, Luciano Andrés Johnstone, Aaron D. Barker, Philip A. Bisbal, Mariano Stefani, Fernando Daniel Caceres, Alfredo Oscar application/pdf http://hdl.handle.net/11336/90541 eng eng Nature Publishing Group info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-21232-0 info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-018-21232-0 http://hdl.handle.net/11336/90541 Unsain, Nicolas; Bordenave, Martín Diego; Martinez, Gaby F.; Jalil, Sami; Von Bilderling, Catalina; et al.; Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-16 2045-2322 CONICET Digital CONICET info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ NANOSCOPY AXON DEGENERATION NEURONS https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1038/s41598-018-21232-0 2023-09-24T19:05:47Z Axonal degeneration occurs in the developing nervous system for the appropriate establishment of mature circuits, and is also a hallmark of diverse neurodegenerative diseases. Despite recent interest in the field, little is known about the changes (and possible role) of the cytoskeleton during axonal degeneration. We studied the actin cytoskeleton in an in vitro model of developmental pruning induced by trophic factor withdrawal (TFW). We found that F-actin decrease and growth cone collapse (GCC) occur early after TFW; however, treatments that prevent axonal fragmentation failed to prevent GCC, suggesting independent pathways. Using super-resolution (STED) microscopy we found that the axonal actin/spectrin membrane-associated periodic skeleton (MPS) abundance and organization drop shortly after deprivation, remaining low until fragmentation. Fragmented axons lack MPS (while maintaining microtubules) and acute pharmacological treatments that stabilize actin filaments prevent MPS loss and protect from axonal fragmentation, suggesting that MPS destruction is required for axon fragmentation to proceed. Fil: Unsain, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Bordenave, Martín Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina Fil: Martinez, Gaby F. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Jalil, Sami. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico ... Article in Journal/Newspaper sami CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Argentina Gaby ENVELOPE(-81.183,-81.183,51.200,51.200) Scientific Reports 8 1 |
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CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
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NANOSCOPY AXON DEGENERATION NEURONS https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
spellingShingle |
NANOSCOPY AXON DEGENERATION NEURONS https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Unsain, Nicolas Bordenave, Martín Diego Martinez, Gaby F. Jalil, Sami Von Bilderling, Catalina Barabas, Federico Martín Masullo, Luciano Andrés Johnstone, Aaron D. Barker, Philip A. Bisbal, Mariano Stefani, Fernando Daniel Caceres, Alfredo Oscar Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration |
topic_facet |
NANOSCOPY AXON DEGENERATION NEURONS https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
description |
Axonal degeneration occurs in the developing nervous system for the appropriate establishment of mature circuits, and is also a hallmark of diverse neurodegenerative diseases. Despite recent interest in the field, little is known about the changes (and possible role) of the cytoskeleton during axonal degeneration. We studied the actin cytoskeleton in an in vitro model of developmental pruning induced by trophic factor withdrawal (TFW). We found that F-actin decrease and growth cone collapse (GCC) occur early after TFW; however, treatments that prevent axonal fragmentation failed to prevent GCC, suggesting independent pathways. Using super-resolution (STED) microscopy we found that the axonal actin/spectrin membrane-associated periodic skeleton (MPS) abundance and organization drop shortly after deprivation, remaining low until fragmentation. Fragmented axons lack MPS (while maintaining microtubules) and acute pharmacological treatments that stabilize actin filaments prevent MPS loss and protect from axonal fragmentation, suggesting that MPS destruction is required for axon fragmentation to proceed. Fil: Unsain, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Bordenave, Martín Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina Fil: Martinez, Gaby F. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Jalil, Sami. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico ... |
format |
Article in Journal/Newspaper |
author |
Unsain, Nicolas Bordenave, Martín Diego Martinez, Gaby F. Jalil, Sami Von Bilderling, Catalina Barabas, Federico Martín Masullo, Luciano Andrés Johnstone, Aaron D. Barker, Philip A. Bisbal, Mariano Stefani, Fernando Daniel Caceres, Alfredo Oscar |
author_facet |
Unsain, Nicolas Bordenave, Martín Diego Martinez, Gaby F. Jalil, Sami Von Bilderling, Catalina Barabas, Federico Martín Masullo, Luciano Andrés Johnstone, Aaron D. Barker, Philip A. Bisbal, Mariano Stefani, Fernando Daniel Caceres, Alfredo Oscar |
author_sort |
Unsain, Nicolas |
title |
Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration |
title_short |
Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration |
title_full |
Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration |
title_fullStr |
Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration |
title_full_unstemmed |
Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration |
title_sort |
remodeling of the actin/spectrin membrane-associated periodic skeleton, growth cone collapse and f-actin decrease during axonal degeneration |
publisher |
Nature Publishing Group |
url |
http://hdl.handle.net/11336/90541 |
long_lat |
ENVELOPE(-81.183,-81.183,51.200,51.200) |
geographic |
Argentina Gaby |
geographic_facet |
Argentina Gaby |
genre |
sami |
genre_facet |
sami |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-21232-0 info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-018-21232-0 http://hdl.handle.net/11336/90541 Unsain, Nicolas; Bordenave, Martín Diego; Martinez, Gaby F.; Jalil, Sami; Von Bilderling, Catalina; et al.; Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-16 2045-2322 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1038/s41598-018-21232-0 |
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Scientific Reports |
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8 |
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1 |
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1779319859304202240 |