Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide
Enzymatic synthesis of fatty acid ethyl esters in supercritical and near-critical carbon dioxide using lipase B from Candida antarctica (CALB) immobilized onto chitosan is here investigated. The objective was to evaluate the operational parameters that influence the enzymatic esterification reaction...
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ftconicet:oai:ri.conicet.gov.ar:11336/20798 2023-10-09T21:46:58+02:00 Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide dos Santos, Jose C. S. Bonazza, Horacio de Matos, Leonardo J. B. L. Carneiro, Elizabete A. Barbosa, Oveimar Fernandez Lafuente, Roberto Gonçalves, Luciana R.B. de Sant' Ana, Hosiberto B. Aguiar, Rílvia S. Santiago application/pdf http://hdl.handle.net/11336/20798 eng eng Elsevier info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2215017X16300911 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.btre.2017.02.003 http://hdl.handle.net/11336/20798 dos Santos, Jose C. S.; Bonazza, Horacio; de Matos, Leonardo J. B. L.; Carneiro, Elizabete A.; Barbosa, Oveimar; et al.; Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide; Elsevier; Biotechnology Reports; 14; 3-2017; 16-26 2215-017X CONICET Digital CONICET info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ CalB Lipase chitosan synthesis ethyl oleate https://purl.org/becyt/ford/2.4 https://purl.org/becyt/ford/2 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1016/j.btre.2017.02.003 2023-09-24T18:40:51Z Enzymatic synthesis of fatty acid ethyl esters in supercritical and near-critical carbon dioxide using lipase B from Candida antarctica (CALB) immobilized onto chitosan is here investigated. The objective was to evaluate the operational parameters that influence the enzymatic esterification reaction between oleic acid and ethanol, conducted in supercritical carbon dioxide, as a route to produce ethyl oleate. Biocatalyst has been prepared through the immobilization of CALB by covalent bond using chitosan activated with glycidol, ethylenediamine (EDA) and glutaraldehyde as support. In order to determine the best operational conditions of the esterification reaction, an experimental design (23) was conducted to evaluate the effect of the following parameters: alcohol to oil molar ratios, reaction time and temperature. The maximum conversion was 46.9% at a temperature of 29.9 °C, ethanol to oleic acid molar ratio equal to 4.50:1, and a reaction time of 6.5 hours. Additionally, the removal of water from the medium, by using zeolite, promoted an increase in the conversion of oleic acid into ethyl esters. These results show that the combined use of supercritical carbon dioxide and zeolite favors the enzymatic esterification reaction, increasing the yields. Fil: dos Santos, Jose C. S. Universidade da Integração Internacional da Lusofonia Afro-Brasileira; Brasil Fil: Bonazza, Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina Fil: de Matos, Leonardo J. B. L. Universidade Federal do Ceará; Brasil Fil: Carneiro, Elizabete A. Universidade Federal do Ceará; Brasil Fil: Barbosa, Oveimar. Universidad del Tolima; Colombia Fil: Fernandez Lafuente, Roberto. Universidad Autónoma de Madrid; España Fil: Gonçalves, Luciana R.B. Universidade Federal do Ceará; Brasil Fil: de Sant' Ana, ... Article in Journal/Newspaper Antarc* Antarctica CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Argentina Fernandez ENVELOPE(-62.233,-62.233,-63.250,-63.250) Biotechnology Reports 14 16 26 |
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CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
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ftconicet |
language |
English |
topic |
CalB Lipase chitosan synthesis ethyl oleate https://purl.org/becyt/ford/2.4 https://purl.org/becyt/ford/2 |
spellingShingle |
CalB Lipase chitosan synthesis ethyl oleate https://purl.org/becyt/ford/2.4 https://purl.org/becyt/ford/2 dos Santos, Jose C. S. Bonazza, Horacio de Matos, Leonardo J. B. L. Carneiro, Elizabete A. Barbosa, Oveimar Fernandez Lafuente, Roberto Gonçalves, Luciana R.B. de Sant' Ana, Hosiberto B. Aguiar, Rílvia S. Santiago Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide |
topic_facet |
CalB Lipase chitosan synthesis ethyl oleate https://purl.org/becyt/ford/2.4 https://purl.org/becyt/ford/2 |
description |
Enzymatic synthesis of fatty acid ethyl esters in supercritical and near-critical carbon dioxide using lipase B from Candida antarctica (CALB) immobilized onto chitosan is here investigated. The objective was to evaluate the operational parameters that influence the enzymatic esterification reaction between oleic acid and ethanol, conducted in supercritical carbon dioxide, as a route to produce ethyl oleate. Biocatalyst has been prepared through the immobilization of CALB by covalent bond using chitosan activated with glycidol, ethylenediamine (EDA) and glutaraldehyde as support. In order to determine the best operational conditions of the esterification reaction, an experimental design (23) was conducted to evaluate the effect of the following parameters: alcohol to oil molar ratios, reaction time and temperature. The maximum conversion was 46.9% at a temperature of 29.9 °C, ethanol to oleic acid molar ratio equal to 4.50:1, and a reaction time of 6.5 hours. Additionally, the removal of water from the medium, by using zeolite, promoted an increase in the conversion of oleic acid into ethyl esters. These results show that the combined use of supercritical carbon dioxide and zeolite favors the enzymatic esterification reaction, increasing the yields. Fil: dos Santos, Jose C. S. Universidade da Integração Internacional da Lusofonia Afro-Brasileira; Brasil Fil: Bonazza, Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina Fil: de Matos, Leonardo J. B. L. Universidade Federal do Ceará; Brasil Fil: Carneiro, Elizabete A. Universidade Federal do Ceará; Brasil Fil: Barbosa, Oveimar. Universidad del Tolima; Colombia Fil: Fernandez Lafuente, Roberto. Universidad Autónoma de Madrid; España Fil: Gonçalves, Luciana R.B. Universidade Federal do Ceará; Brasil Fil: de Sant' Ana, ... |
format |
Article in Journal/Newspaper |
author |
dos Santos, Jose C. S. Bonazza, Horacio de Matos, Leonardo J. B. L. Carneiro, Elizabete A. Barbosa, Oveimar Fernandez Lafuente, Roberto Gonçalves, Luciana R.B. de Sant' Ana, Hosiberto B. Aguiar, Rílvia S. Santiago |
author_facet |
dos Santos, Jose C. S. Bonazza, Horacio de Matos, Leonardo J. B. L. Carneiro, Elizabete A. Barbosa, Oveimar Fernandez Lafuente, Roberto Gonçalves, Luciana R.B. de Sant' Ana, Hosiberto B. Aguiar, Rílvia S. Santiago |
author_sort |
dos Santos, Jose C. S. |
title |
Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide |
title_short |
Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide |
title_full |
Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide |
title_fullStr |
Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide |
title_full_unstemmed |
Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide |
title_sort |
immobilization of calb on activated chitosan: application to enzymatic synthesis in supercritical and near-critical carbon dioxide |
publisher |
Elsevier |
url |
http://hdl.handle.net/11336/20798 |
long_lat |
ENVELOPE(-62.233,-62.233,-63.250,-63.250) |
geographic |
Argentina Fernandez |
geographic_facet |
Argentina Fernandez |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2215017X16300911 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.btre.2017.02.003 http://hdl.handle.net/11336/20798 dos Santos, Jose C. S.; Bonazza, Horacio; de Matos, Leonardo J. B. L.; Carneiro, Elizabete A.; Barbosa, Oveimar; et al.; Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide; Elsevier; Biotechnology Reports; 14; 3-2017; 16-26 2215-017X CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1016/j.btre.2017.02.003 |
container_title |
Biotechnology Reports |
container_volume |
14 |
container_start_page |
16 |
op_container_end_page |
26 |
_version_ |
1779309611988287488 |