Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great...
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ftconicet:oai:ri.conicet.gov.ar:11336/238904 2024-09-15T17:47:07+00:00 Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce Darqui, Flavia Soledad Radonic, Laura Mabel Beracochea, Valeria Cecilia Hopp, Horacio Esteban Lopez Bilbao, Marisa application/pdf http://hdl.handle.net/11336/238904 eng eng Frontiers Media info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2021.767459 info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.767459/full http://hdl.handle.net/11336/238904 Darqui, Flavia Soledad; Radonic, Laura Mabel; Beracochea, Valeria Cecilia; Hopp, Horacio Esteban; Lopez Bilbao, Marisa; Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce; Frontiers Media; Frontiers in Plant Science; 12; 767459; 11-2021; 1-10 1664-462X CONICET Digital CONICET info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ ASTERACEAE LETTUCE MODEL SPECIES SUNFLOWER TRANSGENESIS https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.3389/fpls.2021.767459 2024-07-08T23:38:08Z The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great biodiversity, covering all environments on the planet, except Antarctica. Also, it includes economically important crops, such as lettuce, sunflower, and chrysanthemum; wild flowers; herbs, and several species that produce molecules with pharmacological properties. Nevertheless, the biotechnological improvement of this family is limited to a few species and their genetic transformation was achieved later than in other plant families. Lettuce (Lactuca sativa L.) is a model species in molecular biology and plant biotechnology that has easily adapted to tissue culture, with efficient shoot regeneration from different tissues, organs, cells, and protoplasts. Due to this plasticity, it was possible to obtain transgenic plants tolerant to biotic or abiotic stresses as well as for the production of commercially interesting molecules (molecular farming). These advances, together with the complete sequencing of lettuce genome allowed the rapid adoption of gene editing using the CRISPR system. On the other hand, sunflower (Helianthus annuus L.) is a species that for years was considered recalcitrant to in vitro culture. Although this difficulty was overcome and some publications were made on sunflower genetic transformation, until now there is no transgenic variety commercialized or authorized for cultivation. In this article, we review similarities (such as avoiding the utilization of the CaMV35S promoter in transformation vectors) and differences (such as transformation efficiency) in the state of the art of genetic transformation techniques performed in these two species. Fil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de ... Article in Journal/Newspaper Antarc* Antarctica CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Frontiers in Plant Science 12 |
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CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
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English |
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ASTERACEAE LETTUCE MODEL SPECIES SUNFLOWER TRANSGENESIS https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
spellingShingle |
ASTERACEAE LETTUCE MODEL SPECIES SUNFLOWER TRANSGENESIS https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 Darqui, Flavia Soledad Radonic, Laura Mabel Beracochea, Valeria Cecilia Hopp, Horacio Esteban Lopez Bilbao, Marisa Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce |
topic_facet |
ASTERACEAE LETTUCE MODEL SPECIES SUNFLOWER TRANSGENESIS https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
description |
The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great biodiversity, covering all environments on the planet, except Antarctica. Also, it includes economically important crops, such as lettuce, sunflower, and chrysanthemum; wild flowers; herbs, and several species that produce molecules with pharmacological properties. Nevertheless, the biotechnological improvement of this family is limited to a few species and their genetic transformation was achieved later than in other plant families. Lettuce (Lactuca sativa L.) is a model species in molecular biology and plant biotechnology that has easily adapted to tissue culture, with efficient shoot regeneration from different tissues, organs, cells, and protoplasts. Due to this plasticity, it was possible to obtain transgenic plants tolerant to biotic or abiotic stresses as well as for the production of commercially interesting molecules (molecular farming). These advances, together with the complete sequencing of lettuce genome allowed the rapid adoption of gene editing using the CRISPR system. On the other hand, sunflower (Helianthus annuus L.) is a species that for years was considered recalcitrant to in vitro culture. Although this difficulty was overcome and some publications were made on sunflower genetic transformation, until now there is no transgenic variety commercialized or authorized for cultivation. In this article, we review similarities (such as avoiding the utilization of the CaMV35S promoter in transformation vectors) and differences (such as transformation efficiency) in the state of the art of genetic transformation techniques performed in these two species. Fil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de ... |
format |
Article in Journal/Newspaper |
author |
Darqui, Flavia Soledad Radonic, Laura Mabel Beracochea, Valeria Cecilia Hopp, Horacio Esteban Lopez Bilbao, Marisa |
author_facet |
Darqui, Flavia Soledad Radonic, Laura Mabel Beracochea, Valeria Cecilia Hopp, Horacio Esteban Lopez Bilbao, Marisa |
author_sort |
Darqui, Flavia Soledad |
title |
Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce |
title_short |
Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce |
title_full |
Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce |
title_fullStr |
Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce |
title_full_unstemmed |
Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce |
title_sort |
peculiarities of the transformation of asteraceae family species: the cases of sunflower and lettuce |
publisher |
Frontiers Media |
url |
http://hdl.handle.net/11336/238904 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2021.767459 info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.767459/full http://hdl.handle.net/11336/238904 Darqui, Flavia Soledad; Radonic, Laura Mabel; Beracochea, Valeria Cecilia; Hopp, Horacio Esteban; Lopez Bilbao, Marisa; Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce; Frontiers Media; Frontiers in Plant Science; 12; 767459; 11-2021; 1-10 1664-462X CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
op_doi |
https://doi.org/10.3389/fpls.2021.767459 |
container_title |
Frontiers in Plant Science |
container_volume |
12 |
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1810495748345692160 |