Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic
Cold−adapted marine bacteria producing extracellular hydrolytic enzymes are important for their industrial application and play a key role in degradation of particulate organic matter in their natural environment. In this work, members of a previously−obtained protease−producing bacterial collection...
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ftconicet:oai:ri.conicet.gov.ar:11336/2746 2023-10-09T21:46:04+02:00 Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic Tropeano, Mauro Vazquez, Susana Claudia Coria, Silvia Turjanski, Adrian Cicero, Daniel Bercovich, Andrés Cormack, Mac application/pdf http://hdl.handle.net/11336/2746 eng eng De Gruyter Open info:eu-repo/semantics/altIdentifier/url/http://www.degruyter.com/view/j/popore.2013.34.issue-3/popore-2013-0014/popore-2013-0014.xml http://hdl.handle.net/11336/2746 Tropeano, Mauro; Vazquez, Susana Claudia; Coria, Silvia; Turjanski, Adrian; Cicero, Daniel; et al.; Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic; De Gruyter Open; Polish Polar Research; 34; 3; 10-2013; 253-267 0138-0338 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Bioinformatica Anataritda Genomica Enzimas https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet 2023-09-24T19:05:43Z Cold−adapted marine bacteria producing extracellular hydrolytic enzymes are important for their industrial application and play a key role in degradation of particulate organic matter in their natural environment. In this work, members of a previously−obtained protease−producing bacterial collection isolated from different marine sources from Potter Cove (King George Island, South Shetlands) were taxonomically identified and screened for their ability to produce other economically relevant enzymes. Eighty−eight proteolytic bacterial isolates were grouped into 25 phylotypes based on their Amplified Ribosomal DNA Restriction Analysis profiles. The sequencing of the 16S rRNA genes from representative isolates of the phylotypes showed that the predominant culturable protease−producing bacteria belonged to the class Gammaproteobacteria and were affiliated to the genera Pseudomonas, Shewanella, Colwellia, and Pseudoalteromonas, the latter being the predominant group (64% of isolates). In addition, members of the classes Actinobacteria, Bacilli and Flavobacteria were found. Among the 88 isolates screened we detected producers of amylases (21), pectinases (67), cellulases (53), CM−cellulases (68), xylanases (55) and agarases (57). More than 85% of the isolates showed at least one of the extracellular enzymatic activities tested, with some of them producing up to six extracellular enzymes. Our results confirmed that using selective conditions to isolate producers of one extracellular enzyme activity increases the probability of recovering bacteria that will also produce additional extracellular enzymes. This finding establishes a starting point for future programs oriented to the prospecting for biomolecules in Antarctica. Fil: Tropeano, Mauro. No especifíca; Fil: Vazquez, Susana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Coria, Silvia. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. ... Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Polar Research CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Antarctic Argentina Coria ENVELOPE(-61.883,-61.883,-64.017,-64.017) King George Island Potter Cove Silvia ENVELOPE(-57.900,-57.900,-63.300,-63.300) The Antarctic Vazquez ENVELOPE(-64.000,-64.000,-65.433,-65.433) Polish Polar Research 34 3 253 267 |
institution |
Open Polar |
collection |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
op_collection_id |
ftconicet |
language |
English |
topic |
Bioinformatica Anataritda Genomica Enzimas https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
spellingShingle |
Bioinformatica Anataritda Genomica Enzimas https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Tropeano, Mauro Vazquez, Susana Claudia Coria, Silvia Turjanski, Adrian Cicero, Daniel Bercovich, Andrés Cormack, Mac Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic |
topic_facet |
Bioinformatica Anataritda Genomica Enzimas https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
description |
Cold−adapted marine bacteria producing extracellular hydrolytic enzymes are important for their industrial application and play a key role in degradation of particulate organic matter in their natural environment. In this work, members of a previously−obtained protease−producing bacterial collection isolated from different marine sources from Potter Cove (King George Island, South Shetlands) were taxonomically identified and screened for their ability to produce other economically relevant enzymes. Eighty−eight proteolytic bacterial isolates were grouped into 25 phylotypes based on their Amplified Ribosomal DNA Restriction Analysis profiles. The sequencing of the 16S rRNA genes from representative isolates of the phylotypes showed that the predominant culturable protease−producing bacteria belonged to the class Gammaproteobacteria and were affiliated to the genera Pseudomonas, Shewanella, Colwellia, and Pseudoalteromonas, the latter being the predominant group (64% of isolates). In addition, members of the classes Actinobacteria, Bacilli and Flavobacteria were found. Among the 88 isolates screened we detected producers of amylases (21), pectinases (67), cellulases (53), CM−cellulases (68), xylanases (55) and agarases (57). More than 85% of the isolates showed at least one of the extracellular enzymatic activities tested, with some of them producing up to six extracellular enzymes. Our results confirmed that using selective conditions to isolate producers of one extracellular enzyme activity increases the probability of recovering bacteria that will also produce additional extracellular enzymes. This finding establishes a starting point for future programs oriented to the prospecting for biomolecules in Antarctica. Fil: Tropeano, Mauro. No especifíca; Fil: Vazquez, Susana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Coria, Silvia. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. ... |
format |
Article in Journal/Newspaper |
author |
Tropeano, Mauro Vazquez, Susana Claudia Coria, Silvia Turjanski, Adrian Cicero, Daniel Bercovich, Andrés Cormack, Mac |
author_facet |
Tropeano, Mauro Vazquez, Susana Claudia Coria, Silvia Turjanski, Adrian Cicero, Daniel Bercovich, Andrés Cormack, Mac |
author_sort |
Tropeano, Mauro |
title |
Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic |
title_short |
Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic |
title_full |
Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic |
title_fullStr |
Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic |
title_full_unstemmed |
Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic |
title_sort |
extracellular hydrolytic enzyme production by proteolytic bacteria from the antarctic |
publisher |
De Gruyter Open |
url |
http://hdl.handle.net/11336/2746 |
long_lat |
ENVELOPE(-61.883,-61.883,-64.017,-64.017) ENVELOPE(-57.900,-57.900,-63.300,-63.300) ENVELOPE(-64.000,-64.000,-65.433,-65.433) |
geographic |
Antarctic Argentina Coria King George Island Potter Cove Silvia The Antarctic Vazquez |
geographic_facet |
Antarctic Argentina Coria King George Island Potter Cove Silvia The Antarctic Vazquez |
genre |
Antarc* Antarctic Antarctica King George Island Polar Research |
genre_facet |
Antarc* Antarctic Antarctica King George Island Polar Research |
op_relation |
info:eu-repo/semantics/altIdentifier/url/http://www.degruyter.com/view/j/popore.2013.34.issue-3/popore-2013-0014/popore-2013-0014.xml http://hdl.handle.net/11336/2746 Tropeano, Mauro; Vazquez, Susana Claudia; Coria, Silvia; Turjanski, Adrian; Cicero, Daniel; et al.; Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic; De Gruyter Open; Polish Polar Research; 34; 3; 10-2013; 253-267 0138-0338 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
container_title |
Polish Polar Research |
container_volume |
34 |
container_issue |
3 |
container_start_page |
253 |
op_container_end_page |
267 |
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1779321729661796352 |