Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements
A hyphenated system was developed for Hg(ii) and methylmercury (MeHg) preconcentration and speciation analysis by the online coupling of solid phase extraction (SPE), high performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS). Both analytes, Hg(ii) and Me...
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ftconicet:oai:ri.conicet.gov.ar:11336/87358 2023-10-09T21:44:52+02:00 Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements Londonio, Juan Agustín Hasuoka, Paul Emir Pacheco, Pablo Hugo Gil, Raul Andres Smichowski, Patricia Nora application/pdf http://hdl.handle.net/11336/87358 eng eng Royal Society of Chemistry info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2018/ja/c8ja00188j#!divAbstract info:eu-repo/semantics/altIdentifier/doi/10.1039/C8JA00188J http://hdl.handle.net/11336/87358 Londonio, Juan Agustín; Hasuoka, Paul Emir; Pacheco, Pablo Hugo; Gil, Raul Andres; Smichowski, Patricia Nora; Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements; Royal Society of Chemistry; Journal of Analytical Atomic Spectrometry; 33; 10; 10-2018; 1737-1744 0267-9477 CONICET Digital CONICET info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ Mercury speciation carbon nanotubes flow injection analysis LC-ICPMS https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1039/C8JA00188J 2023-09-24T19:47:37Z A hyphenated system was developed for Hg(ii) and methylmercury (MeHg) preconcentration and speciation analysis by the online coupling of solid phase extraction (SPE), high performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS). Both analytes, Hg(ii) and MeHg, were preconcentrated on a microcolumn filled with multiwalled carbon nanotubes (MWCNTs) functionalised with poly-l-methionine (polymet-MWCNTs). During the method development the sorbent material was carefully studied and the solid phase extraction conditions were optimised. An enrichment factor of 190 for Hg(ii) and MeHg was obtained when 20 mL of sample was passed through the microcolumn. For the chromatographic separation, a mobile phase composed of a ternary mixture of 0.5% formic acid + 0.2% 2-mercaptoethanol + 20% methanol was used. Separation of both mercurial species was accomplished in ∼10 min on a 250 mm C18 column. The detection limits of the SPE-HPLC-ICP-MS method were 15 ng L−1 for Hg(ii) and 17 ng L−1 for MeHg. The relative standard deviations of peak area for 5 ng L−1 of each Hg species were below 5%. Recoveries of Hg(ii) and MeHg were never less than 93%. For checking the accuracy, BCR 643-tuna fish and TORT-3 lobster hepatopancreas certificate reference materials were analysed. Mercury species were determined in spiked fish oil-based dietary supplements and Antarctic water. Fil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación en Ingeniería Ambiental; Argentina Fil: Hasuoka, Paul Emir. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Pacheco, Pablo Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San ... Article in Journal/Newspaper Antarc* Antarctic CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Antarctic Argentina San Martín ENVELOPE(-67.133,-67.133,-68.117,-68.117) Pablo ENVELOPE(-63.717,-63.717,-64.283,-64.283) Journal of Analytical Atomic Spectrometry 33 10 1737 1744 |
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Open Polar |
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CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
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ftconicet |
language |
English |
topic |
Mercury speciation carbon nanotubes flow injection analysis LC-ICPMS https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
spellingShingle |
Mercury speciation carbon nanotubes flow injection analysis LC-ICPMS https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 Londonio, Juan Agustín Hasuoka, Paul Emir Pacheco, Pablo Hugo Gil, Raul Andres Smichowski, Patricia Nora Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements |
topic_facet |
Mercury speciation carbon nanotubes flow injection analysis LC-ICPMS https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
description |
A hyphenated system was developed for Hg(ii) and methylmercury (MeHg) preconcentration and speciation analysis by the online coupling of solid phase extraction (SPE), high performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS). Both analytes, Hg(ii) and MeHg, were preconcentrated on a microcolumn filled with multiwalled carbon nanotubes (MWCNTs) functionalised with poly-l-methionine (polymet-MWCNTs). During the method development the sorbent material was carefully studied and the solid phase extraction conditions were optimised. An enrichment factor of 190 for Hg(ii) and MeHg was obtained when 20 mL of sample was passed through the microcolumn. For the chromatographic separation, a mobile phase composed of a ternary mixture of 0.5% formic acid + 0.2% 2-mercaptoethanol + 20% methanol was used. Separation of both mercurial species was accomplished in ∼10 min on a 250 mm C18 column. The detection limits of the SPE-HPLC-ICP-MS method were 15 ng L−1 for Hg(ii) and 17 ng L−1 for MeHg. The relative standard deviations of peak area for 5 ng L−1 of each Hg species were below 5%. Recoveries of Hg(ii) and MeHg were never less than 93%. For checking the accuracy, BCR 643-tuna fish and TORT-3 lobster hepatopancreas certificate reference materials were analysed. Mercury species were determined in spiked fish oil-based dietary supplements and Antarctic water. Fil: Londonio, Juan Agustín. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Instituto de Investigación en Ingeniería Ambiental; Argentina Fil: Hasuoka, Paul Emir. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Pacheco, Pablo Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San ... |
format |
Article in Journal/Newspaper |
author |
Londonio, Juan Agustín Hasuoka, Paul Emir Pacheco, Pablo Hugo Gil, Raul Andres Smichowski, Patricia Nora |
author_facet |
Londonio, Juan Agustín Hasuoka, Paul Emir Pacheco, Pablo Hugo Gil, Raul Andres Smichowski, Patricia Nora |
author_sort |
Londonio, Juan Agustín |
title |
Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements |
title_short |
Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements |
title_full |
Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements |
title_fullStr |
Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements |
title_full_unstemmed |
Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements |
title_sort |
online solid phase extraction-hplc-icp-ms system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements |
publisher |
Royal Society of Chemistry |
url |
http://hdl.handle.net/11336/87358 |
long_lat |
ENVELOPE(-67.133,-67.133,-68.117,-68.117) ENVELOPE(-63.717,-63.717,-64.283,-64.283) |
geographic |
Antarctic Argentina San Martín Pablo |
geographic_facet |
Antarctic Argentina San Martín Pablo |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2018/ja/c8ja00188j#!divAbstract info:eu-repo/semantics/altIdentifier/doi/10.1039/C8JA00188J http://hdl.handle.net/11336/87358 Londonio, Juan Agustín; Hasuoka, Paul Emir; Pacheco, Pablo Hugo; Gil, Raul Andres; Smichowski, Patricia Nora; Online solid phase extraction-HPLC-ICP-MS system for mercury and methylmercury preconcentration using functionalised carbon nanotubes for their determination in dietary supplements; Royal Society of Chemistry; Journal of Analytical Atomic Spectrometry; 33; 10; 10-2018; 1737-1744 0267-9477 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
op_doi |
https://doi.org/10.1039/C8JA00188J |
container_title |
Journal of Analytical Atomic Spectrometry |
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33 |
container_issue |
10 |
container_start_page |
1737 |
op_container_end_page |
1744 |
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1779314146693611520 |