Gluconeogenic pathway does not display metabolic cold adaptation in liver of Antarctic notothenioid fish
Antarctic notothenioid fish display specializations related to cope with their chronically cold environment, such as high triacylglycerol (TAG) content in tissues. The metabolic fate of glycerol, a product of TAG mobilization, has not been studied in Antarctic fish. To assess the importance of glyce...
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ftconicet:oai:ri.conicet.gov.ar:11336/24151 2023-10-09T21:46:32+02:00 Gluconeogenic pathway does not display metabolic cold adaptation in liver of Antarctic notothenioid fish Magnoni, Leonardo Julián Scarlato, Norberto A. Ojeda, F. Patricio Wöhler, Otto C. application/pdf http://hdl.handle.net/11336/24151 eng eng Springer info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00300-013-1292-x info:eu-repo/semantics/altIdentifier/doi/10.1007/s00300-013-1292-x http://hdl.handle.net/11336/24151 Magnoni, Leonardo Julián; Scarlato, Norberto A.; Ojeda, F. Patricio; Wöhler, Otto C.; Gluconeogenic pathway does not display metabolic cold adaptation in liver of Antarctic notothenioid fish; Springer; Polar Biology; 36; 5; 5-2013; 661-671 0722-4060 CONICET Digital CONICET info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ Gluconeogenesis Glycerol Tag Mobilization Antarctic Notothenioid Fish Metabolic Cold Adaptation https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1007/s00300-013-1292-x 2023-09-24T20:16:09Z Antarctic notothenioid fish display specializations related to cope with their chronically cold environment, such as high triacylglycerol (TAG) content in tissues. The metabolic fate of glycerol, a product of TAG mobilization, has not been studied in Antarctic fish. To assess the importance of glycerol as a substrate for gluconeogenesis and to determine whether this pathway is metabolically cold adapted (MCA), key hepatic enzyme activities were measured in Antarctic (Notothenia coriiceps, Gobionotothen gibberifrons, and Chionodraco rastrospinosus) and nonAntarctic (Dissostichus eleginoides, Patagonotothen ramsayi, and Eleginops maclovinus) notothenioid fish. Fructose 1,6-biphosphatase (FBP), phosphoenolpyruvate carboxykinase (PEPCK), and glycerol kinase (GK) activities were similar in both groups at common temperatures (1, 6, 11, or 21 C). In particular, thermal sensitivity for the reactions catalyzed by FBP and PEPCK was analogous between Antarctic and non-Antarctic species, reflected by similar values for Arrhenius energy of activation (Ea) and Q10. Additionally, hepatic glycerol, glucose, and glycogen contents together with plasma glycerol and glucose concentrations were similar for all of the species studied. Our results do not support the concept of MCA in hepatic gluconeogenesis and may indicate that the use of glycerol as a precursor for glucose synthesis by this pathway is of low physiological importance in Antarctic fish. Fil: Magnoni, Leonardo Julián. University Of Maine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas Argentina Fil: Scarlato, Norberto A. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina Fil: Ojeda, F. Patricio. Pontificia Universidad Católica de Chile; Chile Fil: Wöhler, Otto C. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina. Consejo Nacional de Investigaciones ... Article in Journal/Newspaper Antarc* Antarctic Polar Biology CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Antarctic Argentina Polar Biology 36 5 661 671 |
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CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
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language |
English |
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Gluconeogenesis Glycerol Tag Mobilization Antarctic Notothenioid Fish Metabolic Cold Adaptation https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
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Gluconeogenesis Glycerol Tag Mobilization Antarctic Notothenioid Fish Metabolic Cold Adaptation https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Magnoni, Leonardo Julián Scarlato, Norberto A. Ojeda, F. Patricio Wöhler, Otto C. Gluconeogenic pathway does not display metabolic cold adaptation in liver of Antarctic notothenioid fish |
topic_facet |
Gluconeogenesis Glycerol Tag Mobilization Antarctic Notothenioid Fish Metabolic Cold Adaptation https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
description |
Antarctic notothenioid fish display specializations related to cope with their chronically cold environment, such as high triacylglycerol (TAG) content in tissues. The metabolic fate of glycerol, a product of TAG mobilization, has not been studied in Antarctic fish. To assess the importance of glycerol as a substrate for gluconeogenesis and to determine whether this pathway is metabolically cold adapted (MCA), key hepatic enzyme activities were measured in Antarctic (Notothenia coriiceps, Gobionotothen gibberifrons, and Chionodraco rastrospinosus) and nonAntarctic (Dissostichus eleginoides, Patagonotothen ramsayi, and Eleginops maclovinus) notothenioid fish. Fructose 1,6-biphosphatase (FBP), phosphoenolpyruvate carboxykinase (PEPCK), and glycerol kinase (GK) activities were similar in both groups at common temperatures (1, 6, 11, or 21 C). In particular, thermal sensitivity for the reactions catalyzed by FBP and PEPCK was analogous between Antarctic and non-Antarctic species, reflected by similar values for Arrhenius energy of activation (Ea) and Q10. Additionally, hepatic glycerol, glucose, and glycogen contents together with plasma glycerol and glucose concentrations were similar for all of the species studied. Our results do not support the concept of MCA in hepatic gluconeogenesis and may indicate that the use of glycerol as a precursor for glucose synthesis by this pathway is of low physiological importance in Antarctic fish. Fil: Magnoni, Leonardo Julián. University Of Maine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas Argentina Fil: Scarlato, Norberto A. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina Fil: Ojeda, F. Patricio. Pontificia Universidad Católica de Chile; Chile Fil: Wöhler, Otto C. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina. Consejo Nacional de Investigaciones ... |
format |
Article in Journal/Newspaper |
author |
Magnoni, Leonardo Julián Scarlato, Norberto A. Ojeda, F. Patricio Wöhler, Otto C. |
author_facet |
Magnoni, Leonardo Julián Scarlato, Norberto A. Ojeda, F. Patricio Wöhler, Otto C. |
author_sort |
Magnoni, Leonardo Julián |
title |
Gluconeogenic pathway does not display metabolic cold adaptation in liver of Antarctic notothenioid fish |
title_short |
Gluconeogenic pathway does not display metabolic cold adaptation in liver of Antarctic notothenioid fish |
title_full |
Gluconeogenic pathway does not display metabolic cold adaptation in liver of Antarctic notothenioid fish |
title_fullStr |
Gluconeogenic pathway does not display metabolic cold adaptation in liver of Antarctic notothenioid fish |
title_full_unstemmed |
Gluconeogenic pathway does not display metabolic cold adaptation in liver of Antarctic notothenioid fish |
title_sort |
gluconeogenic pathway does not display metabolic cold adaptation in liver of antarctic notothenioid fish |
publisher |
Springer |
url |
http://hdl.handle.net/11336/24151 |
geographic |
Antarctic Argentina |
geographic_facet |
Antarctic Argentina |
genre |
Antarc* Antarctic Polar Biology |
genre_facet |
Antarc* Antarctic Polar Biology |
op_relation |
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00300-013-1292-x info:eu-repo/semantics/altIdentifier/doi/10.1007/s00300-013-1292-x http://hdl.handle.net/11336/24151 Magnoni, Leonardo Julián; Scarlato, Norberto A.; Ojeda, F. Patricio; Wöhler, Otto C.; Gluconeogenic pathway does not display metabolic cold adaptation in liver of Antarctic notothenioid fish; Springer; Polar Biology; 36; 5; 5-2013; 661-671 0722-4060 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1007/s00300-013-1292-x |
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Polar Biology |
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36 |
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661 |
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671 |
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1779322256575430656 |