Response to lethal UVA radiation in the Antarctic bacterium Pseudomonas extremaustralis: polyhydroxybutyrate and cold adaptation as protective factors

• Published in: Extremophiles
• Main Authors: Tribelli, Paula Maria, Pezzoni, Magdalena, Brito, María Gabriela, Montesinos, Victor Nahuel, Costa, Cristina Susana, López, Nancy Beatriz
• Format: Article in Journal/Newspaper
• Language: English
• Published: Springer Tokyo
• Subjects: ANTARCTICA; COLD ADAPTATION; PHB; POLYHYDROHYDROXYALKANOATES; PSEUDOMONAS EXTREMAUSTRALIS; ULTRAVIOLET RADIATION; https://purl.org/becyt/ford/1.6; https://purl.org/becyt/ford/1; Antarctic; The Antarctic; Argentina; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/11336/175236

Pseudomonas extremaustralis is an Antarctic bacterium with high stress resistance, able to grow under cold conditions. It is capable to produce polyhydroxyalkanoates (PHAs) mainly as polyhydroxybutyrate (PHB) and, to a lesser extent, medium-chain length polyhydroxyalkanoates (mclPHAs). In this work, we analyzed the role of PHAs and cold adaptation in the survival of P. extremaustralis after lethal UVA exposure. P. extremaustralis presented higher radiation resistance under polymer accumulation conditions. This result was also observed in the derivative mutant strain PHA−, deficient for mclPHAs production. On the contrary, the PHB− derivative mutant, deficient for PHB production, showed high sensitivity to UVA exposure. Complementation of the PHB− strain restored the wild-type resistance level, indicating that the UVA-sensitive phenotype is due to the lack of PHB. All strains exhibited high sensitivity to radiation when cultured under PHAs non-accumulation conditions. A slight decrease in PHB content was observed after UVA exposure in association with increased survival. The scattering of UVA radiation by intracellular PHAs granules could also result in bacterial cell protection. In addition, cold conditions improved UVA tolerance, probably depending on PHB mobilization. Results showed that PHB accumulation is crucial in the resistance to UVA in P. extremaustralis. Mechanisms involved probably entail depolymerization and light scattering acting as a screen, both conferring protection against oxidative stress. Fil: Tribelli, Paula Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Pezzoni, Magdalena. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. ...