Revalorization of chicken feather waste into a high antioxidant activity feather protein hydrolysate using a novel psychrotolerant bacterium

• Published in: Biocatalysis and Agricultural Biotechnology
• Main Authors: Bezus, Brenda, Ruscasso, Maria Florencia, Garmendia, Gabriela, Vero, Silvana, Cavello, Ivana Alejandra, Cavalitto, Sebastian Fernando
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier
• Subjects: ANTARCTIC BACTERIUM; ANTIOXIDANT ACTIVITY; FEATHER PROTEIN HYDROLYSATE; https://purl.org/becyt/ford/2.9; https://purl.org/becyt/ford/2; Antarctic; Argentina; Uruguay; Antarc*
• Online Access: http://hdl.handle.net/11336/181100

In this study, we assessed the capacity of Pedobacter sp. 3.14.7 —a novel Antarctic keratinolytic bacterium— of producing a feather protein hydrolysate (FPH) with high antioxidant activity. This strain was able to degrade raw feather into protein hydrolysate under optimal conditions of 30 g l−1 of feather concentration, pH 6.0, and 20 °C. Maximum enzymatic activity was detected after a 192-h cultivation period (125.67 ± 3.06 U ml−1). Quenching of the ABTS radical (2,20-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid) by the hydrolysate reached a value of approximately 95%, with a 50% Inhibitory Concentration (IC50) value of 0.57 ± 0.01 g l−1. An interesting reducing power of the FPH towards Fe+3 was also detected (2.19 Abs700 units in the presence of 2.03 g l−1 protein). The hydrolysate also displayed a moderate nitric oxide-scavenging activity, with an IC50 of 0.53 ± 0.01 g l−1. This FPH's free amino acid content is high (almost 5 g l−1). Results indicate that this hydrolysate may be useful as supplementary protein and antioxidant in animal feed formulations. Fil: Bezus, Brenda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Ruscasso, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Garmendia, Gabriela. Universidad de la República; Uruguay Fil: Vero, Silvana. Universidad de la República; Uruguay Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. ...