Antioxidant levels from different Antarctic fish caught around South Georgia Island and Shag Rocks
Antarctic fish have been isolated for over several million years in an environment with a very low and constant temperature and high oxygen concentration. In such conditions the oxidative stress might be an important factor affecting their metabolic adaptive strategies. Activity of the antioxidant e...
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ftconicet:oai:ri.conicet.gov.ar:11336/39199 2023-10-09T21:44:58+02:00 Antioxidant levels from different Antarctic fish caught around South Georgia Island and Shag Rocks Ansaldo, Martin Luquet, Carlos Marcelo Evelson, Pablo Andrés Polo, José M. Llesuy, Susana Francisca application/pdf http://hdl.handle.net/11336/39199 eng eng Springer info:eu-repo/semantics/altIdentifier/doi/10.1007/s003000050022 info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s003000050022 http://hdl.handle.net/11336/39199 Ansaldo, Martin; Luquet, Carlos Marcelo; Evelson, Pablo Andrés; Polo, José M.; Llesuy, Susana Francisca; Antioxidant levels from different Antarctic fish caught around South Georgia Island and Shag Rocks; Springer; Polar Biology; 23; 3; 2-2000; 160-165 0722-4060 CONICET Digital CONICET info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ Antartic Fish Antioxidants Total Reactive Antioxidant Potential https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1007/s003000050022 2023-09-24T18:54:50Z Antarctic fish have been isolated for over several million years in an environment with a very low and constant temperature and high oxygen concentration. In such conditions the oxidative stress might be an important factor affecting their metabolic adaptive strategies. Activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), vitamin E levels and total antioxidant capacity (TRAP) were measured in liver, gill, heart and muscle homogenates of red-blooded (Nototheniidae) and white-blooded (Channichthyidae) Antarctic fish. SOD activity was also measured in blood samples. Gill SOD activity was threefold higher in channichthyids than in nototheniids while CAT and GPx were significantly higher in the gills of channichthyids. The increased SOD activity of channichthyids probably reflects the large PO2 gradient across their gills. The H2O2 produced seems to be preferentially eliminated by diffusion, according to the low levels of CAT and GPx found in the gills of these species. In contrast, blood SOD was about fivefold higher in the latter group, which possesses erythrocytes and thus a much higher oxygen-carrying capacity. CAT activity was always higher in nototheniids except in muscle. However, vitamin E did not show clear differences between families except for the pattern observed in muscle. The higher content of vitamin E in this tissue shown in channichthyids is related to the higher volume density of mitochondria reported for this group, since vitamin E is responsible for preventing membrane lipid peroxidation. Accordingly, TRAP (representative of hydrosoluble antioxidant capacity) was also higher in muscle of channichthyids. This is probably related to the role of ascorbic (a hydrosoluble compound) acid in regenerating vitamin E. Fil: Ansaldo, Martin. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina Fil: Luquet, Carlos Marcelo. Universidad de Buenos Aires. Facultad de ... Article in Journal/Newspaper Antarc* Antarctic antartic* Dirección Nacional del Antártico Instituto Antártico Argentino Polar Biology South Georgia Island CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Antarctic Argentina Argentino Shag Rocks ENVELOPE(-42.033,-42.033,-53.550,-53.550) South Georgia Island ENVELOPE(-36.750,-36.750,-54.250,-54.250) Polar Biology 23 3 160 165 |
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Open Polar |
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CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
op_collection_id |
ftconicet |
language |
English |
topic |
Antartic Fish Antioxidants Total Reactive Antioxidant Potential https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
spellingShingle |
Antartic Fish Antioxidants Total Reactive Antioxidant Potential https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Ansaldo, Martin Luquet, Carlos Marcelo Evelson, Pablo Andrés Polo, José M. Llesuy, Susana Francisca Antioxidant levels from different Antarctic fish caught around South Georgia Island and Shag Rocks |
topic_facet |
Antartic Fish Antioxidants Total Reactive Antioxidant Potential https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
description |
Antarctic fish have been isolated for over several million years in an environment with a very low and constant temperature and high oxygen concentration. In such conditions the oxidative stress might be an important factor affecting their metabolic adaptive strategies. Activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), vitamin E levels and total antioxidant capacity (TRAP) were measured in liver, gill, heart and muscle homogenates of red-blooded (Nototheniidae) and white-blooded (Channichthyidae) Antarctic fish. SOD activity was also measured in blood samples. Gill SOD activity was threefold higher in channichthyids than in nototheniids while CAT and GPx were significantly higher in the gills of channichthyids. The increased SOD activity of channichthyids probably reflects the large PO2 gradient across their gills. The H2O2 produced seems to be preferentially eliminated by diffusion, according to the low levels of CAT and GPx found in the gills of these species. In contrast, blood SOD was about fivefold higher in the latter group, which possesses erythrocytes and thus a much higher oxygen-carrying capacity. CAT activity was always higher in nototheniids except in muscle. However, vitamin E did not show clear differences between families except for the pattern observed in muscle. The higher content of vitamin E in this tissue shown in channichthyids is related to the higher volume density of mitochondria reported for this group, since vitamin E is responsible for preventing membrane lipid peroxidation. Accordingly, TRAP (representative of hydrosoluble antioxidant capacity) was also higher in muscle of channichthyids. This is probably related to the role of ascorbic (a hydrosoluble compound) acid in regenerating vitamin E. Fil: Ansaldo, Martin. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina Fil: Luquet, Carlos Marcelo. Universidad de Buenos Aires. Facultad de ... |
format |
Article in Journal/Newspaper |
author |
Ansaldo, Martin Luquet, Carlos Marcelo Evelson, Pablo Andrés Polo, José M. Llesuy, Susana Francisca |
author_facet |
Ansaldo, Martin Luquet, Carlos Marcelo Evelson, Pablo Andrés Polo, José M. Llesuy, Susana Francisca |
author_sort |
Ansaldo, Martin |
title |
Antioxidant levels from different Antarctic fish caught around South Georgia Island and Shag Rocks |
title_short |
Antioxidant levels from different Antarctic fish caught around South Georgia Island and Shag Rocks |
title_full |
Antioxidant levels from different Antarctic fish caught around South Georgia Island and Shag Rocks |
title_fullStr |
Antioxidant levels from different Antarctic fish caught around South Georgia Island and Shag Rocks |
title_full_unstemmed |
Antioxidant levels from different Antarctic fish caught around South Georgia Island and Shag Rocks |
title_sort |
antioxidant levels from different antarctic fish caught around south georgia island and shag rocks |
publisher |
Springer |
url |
http://hdl.handle.net/11336/39199 |
long_lat |
ENVELOPE(-42.033,-42.033,-53.550,-53.550) ENVELOPE(-36.750,-36.750,-54.250,-54.250) |
geographic |
Antarctic Argentina Argentino Shag Rocks South Georgia Island |
geographic_facet |
Antarctic Argentina Argentino Shag Rocks South Georgia Island |
genre |
Antarc* Antarctic antartic* Dirección Nacional del Antártico Instituto Antártico Argentino Polar Biology South Georgia Island |
genre_facet |
Antarc* Antarctic antartic* Dirección Nacional del Antártico Instituto Antártico Argentino Polar Biology South Georgia Island |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s003000050022 info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s003000050022 http://hdl.handle.net/11336/39199 Ansaldo, Martin; Luquet, Carlos Marcelo; Evelson, Pablo Andrés; Polo, José M.; Llesuy, Susana Francisca; Antioxidant levels from different Antarctic fish caught around South Georgia Island and Shag Rocks; Springer; Polar Biology; 23; 3; 2-2000; 160-165 0722-4060 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1007/s003000050022 |
container_title |
Polar Biology |
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23 |
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3 |
container_start_page |
160 |
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165 |
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1779315012165173248 |