Molecular recognition of an acyl–enzyme intermediate on the lipase B from Candida antarctica
This investigation provides evidence of the acyl enzyme species involved in the interaction of R/S-ketoprofen with the lipase B from Candida antarctica. The interaction between the profen and the enzyme was studied by in situ time-resolved ATR-FTIR under both static and transient conditions. Particu...
| Published in: | Catalysis Science & Technology |
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| Main Authors: | , , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Royal Society of Chemistry
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| Subjects: | |
| Online Access: | http://hdl.handle.net/11336/43265 |
| _version_ | 1821573440478380032 |
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| author | Toledo, Victoria Llerena Suster, Carlos Rafael Ferreira, María Luján Collins, Sebastián Enrique Briand, Laura Estefania |
| author_facet | Toledo, Victoria Llerena Suster, Carlos Rafael Ferreira, María Luján Collins, Sebastián Enrique Briand, Laura Estefania |
| author_sort | Toledo, Victoria |
| collection | CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| container_issue | 9 |
| container_start_page | 1953 |
| container_title | Catalysis Science & Technology |
| container_volume | 7 |
| description | This investigation provides evidence of the acyl enzyme species involved in the interaction of R/S-ketoprofen with the lipase B from Candida antarctica. The interaction between the profen and the enzyme was studied by in situ time-resolved ATR-FTIR under both static and transient conditions. Particularly, modulation excitation spectroscopy (MES) with phase sensitive detection (PSD) allowed us to univocally distinguish the signals belonging to the interaction between ketoprofen and the enzyme from the strong background signals. These experimental tools coupled with theoretical DFT analysis allowed us to propose various species derived from the interaction of ketoprofen with serine through H bonding (without reaction) and the acyl enzyme species (ester bond formation) which are the intermediates in the biocatalytic assisted esterification and hydrolysis using lipases. Fil: Toledo, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas Argentina Fil: Llerena Suster, Carlos Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas Argentina Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina Fil: Collins, Sebastián Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina Fil: Briand, Laura Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y ... |
| format | Article in Journal/Newspaper |
| genre | Antarc* Antarctica |
| genre_facet | Antarc* Antarctica |
| geographic | Argentina Briand Ferreira Toledo |
| geographic_facet | Argentina Briand Ferreira Toledo |
| id | ftconicet:oai:ri.conicet.gov.ar:11336/43265 |
| institution | Open Polar |
| language | English |
| long_lat | ENVELOPE(-63.017,-63.017,-65.017,-65.017) ENVELOPE(-62.050,-62.050,-64.600,-64.600) ENVELOPE(-67.317,-67.317,-73.700,-73.700) |
| op_collection_id | ftconicet |
| op_container_end_page | 1964 |
| op_doi | https://doi.org/10.1039/C7CY00245A |
| op_relation | info:eu-repo/semantics/altIdentifier/doi/10.1039/C7CY00245A info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2017/CY/C7CY00245A http://hdl.handle.net/11336/43265 Toledo, Victoria; Llerena Suster, Carlos Rafael; Ferreira, María Luján; Collins, Sebastián Enrique; Briand, Laura Estefania; Molecular recognition of an acyl–enzyme intermediate on the lipase B from Candida antarctica; Royal Society of Chemistry; Catalysis Science & Technology; 7; 9; 11-2017; 1953-1964 2044-4761 CONICET Digital CONICET |
| op_rights | info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
| publisher | Royal Society of Chemistry |
| record_format | openpolar |
| spelling | ftconicet:oai:ri.conicet.gov.ar:11336/43265 2025-01-16T19:06:29+00:00 Molecular recognition of an acyl–enzyme intermediate on the lipase B from Candida antarctica Toledo, Victoria Llerena Suster, Carlos Rafael Ferreira, María Luján Collins, Sebastián Enrique Briand, Laura Estefania application/pdf http://hdl.handle.net/11336/43265 eng eng Royal Society of Chemistry info:eu-repo/semantics/altIdentifier/doi/10.1039/C7CY00245A info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2017/CY/C7CY00245A http://hdl.handle.net/11336/43265 Toledo, Victoria; Llerena Suster, Carlos Rafael; Ferreira, María Luján; Collins, Sebastián Enrique; Briand, Laura Estefania; Molecular recognition of an acyl–enzyme intermediate on the lipase B from Candida antarctica; Royal Society of Chemistry; Catalysis Science & Technology; 7; 9; 11-2017; 1953-1964 2044-4761 CONICET Digital CONICET info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ Ketoprofen Serine https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1039/C7CY00245A 2023-09-24T20:04:08Z This investigation provides evidence of the acyl enzyme species involved in the interaction of R/S-ketoprofen with the lipase B from Candida antarctica. The interaction between the profen and the enzyme was studied by in situ time-resolved ATR-FTIR under both static and transient conditions. Particularly, modulation excitation spectroscopy (MES) with phase sensitive detection (PSD) allowed us to univocally distinguish the signals belonging to the interaction between ketoprofen and the enzyme from the strong background signals. These experimental tools coupled with theoretical DFT analysis allowed us to propose various species derived from the interaction of ketoprofen with serine through H bonding (without reaction) and the acyl enzyme species (ester bond formation) which are the intermediates in the biocatalytic assisted esterification and hydrolysis using lipases. Fil: Toledo, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas Argentina Fil: Llerena Suster, Carlos Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas Argentina Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina Fil: Collins, Sebastián Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina Fil: Briand, Laura Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y ... Article in Journal/Newspaper Antarc* Antarctica CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Argentina Briand ENVELOPE(-63.017,-63.017,-65.017,-65.017) Ferreira ENVELOPE(-62.050,-62.050,-64.600,-64.600) Toledo ENVELOPE(-67.317,-67.317,-73.700,-73.700) Catalysis Science & Technology 7 9 1953 1964 |
| spellingShingle | Ketoprofen Serine https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 Toledo, Victoria Llerena Suster, Carlos Rafael Ferreira, María Luján Collins, Sebastián Enrique Briand, Laura Estefania Molecular recognition of an acyl–enzyme intermediate on the lipase B from Candida antarctica |
| title | Molecular recognition of an acyl–enzyme intermediate on the lipase B from Candida antarctica |
| title_full | Molecular recognition of an acyl–enzyme intermediate on the lipase B from Candida antarctica |
| title_fullStr | Molecular recognition of an acyl–enzyme intermediate on the lipase B from Candida antarctica |
| title_full_unstemmed | Molecular recognition of an acyl–enzyme intermediate on the lipase B from Candida antarctica |
| title_short | Molecular recognition of an acyl–enzyme intermediate on the lipase B from Candida antarctica |
| title_sort | molecular recognition of an acyl–enzyme intermediate on the lipase b from candida antarctica |
| topic | Ketoprofen Serine https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
| topic_facet | Ketoprofen Serine https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
| url | http://hdl.handle.net/11336/43265 |