Pseudomonas yamanorum sp. nov., a psychrotolerant bacterium isolated from subantarctic environment
A psychrotolerant strain, 8H1(T), was isolated from soil samples collected in Isla de los Estados, Ushuaia, Argentina. Cells were Gram-negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by means of two polar flagella. The isolate was able to grow in the rang...
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ftconicet:oai:ri.conicet.gov.ar:11336/30500 2023-05-15T13:56:51+02:00 Pseudomonas yamanorum sp. nov., a psychrotolerant bacterium isolated from subantarctic environment Arnau, Victor Gonzalo Sanchez, Leandro Arturo Delgado, Osvaldo Daniel application/pdf http://hdl.handle.net/11336/30500 eng eng Society for General Microbiology info:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.065201-0 info:eu-repo/semantics/altIdentifier/url/http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.065201-0 Arnau, Victor Gonzalo; Sanchez, Leandro Arturo; Delgado, Osvaldo Daniel; Pseudomonas yamanorum sp. nov., a psychrotolerant bacterium isolated from subantarctic environment; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 65; 2-2015; 424-431 1466-5026 http://hdl.handle.net/11336/30500 CONICET Digital CONICET info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ CC-BY-NC-SA Pseudomonas Taxonomy Tierra del Fuego Sub-Antarctic Otras Ciencias Biológicas Ciencias Biológicas CIENCIAS NATURALES Y EXACTAS info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1099/ijs.0.065201-0 2019-08-31T16:50:36Z A psychrotolerant strain, 8H1(T), was isolated from soil samples collected in Isla de los Estados, Ushuaia, Argentina. Cells were Gram-negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by means of two polar flagella. The isolate was able to grow in the range 4-35 °C, with optimum growth at 28 °C. The predominant cellular fatty acids were summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0 and summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). The polar lipid pattern of strain 8H1(T) comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. The DNA G+C content was 59.8 mol%. 16S rRNA gene sequence-based phylogeny suggested the affiliation of strain 8H1(T) to the ´Pseudomonas fluorescens group´, displaying ≥98.5 % sequence similarity to 29 type strains. A multilocus sequence analysis (MLSA) study performed by concatenating 16S rRNA, gyrB, rpoD and rpoB gene sequences showed that isolate 8H1(T) could be discriminated from closely related species of the genus Pseudomonas and placed in the ´Pseudomonas gessardii subgroup´, including the species with the highest MLSA sequence similarities: Pseudomonas brenneri (96.2 %), P. gessardii (96.1 %), P. proteolytica (96.0 %), P. meridiana (96.0 %) and P. mucidolens (95.4 %). DNA-DNA hybridization analysis between 8H1(T) and the type strains of these closely related species revealed relatedness values of 27.0, 8.8, 41.2, 39.7 and 46.1 %, respectively. These results, together with differences in several phenotypic features, support the classification of a novel species, for which the name Pseudomonas yamanorum sp. nov. is proposed. The type strain is 8H1(T) ( = DSM 26522(T) = CCUG 63249(T) = LMG 27247(T)). Fil: Arnau, Victor Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina Fil: Sanchez, Leandro Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina Article in Journal/Newspaper Antarc* Antarctic Tierra del Fuego CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Antarctic Argentina Ushuaia ENVELOPE(-40.000,-40.000,-82.167,-82.167) International Journal of Systematic and Evolutionary Microbiology 65 Pt_2 424 431 |
institution |
Open Polar |
collection |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
op_collection_id |
ftconicet |
language |
English |
topic |
Pseudomonas Taxonomy Tierra del Fuego Sub-Antarctic Otras Ciencias Biológicas Ciencias Biológicas CIENCIAS NATURALES Y EXACTAS |
spellingShingle |
Pseudomonas Taxonomy Tierra del Fuego Sub-Antarctic Otras Ciencias Biológicas Ciencias Biológicas CIENCIAS NATURALES Y EXACTAS Arnau, Victor Gonzalo Sanchez, Leandro Arturo Delgado, Osvaldo Daniel Pseudomonas yamanorum sp. nov., a psychrotolerant bacterium isolated from subantarctic environment |
topic_facet |
Pseudomonas Taxonomy Tierra del Fuego Sub-Antarctic Otras Ciencias Biológicas Ciencias Biológicas CIENCIAS NATURALES Y EXACTAS |
description |
A psychrotolerant strain, 8H1(T), was isolated from soil samples collected in Isla de los Estados, Ushuaia, Argentina. Cells were Gram-negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by means of two polar flagella. The isolate was able to grow in the range 4-35 °C, with optimum growth at 28 °C. The predominant cellular fatty acids were summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0 and summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). The polar lipid pattern of strain 8H1(T) comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. The DNA G+C content was 59.8 mol%. 16S rRNA gene sequence-based phylogeny suggested the affiliation of strain 8H1(T) to the ´Pseudomonas fluorescens group´, displaying ≥98.5 % sequence similarity to 29 type strains. A multilocus sequence analysis (MLSA) study performed by concatenating 16S rRNA, gyrB, rpoD and rpoB gene sequences showed that isolate 8H1(T) could be discriminated from closely related species of the genus Pseudomonas and placed in the ´Pseudomonas gessardii subgroup´, including the species with the highest MLSA sequence similarities: Pseudomonas brenneri (96.2 %), P. gessardii (96.1 %), P. proteolytica (96.0 %), P. meridiana (96.0 %) and P. mucidolens (95.4 %). DNA-DNA hybridization analysis between 8H1(T) and the type strains of these closely related species revealed relatedness values of 27.0, 8.8, 41.2, 39.7 and 46.1 %, respectively. These results, together with differences in several phenotypic features, support the classification of a novel species, for which the name Pseudomonas yamanorum sp. nov. is proposed. The type strain is 8H1(T) ( = DSM 26522(T) = CCUG 63249(T) = LMG 27247(T)). Fil: Arnau, Victor Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina Fil: Sanchez, Leandro Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina |
format |
Article in Journal/Newspaper |
author |
Arnau, Victor Gonzalo Sanchez, Leandro Arturo Delgado, Osvaldo Daniel |
author_facet |
Arnau, Victor Gonzalo Sanchez, Leandro Arturo Delgado, Osvaldo Daniel |
author_sort |
Arnau, Victor Gonzalo |
title |
Pseudomonas yamanorum sp. nov., a psychrotolerant bacterium isolated from subantarctic environment |
title_short |
Pseudomonas yamanorum sp. nov., a psychrotolerant bacterium isolated from subantarctic environment |
title_full |
Pseudomonas yamanorum sp. nov., a psychrotolerant bacterium isolated from subantarctic environment |
title_fullStr |
Pseudomonas yamanorum sp. nov., a psychrotolerant bacterium isolated from subantarctic environment |
title_full_unstemmed |
Pseudomonas yamanorum sp. nov., a psychrotolerant bacterium isolated from subantarctic environment |
title_sort |
pseudomonas yamanorum sp. nov., a psychrotolerant bacterium isolated from subantarctic environment |
publisher |
Society for General Microbiology |
url |
http://hdl.handle.net/11336/30500 |
long_lat |
ENVELOPE(-40.000,-40.000,-82.167,-82.167) |
geographic |
Antarctic Argentina Ushuaia |
geographic_facet |
Antarctic Argentina Ushuaia |
genre |
Antarc* Antarctic Tierra del Fuego |
genre_facet |
Antarc* Antarctic Tierra del Fuego |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.065201-0 info:eu-repo/semantics/altIdentifier/url/http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.065201-0 Arnau, Victor Gonzalo; Sanchez, Leandro Arturo; Delgado, Osvaldo Daniel; Pseudomonas yamanorum sp. nov., a psychrotolerant bacterium isolated from subantarctic environment; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 65; 2-2015; 424-431 1466-5026 http://hdl.handle.net/11336/30500 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_rightsnorm |
CC-BY-NC-SA |
op_doi |
https://doi.org/10.1099/ijs.0.065201-0 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
65 |
container_issue |
Pt_2 |
container_start_page |
424 |
op_container_end_page |
431 |
_version_ |
1766264441148538880 |