Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella
This study focuses on the utility of molecular markers for the discrimination of closely related species in tintinnid ciliates. We analyzed the ecologically important genus Helicostomella by sequencing part of the large-subunit rDNA (LSU rDNA) and the 5.8S rDNA combined with the internally transcrib...
Published in: | Protist |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier Gmbh
|
Subjects: | |
Online Access: | http://hdl.handle.net/11336/85271 |
id |
ftconicet:oai:ri.conicet.gov.ar:11336/85271 |
---|---|
record_format |
openpolar |
spelling |
ftconicet:oai:ri.conicet.gov.ar:11336/85271 2023-05-15T16:01:01+02:00 Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella Santoferrara, Luciana Fernanda Tian, Michael Alder, Viviana Andrea McManus, George B. application/pdf http://hdl.handle.net/11336/85271 eng eng Elsevier Gmbh info:eu-repo/semantics/altIdentifier/doi/10.1016/j.protis.2014.11.005 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1434461014001217 Santoferrara, Luciana Fernanda; Tian, Michael; Alder, Viviana Andrea; McManus, George B.; Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella; Elsevier Gmbh; Protist; 166; 1; 2-2015; 78-92 1434-4610 http://hdl.handle.net/11336/85271 CONICET Digital CONICET info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ CC-BY-NC-ND CLOSELY RELATED SPECIES CRYPTIC SPECIES DNA BARCODING MOLECULAR MARKERS POLYMORPHIC SPECIES TINTINNID CILIATE Biología Celular Microbiología Ciencias Biológicas CIENCIAS NATURALES Y EXACTAS info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1016/j.protis.2014.11.005 2019-10-09T23:59:53Z This study focuses on the utility of molecular markers for the discrimination of closely related species in tintinnid ciliates. We analyzed the ecologically important genus Helicostomella by sequencing part of the large-subunit rDNA (LSU rDNA) and the 5.8S rDNA combined with the internally transcribed spacer regions 1 and 2 (5.8S rDNA-ITS) from forty-five individuals collected in NW and SW Atlantic waters and after culturing. Although all described Helicostomella species represent a continuum of morphologies, forms with shorter or longer loricae would correspond to different species according to previous molecular data. Here we observed that long forms show both crypticity (i.e. two almost identical long forms with different DNA sequences) and polymorphism (i.e. some long forms develop significantly shorter loricae after culturing). Reviewing all available tintinnid sequences, we found that 1) three Helicostomella clusters are consistent with different species from a molecular perspective, although these clusters are neither clearly differentiated by their loricae nor unambiguously linked to described species, 2) Helicostomella is closely related (probably to the family or genus level) to four ". Tintinnopsis-like" morphospecies, and 3) if considered separately, neither LSU rDNA nor 5.8S rDNA-ITS completely discriminate closely related species, thus supporting the use of multi-gene barcodes for tintinnids. Fil: Santoferrara, Luciana Fernanda. University of Connecticut; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tian, Michael. University of Connecticut; Estados Unidos Fil: Alder, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina Fil: McManus, George B. University of Connecticut; Estados Unidos Article in Journal/Newspaper Dirección Nacional del Antártico Instituto Antártico Argentino CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Argentina Argentino Protist 166 1 78 92 |
institution |
Open Polar |
collection |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
op_collection_id |
ftconicet |
language |
English |
topic |
CLOSELY RELATED SPECIES CRYPTIC SPECIES DNA BARCODING MOLECULAR MARKERS POLYMORPHIC SPECIES TINTINNID CILIATE Biología Celular Microbiología Ciencias Biológicas CIENCIAS NATURALES Y EXACTAS |
spellingShingle |
CLOSELY RELATED SPECIES CRYPTIC SPECIES DNA BARCODING MOLECULAR MARKERS POLYMORPHIC SPECIES TINTINNID CILIATE Biología Celular Microbiología Ciencias Biológicas CIENCIAS NATURALES Y EXACTAS Santoferrara, Luciana Fernanda Tian, Michael Alder, Viviana Andrea McManus, George B. Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella |
topic_facet |
CLOSELY RELATED SPECIES CRYPTIC SPECIES DNA BARCODING MOLECULAR MARKERS POLYMORPHIC SPECIES TINTINNID CILIATE Biología Celular Microbiología Ciencias Biológicas CIENCIAS NATURALES Y EXACTAS |
description |
This study focuses on the utility of molecular markers for the discrimination of closely related species in tintinnid ciliates. We analyzed the ecologically important genus Helicostomella by sequencing part of the large-subunit rDNA (LSU rDNA) and the 5.8S rDNA combined with the internally transcribed spacer regions 1 and 2 (5.8S rDNA-ITS) from forty-five individuals collected in NW and SW Atlantic waters and after culturing. Although all described Helicostomella species represent a continuum of morphologies, forms with shorter or longer loricae would correspond to different species according to previous molecular data. Here we observed that long forms show both crypticity (i.e. two almost identical long forms with different DNA sequences) and polymorphism (i.e. some long forms develop significantly shorter loricae after culturing). Reviewing all available tintinnid sequences, we found that 1) three Helicostomella clusters are consistent with different species from a molecular perspective, although these clusters are neither clearly differentiated by their loricae nor unambiguously linked to described species, 2) Helicostomella is closely related (probably to the family or genus level) to four ". Tintinnopsis-like" morphospecies, and 3) if considered separately, neither LSU rDNA nor 5.8S rDNA-ITS completely discriminate closely related species, thus supporting the use of multi-gene barcodes for tintinnids. Fil: Santoferrara, Luciana Fernanda. University of Connecticut; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tian, Michael. University of Connecticut; Estados Unidos Fil: Alder, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina Fil: McManus, George B. University of Connecticut; Estados Unidos |
format |
Article in Journal/Newspaper |
author |
Santoferrara, Luciana Fernanda Tian, Michael Alder, Viviana Andrea McManus, George B. |
author_facet |
Santoferrara, Luciana Fernanda Tian, Michael Alder, Viviana Andrea McManus, George B. |
author_sort |
Santoferrara, Luciana Fernanda |
title |
Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella |
title_short |
Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella |
title_full |
Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella |
title_fullStr |
Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella |
title_full_unstemmed |
Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella |
title_sort |
discrimination of closely related species in tintinnid ciliates: new insights on crypticity and polymorphism in the genus helicostomella |
publisher |
Elsevier Gmbh |
url |
http://hdl.handle.net/11336/85271 |
geographic |
Argentina Argentino |
geographic_facet |
Argentina Argentino |
genre |
Dirección Nacional del Antártico Instituto Antártico Argentino |
genre_facet |
Dirección Nacional del Antártico Instituto Antártico Argentino |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.protis.2014.11.005 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1434461014001217 Santoferrara, Luciana Fernanda; Tian, Michael; Alder, Viviana Andrea; McManus, George B.; Discrimination of closely related species in tintinnid ciliates: New insights on crypticity and polymorphism in the genus helicostomella; Elsevier Gmbh; Protist; 166; 1; 2-2015; 78-92 1434-4610 http://hdl.handle.net/11336/85271 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.protis.2014.11.005 |
container_title |
Protist |
container_volume |
166 |
container_issue |
1 |
container_start_page |
78 |
op_container_end_page |
92 |
_version_ |
1766397014964174848 |