Kinetic analysis of enzymatic esterification of fatty acids and ethanol

• Main Authors: Borio, Daniel Oscar, Trubiano, G.C., Perez, Guillermo Agustin, Errazu, Alberto Felipe
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier
• Subjects: Kinetics; Fatty-Acids; Esterification; Enzimatic; https://purl.org/becyt/ford/2.4; https://purl.org/becyt/ford/2; Argentina; Perez; antartic*
• Online Access: http://hdl.handle.net/11336/40088

The esterification of oleic acid and ethanol with immobilised lipase in a solvent-free media is presented in this work. The reaction has been carried out at a laboratory scale in a stirred tank reactor operating batchwise. The evolution of oleic acid conversion to ester was determined by using two analysis techniques: measurement of the acid index and gas chromatography. The influence of the operating conditions on the equilibrium conversion and conversion-time profiles was analysed. The amount of enzyme, temperature, initial alcohol/oleic acid molar ratio and initial water content were considered. A kinetic model has been developed for the enzymatic esterification of oleic acid and ethanol catalysed by Candida Antartica immobilised lipase. Some kinetic models were considered and a second order reaction kinetic was selected. The parameter adjustment was carried out by using a computer program based on Marquardt algorithms. The results from the simulated experiments by using the proposed model showed a satisfactory agreement with the experimental data. Fil: Borio, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina Fil: Trubiano, G.C. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina Fil: Perez, Guillermo Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina Fil: Borio, Daniel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. ...