Pectin extraction from lime pomace by cold-active polygalacturonase-assisted method

• Published in: International Journal of Biological Macromolecules
• Main Authors: Bezus, Brenda, Contreras Esquivel, Juan Carlos, Cavalitto, Sebastian Fernando, Cavello, Ivana Alejandra
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier Science
• Subjects: ANTARCTIC YEAST; CITRUS AURANTIFOLIA; COLD-ACTIVE ENZYME; TAUSONIA PULLULANS; https://purl.org/becyt/ford/2.9; https://purl.org/becyt/ford/2; Antarctic; Argentina; Contreras; Esquivel; Antarc*
• Online Access: http://hdl.handle.net/11336/211173

An Antarctic yeast was cultivated to produce and enzymatic extract with polygalacturonase activity, whose biochemical properties were studied. It assisted pectin extraction from lime pomace at 20 °C. The extract produced by Tausonia pullulans 8E had an optimum temperature of 40 °C and optimum pH of 5.0. At 20 °C, it displayed 54% of relative activity. A good pH-stability and thermostability were observed. Hg+2 and Co+2 inhibited its activity in 40 and 11%, respectively. Pectin was obtained from lime pomace at 20 °C, with 15% of yield after 120 min extraction. Uronic acid (46%) and neutral sugars (53%) were determined. Pectin's molecular weight was estimated in the order of 100,000 g mol−1. By anion-exchange chromatography, pectin-linked and free neutral sugars were observed. The high-methoxyl pectin was used to prepare low-calorie gels. It was demonstrated that this cold-active enzyme allows enzymatic-assisted pectin extraction at 20 °C. Fil: Bezus, Brenda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Contreras Esquivel, Juan Carlos. Universidad Autonoma de Coahuila; México Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y ...