Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts
Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent model systems for investigating micr...
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ftconicet:oai:ri.conicet.gov.ar:11336/79410 2023-10-09T21:46:30+02:00 Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts Le, Phuong Thi Makhalanyane, Thulani P. Guerrero, Leandro Demián Vikram, Surendra Van De Peer, Yves Cowan, Don A. application/pdf http://hdl.handle.net/11336/79410 eng eng Oxford University Press info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/gbe/article/8/9/2737/2236390 info:eu-repo/semantics/altIdentifier/doi/10.1093/gbe/evw189 http://hdl.handle.net/11336/79410 Le, Phuong Thi; Makhalanyane, Thulani P.; Guerrero, Leandro Demián; Vikram, Surendra; Van De Peer, Yves; et al.; Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts; Oxford University Press; Genome Biology and Evolution; 8; 9; 8-2016; 2737-2747 1759-6653 CONICET Digital CONICET info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ ANTARCTICA BIOMES COMPARATIVE METAGENOMICS DESERTS HYPOLITHS NAMIB DESERT SOILS STRESS RESPONSE https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1093/gbe/evw189 2023-09-24T18:24:43Z Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent model systems for investigating microbial responses to environmental stressors. However, relatively little is known about the genetic diversity underlying such functional processes in climatically extreme desert systems. This study presents the first comparative metagenome analysis of cyanobacteria-dominated hypolithic communities in hot (Namib Desert, Namibia) and cold (Miers Valley, Antarctica) hyperarid deserts. The most abundant phyla in both hypolith metagenomes were Actinobacteria, Proteobacteria, Cyanobacteria and Bacteroidetes with Cyanobacteria dominating in Antarctic hypoliths. However, no significant differences between the twometagenomeswere identified. The Antarctic hypolithicmetagenome displayed a high number of sequences assigned to sigma factors, replication,recombination andrepair, translation, ribosomal structure,andbiogenesis. In contrast, theNamibDesert metagenome showed a high abundance of sequences assigned to carbohydrate transport and metabolism. Metagenome data analysis also revealed significantdivergence inthe geneticdeterminantsof aminoacidandnucleotidemetabolismbetween these two metagenomes and those of soil from other polar deserts, hot deserts, and non-desert soils. Our results suggest extensive niche differentiation in hypolithic microbial communities from these two extreme environments and a high genetic capacity for survival under environmental extremes. Fil: Le, Phuong Thi. University of Pretoria; Sudáfrica. Vlaams Instituut voor Biotechnologie; Bélgica. University of Ghent; Bélgica Fil: Makhalanyane, Thulani P. University of Pretoria; Sudáfrica Fil: Guerrero, Leandro Demián. University of Pretoria; Sudáfrica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería ... Article in Journal/Newspaper Antarc* Antarctic Antarctica CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Antarctic The Antarctic Miers ENVELOPE(163.850,163.850,-78.100,-78.100) Miers Valley ENVELOPE(164.200,164.200,-78.100,-78.100) Genome Biology and Evolution 8 9 2737 2747 |
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CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
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language |
English |
topic |
ANTARCTICA BIOMES COMPARATIVE METAGENOMICS DESERTS HYPOLITHS NAMIB DESERT SOILS STRESS RESPONSE https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
spellingShingle |
ANTARCTICA BIOMES COMPARATIVE METAGENOMICS DESERTS HYPOLITHS NAMIB DESERT SOILS STRESS RESPONSE https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Le, Phuong Thi Makhalanyane, Thulani P. Guerrero, Leandro Demián Vikram, Surendra Van De Peer, Yves Cowan, Don A. Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
topic_facet |
ANTARCTICA BIOMES COMPARATIVE METAGENOMICS DESERTS HYPOLITHS NAMIB DESERT SOILS STRESS RESPONSE https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
description |
Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent model systems for investigating microbial responses to environmental stressors. However, relatively little is known about the genetic diversity underlying such functional processes in climatically extreme desert systems. This study presents the first comparative metagenome analysis of cyanobacteria-dominated hypolithic communities in hot (Namib Desert, Namibia) and cold (Miers Valley, Antarctica) hyperarid deserts. The most abundant phyla in both hypolith metagenomes were Actinobacteria, Proteobacteria, Cyanobacteria and Bacteroidetes with Cyanobacteria dominating in Antarctic hypoliths. However, no significant differences between the twometagenomeswere identified. The Antarctic hypolithicmetagenome displayed a high number of sequences assigned to sigma factors, replication,recombination andrepair, translation, ribosomal structure,andbiogenesis. In contrast, theNamibDesert metagenome showed a high abundance of sequences assigned to carbohydrate transport and metabolism. Metagenome data analysis also revealed significantdivergence inthe geneticdeterminantsof aminoacidandnucleotidemetabolismbetween these two metagenomes and those of soil from other polar deserts, hot deserts, and non-desert soils. Our results suggest extensive niche differentiation in hypolithic microbial communities from these two extreme environments and a high genetic capacity for survival under environmental extremes. Fil: Le, Phuong Thi. University of Pretoria; Sudáfrica. Vlaams Instituut voor Biotechnologie; Bélgica. University of Ghent; Bélgica Fil: Makhalanyane, Thulani P. University of Pretoria; Sudáfrica Fil: Guerrero, Leandro Demián. University of Pretoria; Sudáfrica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería ... |
format |
Article in Journal/Newspaper |
author |
Le, Phuong Thi Makhalanyane, Thulani P. Guerrero, Leandro Demián Vikram, Surendra Van De Peer, Yves Cowan, Don A. |
author_facet |
Le, Phuong Thi Makhalanyane, Thulani P. Guerrero, Leandro Demián Vikram, Surendra Van De Peer, Yves Cowan, Don A. |
author_sort |
Le, Phuong Thi |
title |
Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
title_short |
Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
title_full |
Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
title_fullStr |
Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
title_full_unstemmed |
Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
title_sort |
comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
publisher |
Oxford University Press |
url |
http://hdl.handle.net/11336/79410 |
long_lat |
ENVELOPE(163.850,163.850,-78.100,-78.100) ENVELOPE(164.200,164.200,-78.100,-78.100) |
geographic |
Antarctic The Antarctic Miers Miers Valley |
geographic_facet |
Antarctic The Antarctic Miers Miers Valley |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/gbe/article/8/9/2737/2236390 info:eu-repo/semantics/altIdentifier/doi/10.1093/gbe/evw189 http://hdl.handle.net/11336/79410 Le, Phuong Thi; Makhalanyane, Thulani P.; Guerrero, Leandro Demián; Vikram, Surendra; Van De Peer, Yves; et al.; Comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts; Oxford University Press; Genome Biology and Evolution; 8; 9; 8-2016; 2737-2747 1759-6653 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1093/gbe/evw189 |
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Genome Biology and Evolution |
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8 |
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9 |
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2737 |
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2747 |
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1779322206714593280 |