Influence of the nature of the support on the catalytic performance of CALB: Experimental and theoretical evidence
The immobilization of the lipase B of Candida antarctica (CALB) was carried out on various hydrophilic and hydrophobic supports through adsorption and covalent bond interactions. The catalytic performance of the biocatalysts was evaluated in the enantioselective esterification of R/S-ibuprofen with...
Published in: | Catalysis Science & Technology |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Royal Society of Chemistry
|
Subjects: | |
Online Access: | http://hdl.handle.net/11336/83396 |
id |
ftconicet:oai:ri.conicet.gov.ar:11336/83396 |
---|---|
record_format |
openpolar |
spelling |
ftconicet:oai:ri.conicet.gov.ar:11336/83396 2023-10-09T21:45:17+02:00 Influence of the nature of the support on the catalytic performance of CALB: Experimental and theoretical evidence José, Carla Toledo, Maria Victoria Nicolás, Paula Lasalle, Verónica Ferreira, María Luján Briand, Laura Estefania application/pdf http://hdl.handle.net/11336/83396 eng eng Royal Society of Chemistry info:eu-repo/semantics/altIdentifier/doi/10.1039/C7CY02466E info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2018/cy/c7cy02466e http://hdl.handle.net/11336/83396 José, Carla; Toledo, Maria Victoria; Nicolás, Paula; Lasalle, Verónica; Ferreira, María Luján; et al.; Influence of the nature of the support on the catalytic performance of CALB: Experimental and theoretical evidence; Royal Society of Chemistry; Catalysis Science and Technology; 8; 14; 21-7-2018; 3513-3526 2044-4761 CONICET Digital CONICET info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ CALB immobilized enzymes hydrophobic support hydrophilic support R/S-ibuprofen esterification https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1039/C7CY02466E 2023-09-24T18:59:02Z The immobilization of the lipase B of Candida antarctica (CALB) was carried out on various hydrophilic and hydrophobic supports through adsorption and covalent bond interactions. The catalytic performance of the biocatalysts was evaluated in the enantioselective esterification of R/S-ibuprofen with ethanol without co-solvents added. The present investigation demonstrates that the biocatalysts based on CALB immobilized by adsorption on hydrophobic supports (acrylic resin, polymethylmethacrylate, polystyrene, epoxy resin and polypropylene) exhibit higher conversion of ibuprofen and enantiomeric excess towards the S-enantiomer than CALB immobilized on hydrophilic materials. Additionally, the enzyme loading is a key factor influencing the conversion of the substrate regardless of the nature of the support material. A high enzyme loading is detrimental to the specific activity of the biocatalysts. Experimental and molecular modeling investigations provided evidence for the influence of water activity in the stereospecificity of the lipase. Fil: José, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina Fil: Toledo, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina Fil: Nicolás, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina Fil: Lasalle, Verónica. Consejo Nacional ... Article in Journal/Newspaper Antarc* Antarctica CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Argentina Toledo ENVELOPE(-67.317,-67.317,-73.700,-73.700) Catalysis Science & Technology 8 14 3513 3526 |
institution |
Open Polar |
collection |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
op_collection_id |
ftconicet |
language |
English |
topic |
CALB immobilized enzymes hydrophobic support hydrophilic support R/S-ibuprofen esterification https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
spellingShingle |
CALB immobilized enzymes hydrophobic support hydrophilic support R/S-ibuprofen esterification https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 José, Carla Toledo, Maria Victoria Nicolás, Paula Lasalle, Verónica Ferreira, María Luján Briand, Laura Estefania Influence of the nature of the support on the catalytic performance of CALB: Experimental and theoretical evidence |
topic_facet |
CALB immobilized enzymes hydrophobic support hydrophilic support R/S-ibuprofen esterification https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
description |
The immobilization of the lipase B of Candida antarctica (CALB) was carried out on various hydrophilic and hydrophobic supports through adsorption and covalent bond interactions. The catalytic performance of the biocatalysts was evaluated in the enantioselective esterification of R/S-ibuprofen with ethanol without co-solvents added. The present investigation demonstrates that the biocatalysts based on CALB immobilized by adsorption on hydrophobic supports (acrylic resin, polymethylmethacrylate, polystyrene, epoxy resin and polypropylene) exhibit higher conversion of ibuprofen and enantiomeric excess towards the S-enantiomer than CALB immobilized on hydrophilic materials. Additionally, the enzyme loading is a key factor influencing the conversion of the substrate regardless of the nature of the support material. A high enzyme loading is detrimental to the specific activity of the biocatalysts. Experimental and molecular modeling investigations provided evidence for the influence of water activity in the stereospecificity of the lipase. Fil: José, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina Fil: Toledo, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina Fil: Nicolás, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina Fil: Lasalle, Verónica. Consejo Nacional ... |
format |
Article in Journal/Newspaper |
author |
José, Carla Toledo, Maria Victoria Nicolás, Paula Lasalle, Verónica Ferreira, María Luján Briand, Laura Estefania |
author_facet |
José, Carla Toledo, Maria Victoria Nicolás, Paula Lasalle, Verónica Ferreira, María Luján Briand, Laura Estefania |
author_sort |
José, Carla |
title |
Influence of the nature of the support on the catalytic performance of CALB: Experimental and theoretical evidence |
title_short |
Influence of the nature of the support on the catalytic performance of CALB: Experimental and theoretical evidence |
title_full |
Influence of the nature of the support on the catalytic performance of CALB: Experimental and theoretical evidence |
title_fullStr |
Influence of the nature of the support on the catalytic performance of CALB: Experimental and theoretical evidence |
title_full_unstemmed |
Influence of the nature of the support on the catalytic performance of CALB: Experimental and theoretical evidence |
title_sort |
influence of the nature of the support on the catalytic performance of calb: experimental and theoretical evidence |
publisher |
Royal Society of Chemistry |
url |
http://hdl.handle.net/11336/83396 |
long_lat |
ENVELOPE(-67.317,-67.317,-73.700,-73.700) |
geographic |
Argentina Toledo |
geographic_facet |
Argentina Toledo |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1039/C7CY02466E info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2018/cy/c7cy02466e http://hdl.handle.net/11336/83396 José, Carla; Toledo, Maria Victoria; Nicolás, Paula; Lasalle, Verónica; Ferreira, María Luján; et al.; Influence of the nature of the support on the catalytic performance of CALB: Experimental and theoretical evidence; Royal Society of Chemistry; Catalysis Science and Technology; 8; 14; 21-7-2018; 3513-3526 2044-4761 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1039/C7CY02466E |
container_title |
Catalysis Science & Technology |
container_volume |
8 |
container_issue |
14 |
container_start_page |
3513 |
op_container_end_page |
3526 |
_version_ |
1779317413344444416 |