The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics
The dominant perciform suborder Notothenioidei is an excellent study group for assessing the evolution and functional importance of biochemical adaptations to temperature. The availability of notothenioid taxa in a wide range of latitudes (Antarctic and non-Antarctic) provides a tool to enable ident...
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ftconicet:oai:ri.conicet.gov.ar:11336/74803 2023-10-09T21:47:07+02:00 The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics Giordano, Daniela Boechi, Leonardo Vergara, Alessandro Marti, Marcelo Adrian Samuni, Uri Dantsker, David Grassi, Luigi Estrin, Dario Ariel Friedman, Joel M. Mazzarella, Lelio Di Prisco, Guido Verde, Cinzia application/pdf http://hdl.handle.net/11336/74803 eng eng Wiley Blackwell Publishing, Inc info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1742-4658.2009.06954.x info:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/full/10.1111/j.1742-4658.2009.06954.x http://hdl.handle.net/11336/74803 Giordano, Daniela; Boechi, Leonardo; Vergara, Alessandro; Marti, Marcelo Adrian; Samuni, Uri; et al.; The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics; Wiley Blackwell Publishing, Inc; Febs Journal; 276; 8; 4-2009; 2266-2277 1742-464X CONICET Digital CONICET info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ Antarctica Computer Simulation Hemoglobin Ligand-Binding Properties Oxygen Affinity https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1111/j.1742-4658.2009.06954.x 2023-09-24T18:26:04Z The dominant perciform suborder Notothenioidei is an excellent study group for assessing the evolution and functional importance of biochemical adaptations to temperature. The availability of notothenioid taxa in a wide range of latitudes (Antarctic and non-Antarctic) provides a tool to enable identification of physiological and biochemical characteristics gained and lost during evolutionary history. Non-Antarctic notothenioids belonging to the most basal families are a crucial source for understanding the evolution of hemoglobin in high-Antarctic cold-adapted fish. This paper focuses on the structure, function and evolution of the oxygen-transport system of Cottoperca gobio, a sub-Antarctic notothenioid fish of the family Bovichtidae, probably derived from ancestral species that evolved in the Antarctic region and later migrated to lower latitudes. Unlike most high-Antarctic notothenioids, but similar to many other acanthomorph teleosts, C. gobio has two major hemoglobins having the β chain in common. The oxygen-binding equilibria and kinetics of the two hemoglobins have been measured. Hb1 and Hb2 show strong modulation of oxygen-binding equilibria and kinetics by heterotropic effectors, with marked Bohr and Root effects. In Hb1 and Hb2, oxygen affinity and subunit cooperativity are slightly higher than in most high-Antarctic notothenioid hemoglobins. Hb1 and Hb2 show similar rebinding rates, but also show significant dynamic differences that are likely to have functional consequences. Molecular dynamic simulations of C. gobio Hb1 were performed on the dimeric protein in order to obtain a better understanding of the molecular basis of structure/function relationships. Fil: Giordano, Daniela. Consiglio Nazionale delle Ricerche; Italia Fil: Boechi, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y ... Article in Journal/Newspaper Antarc* Antarctic Antarctica CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Antarctic The Antarctic FEBS Journal 276 8 2266 2277 |
institution |
Open Polar |
collection |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
op_collection_id |
ftconicet |
language |
English |
topic |
Antarctica Computer Simulation Hemoglobin Ligand-Binding Properties Oxygen Affinity https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
spellingShingle |
Antarctica Computer Simulation Hemoglobin Ligand-Binding Properties Oxygen Affinity https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 Giordano, Daniela Boechi, Leonardo Vergara, Alessandro Marti, Marcelo Adrian Samuni, Uri Dantsker, David Grassi, Luigi Estrin, Dario Ariel Friedman, Joel M. Mazzarella, Lelio Di Prisco, Guido Verde, Cinzia The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics |
topic_facet |
Antarctica Computer Simulation Hemoglobin Ligand-Binding Properties Oxygen Affinity https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
description |
The dominant perciform suborder Notothenioidei is an excellent study group for assessing the evolution and functional importance of biochemical adaptations to temperature. The availability of notothenioid taxa in a wide range of latitudes (Antarctic and non-Antarctic) provides a tool to enable identification of physiological and biochemical characteristics gained and lost during evolutionary history. Non-Antarctic notothenioids belonging to the most basal families are a crucial source for understanding the evolution of hemoglobin in high-Antarctic cold-adapted fish. This paper focuses on the structure, function and evolution of the oxygen-transport system of Cottoperca gobio, a sub-Antarctic notothenioid fish of the family Bovichtidae, probably derived from ancestral species that evolved in the Antarctic region and later migrated to lower latitudes. Unlike most high-Antarctic notothenioids, but similar to many other acanthomorph teleosts, C. gobio has two major hemoglobins having the β chain in common. The oxygen-binding equilibria and kinetics of the two hemoglobins have been measured. Hb1 and Hb2 show strong modulation of oxygen-binding equilibria and kinetics by heterotropic effectors, with marked Bohr and Root effects. In Hb1 and Hb2, oxygen affinity and subunit cooperativity are slightly higher than in most high-Antarctic notothenioid hemoglobins. Hb1 and Hb2 show similar rebinding rates, but also show significant dynamic differences that are likely to have functional consequences. Molecular dynamic simulations of C. gobio Hb1 were performed on the dimeric protein in order to obtain a better understanding of the molecular basis of structure/function relationships. Fil: Giordano, Daniela. Consiglio Nazionale delle Ricerche; Italia Fil: Boechi, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y ... |
format |
Article in Journal/Newspaper |
author |
Giordano, Daniela Boechi, Leonardo Vergara, Alessandro Marti, Marcelo Adrian Samuni, Uri Dantsker, David Grassi, Luigi Estrin, Dario Ariel Friedman, Joel M. Mazzarella, Lelio Di Prisco, Guido Verde, Cinzia |
author_facet |
Giordano, Daniela Boechi, Leonardo Vergara, Alessandro Marti, Marcelo Adrian Samuni, Uri Dantsker, David Grassi, Luigi Estrin, Dario Ariel Friedman, Joel M. Mazzarella, Lelio Di Prisco, Guido Verde, Cinzia |
author_sort |
Giordano, Daniela |
title |
The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics |
title_short |
The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics |
title_full |
The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics |
title_fullStr |
The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics |
title_full_unstemmed |
The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics |
title_sort |
hemoglobins of the sub-antarctic fish cottoperca gobio, a phyletically basal species - oxygen-binding equilibria, kinetics and molecular dynamics |
publisher |
Wiley Blackwell Publishing, Inc |
url |
http://hdl.handle.net/11336/74803 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1742-4658.2009.06954.x info:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/full/10.1111/j.1742-4658.2009.06954.x http://hdl.handle.net/11336/74803 Giordano, Daniela; Boechi, Leonardo; Vergara, Alessandro; Marti, Marcelo Adrian; Samuni, Uri; et al.; The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics; Wiley Blackwell Publishing, Inc; Febs Journal; 276; 8; 4-2009; 2266-2277 1742-464X CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1111/j.1742-4658.2009.06954.x |
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FEBS Journal |
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276 |
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8 |
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2266 |
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2277 |
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1779309875343392768 |