Reversible immobilization of lipases on octyl-glutamic agarose beads: a mixed adsorption that reinforces enzyme immobilization

• Published in: Journal of Molecular Catalysis B: Enzymatic
• Main Authors: Rueda, Nazzoly, Dos Santos, Cleiton S., Rodríguez, María Daniela, Albuquerque, Tiago L., Barbosa, Oveimar, Torres, Rodrigo, Ortiz, Claudia, Fernandez Lafuente, Roberto
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier Science
• Subjects: ENZYME STABILIZATION; HETEROFUNCTIONAL SUPPORTS; ION EXCHANGE; LIPASE INTERFACIAL ACTIVATION; PREVENTION OF ENZYME LEAKAGE; REVERSIBLE IMMOBILIZATION; https://purl.org/becyt/ford/2.9; https://purl.org/becyt/ford/2; Argentina; Misiones; Rugosa; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/11336/39050

A new octyl-glutamic(OCGLU) heterofunctional agarose bead has been prepared. It has been compared to octyl-agarose (OC) in their performance to immobilize 5 different lipases, those from Candida antarctica (A (CALA) and B (CALB)), from Thermomyces lanuginosus (TLL), from Rhizomucor miehei (RML) and from Candida rugosa (CRL) and a phospholipase (Lecitase ultra, LU). The immobilization rate was very similar using both supports, and the increase of activity versus p-nitrophenyl butyrate were also very similar. The effects on enzyme stability of the immobilization on OCGLU compared to the conventional OC was quite diverse, in some cases reducing the enzyme stability while in other examples the enzyme stability improved more than hundredfold. Curiously, the highest stabilizations were found under pH conditions where the free enzyme could not be adsorbed on a support just bearing glutamic groups on its surface, suggesting that the mechanism of stabilization may be a quite complex one that should consider the hydrophilization of the support surface, the cationic and anionic groups of glutamic, the likely partition of organic solvents from the support surface, positive and negative enzyme-support interactions, etc. Even though the lipases adsorption was very strong, the support could be regenerated and reused by incubation in ionic detergents. Fil: Rueda, Nazzoly. Universidad Industrial Santander; Colombia. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España Fil: Dos Santos, Cleiton S. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España. Universidade Federal do Ceará; Brasil Fil: Rodríguez, María Daniela. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España Fil: Albuquerque, Tiago L. Consejo Superior de ...