Prokaryotic diversity and biogeochemical characteristics of benthic microbial ecosystems from James Ross Archipelago (West Antarctica)

• Published in: Polar Biology
• Main Authors: Fernández, Guillermo César, Lecomte, Karina Leticia, Vignoni, Paula, Soto Rueda, Eliana Marcela, Coria, Silvia Herminda, Lirio, Juan Manuel, Mlewski, Estela Cecilia
• Format: Article in Journal/Newspaper
• Language: English
• Published: Springer
• Subjects: 16S RRNA GENES; ANTARCTICA; JAMES ROSS ARCHIPELAGO; MICROBIAL DIVERSITY; MICROBIAL MATS; https://purl.org/becyt/ford/1.5; https://purl.org/becyt/ford/1; Antarctic; Roja; Ross Archipelago; Ross Island; Vega Island; West Antarctica; Antarc*; Antarctica; James Ross Island; Polar Biology
• Online Access: http://hdl.handle.net/11336/193950

The James Ross archipelago houses numerous lakes and ponds. In this region, a vast diatom and cyanobacterial variety has been reported; however, the prokaryotic diversity in microbial mats from these lakes remains poorly explored. Here, a high-throughput sequencing of 16S rRNA gene in microbial mats from Lake Bart-Roja in James Ross Island and lakes Pan Negro and North Pan Negro located in Vega Island was performed. Combined with mineralogical and environmental characteristics, we analyzed the diversity and structure of the microbial communities. Sequences assigned to Archaea were extremely low, while Bacteria domain prevailed with the abundance of Proteobacteria (mostly Betaproteobacteriales) followed by Bacteroidetes, Verrucomicrobia, Firmicutes, and Cyanobacteria. Local environmental conditions, such as conductivity and Eh, provided differential microbial assemblages that might have implications in the oligotrophic status of the lakes. Consequently, a clear segregation at the family level was observed. In this sense, the assigned diversity was related to taxa recognized as denitrifiers and sulfur oxidizers. Particularly, in Lake Pan Negro sulfur-reducing and methanogenic representatives were also found and positively correlate with alkalinity and water depth. Moreover, Deinococcus-Thermus was observed in Lake Bart-Roja, while Melainabacteria (Cyanobacteria)—poorly reported in Antarctic mats—was detected in Lake Pan Negro. Epsilonbacteraeota was exclusively found in this lake, suggesting new potential phylotypes. This study contributes to the understanding of the diversity, composition, and structure of Antarctic benthic microbial ecosystems and provides highly valuable information, which can be used as a proxy to evaluate environmental changes affecting Antarctic microbiota. Fil: Fernández, Guillermo César. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad ...