Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach
Diesel fuel is one of the most important sources of hydrocarbon contamination worldwide. Its composition consists of a complex mixture of n-alkanes, branched alkanes and aromatic compounds. Hydrocarbon degradation in Pseudomonas species has been mostly studied under aerobic conditions; however, a dy...
Published in: | Journal of Industrial Microbiology and Biotechnology |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Springer Heidelberg
|
Subjects: | |
Online Access: | http://hdl.handle.net/11336/53953 |
id |
ftconicet:oai:ri.conicet.gov.ar:11336/53953 |
---|---|
record_format |
openpolar |
spelling |
ftconicet:oai:ri.conicet.gov.ar:11336/53953 2023-10-09T21:47:14+02:00 Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach Tribelli, Paula Maria Rossi, Leticia Ricardi, Martiniano María Gomez Lozano, Maria Molin, Søren Raiger Iustman, Laura Judith López, Nancy Irene application/pdf http://hdl.handle.net/11336/53953 eng eng Springer Heidelberg info:eu-repo/semantics/altIdentifier/doi/10.1007/s10295-017-1987-z info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10295-017-1987-z http://hdl.handle.net/11336/53953 1367-5435 1476-5535 CONICET Digital CONICET info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ ALKANE DEGRADATION ALKB MICRO-AEROBIOSIS PSEUDOMONAS EXTREMAUSTRALIS RNA-SEQ https://purl.org/becyt/ford/2.8 https://purl.org/becyt/ford/2 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1007/s10295-017-1987-z 2023-09-24T19:40:47Z Diesel fuel is one of the most important sources of hydrocarbon contamination worldwide. Its composition consists of a complex mixture of n-alkanes, branched alkanes and aromatic compounds. Hydrocarbon degradation in Pseudomonas species has been mostly studied under aerobic conditions; however, a dynamic spectrum of oxygen availability can be found in the environment. Pseudomonas extremaustralis, an Antarctic bacterium isolated from a pristine environment, is able to degrade diesel fuel and presents a wide microaerophilic metabolism. In this work RNA-deep sequence experiments were analyzed comparing the expression profile in aerobic and microaerophilic cultures. Interestingly, genes involved in alkane degradation, including alkB, were over-expressed in micro-aerobiosis in absence of hydrocarbon compounds. In minimal media supplemented with diesel fuel, n-alkanes degradation (C13–C19) after 7 days was observed under low oxygen conditions but not in aerobiosis. In-silico analysis of the alkB promoter zone showed a putative binding sequence for the anaerobic global regulator, Anr. Our results indicate that some diesel fuel components can be utilized as sole carbon source under microaerophilic conditions for cell maintenance or slow growth in a Pseudomonas species and this metabolism could represent an adaptive advantage in polluted environments. Fil: Tribelli, Paula Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Rossi, Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y ... Article in Journal/Newspaper Antarc* Antarctic CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Antarctic Argentina Journal of Industrial Microbiology and Biotechnology 45 1 15 23 |
institution |
Open Polar |
collection |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
op_collection_id |
ftconicet |
language |
English |
topic |
ALKANE DEGRADATION ALKB MICRO-AEROBIOSIS PSEUDOMONAS EXTREMAUSTRALIS RNA-SEQ https://purl.org/becyt/ford/2.8 https://purl.org/becyt/ford/2 |
spellingShingle |
ALKANE DEGRADATION ALKB MICRO-AEROBIOSIS PSEUDOMONAS EXTREMAUSTRALIS RNA-SEQ https://purl.org/becyt/ford/2.8 https://purl.org/becyt/ford/2 Tribelli, Paula Maria Rossi, Leticia Ricardi, Martiniano María Gomez Lozano, Maria Molin, Søren Raiger Iustman, Laura Judith López, Nancy Irene Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
topic_facet |
ALKANE DEGRADATION ALKB MICRO-AEROBIOSIS PSEUDOMONAS EXTREMAUSTRALIS RNA-SEQ https://purl.org/becyt/ford/2.8 https://purl.org/becyt/ford/2 |
description |
Diesel fuel is one of the most important sources of hydrocarbon contamination worldwide. Its composition consists of a complex mixture of n-alkanes, branched alkanes and aromatic compounds. Hydrocarbon degradation in Pseudomonas species has been mostly studied under aerobic conditions; however, a dynamic spectrum of oxygen availability can be found in the environment. Pseudomonas extremaustralis, an Antarctic bacterium isolated from a pristine environment, is able to degrade diesel fuel and presents a wide microaerophilic metabolism. In this work RNA-deep sequence experiments were analyzed comparing the expression profile in aerobic and microaerophilic cultures. Interestingly, genes involved in alkane degradation, including alkB, were over-expressed in micro-aerobiosis in absence of hydrocarbon compounds. In minimal media supplemented with diesel fuel, n-alkanes degradation (C13–C19) after 7 days was observed under low oxygen conditions but not in aerobiosis. In-silico analysis of the alkB promoter zone showed a putative binding sequence for the anaerobic global regulator, Anr. Our results indicate that some diesel fuel components can be utilized as sole carbon source under microaerophilic conditions for cell maintenance or slow growth in a Pseudomonas species and this metabolism could represent an adaptive advantage in polluted environments. Fil: Tribelli, Paula Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Rossi, Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y ... |
format |
Article in Journal/Newspaper |
author |
Tribelli, Paula Maria Rossi, Leticia Ricardi, Martiniano María Gomez Lozano, Maria Molin, Søren Raiger Iustman, Laura Judith López, Nancy Irene |
author_facet |
Tribelli, Paula Maria Rossi, Leticia Ricardi, Martiniano María Gomez Lozano, Maria Molin, Søren Raiger Iustman, Laura Judith López, Nancy Irene |
author_sort |
Tribelli, Paula Maria |
title |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
title_short |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
title_full |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
title_fullStr |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
title_full_unstemmed |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
title_sort |
microaerophilic alkane degradation in pseudomonas extremaustralis: a transcriptomic and physiological approach |
publisher |
Springer Heidelberg |
url |
http://hdl.handle.net/11336/53953 |
geographic |
Antarctic Argentina |
geographic_facet |
Antarctic Argentina |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10295-017-1987-z info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10295-017-1987-z http://hdl.handle.net/11336/53953 1367-5435 1476-5535 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1007/s10295-017-1987-z |
container_title |
Journal of Industrial Microbiology and Biotechnology |
container_volume |
45 |
container_issue |
1 |
container_start_page |
15 |
op_container_end_page |
23 |
_version_ |
1779310194638979072 |