Pectin extraction from lime pomace by cold-active polygalacturonase-assisted method
An Antarctic yeast was cultivated to produce and enzymatic extract with polygalacturonase activity, whose biochemical properties were studied. It assisted pectin extraction from lime pomace at 20 °C. The extract produced by Tausonia pullulans 8E had an optimum temperature of 40 °C and optimum pH of...
Published in: | International Journal of Biological Macromolecules |
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Language: | English |
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ftconicet:oai:ri.conicet.gov.ar:11336/211173 2023-10-09T21:47:16+02:00 Pectin extraction from lime pomace by cold-active polygalacturonase-assisted method Bezus, Brenda Contreras Esquivel, Juan Carlos Cavalitto, Sebastian Fernando Cavello, Ivana Alejandra application/pdf http://hdl.handle.net/11336/211173 eng eng Elsevier Science info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0141813022007188 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2022.04.019 http://hdl.handle.net/11336/211173 Bezus, Brenda; Contreras Esquivel, Juan Carlos; Cavalitto, Sebastian Fernando; Cavello, Ivana Alejandra; Pectin extraction from lime pomace by cold-active polygalacturonase-assisted method; Elsevier Science; International Journal of Biological Macromolecules; 209; 6-2022; 290-298 0141-8130 CONICET Digital CONICET info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ ANTARCTIC YEAST CITRUS AURANTIFOLIA COLD-ACTIVE ENZYME TAUSONIA PULLULANS https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1016/j.ijbiomac.2022.04.019 2023-09-24T19:27:48Z An Antarctic yeast was cultivated to produce and enzymatic extract with polygalacturonase activity, whose biochemical properties were studied. It assisted pectin extraction from lime pomace at 20 °C. The extract produced by Tausonia pullulans 8E had an optimum temperature of 40 °C and optimum pH of 5.0. At 20 °C, it displayed 54% of relative activity. A good pH-stability and thermostability were observed. Hg+2 and Co+2 inhibited its activity in 40 and 11%, respectively. Pectin was obtained from lime pomace at 20 °C, with 15% of yield after 120 min extraction. Uronic acid (46%) and neutral sugars (53%) were determined. Pectin's molecular weight was estimated in the order of 100,000 g mol−1. By anion-exchange chromatography, pectin-linked and free neutral sugars were observed. The high-methoxyl pectin was used to prepare low-calorie gels. It was demonstrated that this cold-active enzyme allows enzymatic-assisted pectin extraction at 20 °C. Fil: Bezus, Brenda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Contreras Esquivel, Juan Carlos. Universidad Autonoma de Coahuila; México Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y ... Article in Journal/Newspaper Antarc* Antarctic CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Antarctic Argentina Contreras ENVELOPE(-63.050,-63.050,-64.883,-64.883) Esquivel ENVELOPE(-63.667,-63.667,-64.317,-64.317) International Journal of Biological Macromolecules 209 290 298 |
institution |
Open Polar |
collection |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
op_collection_id |
ftconicet |
language |
English |
topic |
ANTARCTIC YEAST CITRUS AURANTIFOLIA COLD-ACTIVE ENZYME TAUSONIA PULLULANS https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
spellingShingle |
ANTARCTIC YEAST CITRUS AURANTIFOLIA COLD-ACTIVE ENZYME TAUSONIA PULLULANS https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 Bezus, Brenda Contreras Esquivel, Juan Carlos Cavalitto, Sebastian Fernando Cavello, Ivana Alejandra Pectin extraction from lime pomace by cold-active polygalacturonase-assisted method |
topic_facet |
ANTARCTIC YEAST CITRUS AURANTIFOLIA COLD-ACTIVE ENZYME TAUSONIA PULLULANS https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
description |
An Antarctic yeast was cultivated to produce and enzymatic extract with polygalacturonase activity, whose biochemical properties were studied. It assisted pectin extraction from lime pomace at 20 °C. The extract produced by Tausonia pullulans 8E had an optimum temperature of 40 °C and optimum pH of 5.0. At 20 °C, it displayed 54% of relative activity. A good pH-stability and thermostability were observed. Hg+2 and Co+2 inhibited its activity in 40 and 11%, respectively. Pectin was obtained from lime pomace at 20 °C, with 15% of yield after 120 min extraction. Uronic acid (46%) and neutral sugars (53%) were determined. Pectin's molecular weight was estimated in the order of 100,000 g mol−1. By anion-exchange chromatography, pectin-linked and free neutral sugars were observed. The high-methoxyl pectin was used to prepare low-calorie gels. It was demonstrated that this cold-active enzyme allows enzymatic-assisted pectin extraction at 20 °C. Fil: Bezus, Brenda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Contreras Esquivel, Juan Carlos. Universidad Autonoma de Coahuila; México Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y ... |
format |
Article in Journal/Newspaper |
author |
Bezus, Brenda Contreras Esquivel, Juan Carlos Cavalitto, Sebastian Fernando Cavello, Ivana Alejandra |
author_facet |
Bezus, Brenda Contreras Esquivel, Juan Carlos Cavalitto, Sebastian Fernando Cavello, Ivana Alejandra |
author_sort |
Bezus, Brenda |
title |
Pectin extraction from lime pomace by cold-active polygalacturonase-assisted method |
title_short |
Pectin extraction from lime pomace by cold-active polygalacturonase-assisted method |
title_full |
Pectin extraction from lime pomace by cold-active polygalacturonase-assisted method |
title_fullStr |
Pectin extraction from lime pomace by cold-active polygalacturonase-assisted method |
title_full_unstemmed |
Pectin extraction from lime pomace by cold-active polygalacturonase-assisted method |
title_sort |
pectin extraction from lime pomace by cold-active polygalacturonase-assisted method |
publisher |
Elsevier Science |
url |
http://hdl.handle.net/11336/211173 |
long_lat |
ENVELOPE(-63.050,-63.050,-64.883,-64.883) ENVELOPE(-63.667,-63.667,-64.317,-64.317) |
geographic |
Antarctic Argentina Contreras Esquivel |
geographic_facet |
Antarctic Argentina Contreras Esquivel |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0141813022007188 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2022.04.019 http://hdl.handle.net/11336/211173 Bezus, Brenda; Contreras Esquivel, Juan Carlos; Cavalitto, Sebastian Fernando; Cavello, Ivana Alejandra; Pectin extraction from lime pomace by cold-active polygalacturonase-assisted method; Elsevier Science; International Journal of Biological Macromolecules; 209; 6-2022; 290-298 0141-8130 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1016/j.ijbiomac.2022.04.019 |
container_title |
International Journal of Biological Macromolecules |
container_volume |
209 |
container_start_page |
290 |
op_container_end_page |
298 |
_version_ |
1779310262383280128 |