Molecular mechanisms underlying responses of the Antarctic coral Malacobelemnon daytoni to ocean acidification
Benthic organisms of the Southern Ocean are particularly vulnerable to ocean acidification (OA), as they inhabit cold waters where calcite-aragonite saturation states are naturally low. OA most strongly affects animals with calcium carbonate skeletons or shells, such as corals and mollusks. We expos...
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ftconicet:oai:ri.conicet.gov.ar:11336/170647 2023-10-09T21:47:00+02:00 Molecular mechanisms underlying responses of the Antarctic coral Malacobelemnon daytoni to ocean acidification Servetto, Natalia de Aranzamendi, Maria Carla Bettencourt, R. Held, Christoph Abele, D. Movilla, J. Gonzalez, Germán Alexis Bustos, Diego Martin Sahade, Ricardo Jose application/pdf http://hdl.handle.net/11336/170647 eng eng Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.marenvres.2021.105430 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0141113621001860 http://hdl.handle.net/11336/170647 Servetto, Natalia; de Aranzamendi, Maria Carla; Bettencourt, R.; Held, Christoph; Abele, D.; et al.; Molecular mechanisms underlying responses of the Antarctic coral Malacobelemnon daytoni to ocean acidification; Elsevier; Marine Environmental Research; 170; 8-2021; 1-13 0141-1136 CONICET Digital CONICET info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ ANTARCTICA CORAL MALACOBELEMNON DAYTONI GENE EXPRESSION OCEAN ACIDIFICATION https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1016/j.marenvres.2021.105430 2023-09-24T18:44:01Z Benthic organisms of the Southern Ocean are particularly vulnerable to ocean acidification (OA), as they inhabit cold waters where calcite-aragonite saturation states are naturally low. OA most strongly affects animals with calcium carbonate skeletons or shells, such as corals and mollusks. We exposed the abundant cold-water coral Malacobelemnon daytoni from an Antarctic fjord to low pH seawater (LpH) (7.68 ± 0.17) to test its physiological responses to OA, at the level of gene expression (RT-PCR) and enzyme activity. Corals were exposed in short- (3 days) and long-term (54 days) experiments to two pCO2 conditions (ambient and elevated pCO2 equaling RCP 8.5, IPCC 2019, approximately 372.53 and 956.78 μatm, respectively). Of the eleven genes studied through RT-PCR, six were significantly upregulated compared with control in the short-term in the LpH condition, including the antioxidant enzyme superoxide dismutase (SOD), Heat Shock Protein 70 (HSP70), Toll-like receptor (TLR), galaxin and ferritin. After long-term exposure to low pH conditions, RT-PCR analysis showed seven genes were upregulated. These include the mannose-binding C-Lectin and HSP90. Also, the expression of TLR and galaxin, among others, continued to be upregulated after long-term exposure to LpH. Expression of carbonic anhydrase (CA), a key enzyme involved in calcification, was also significantly upregulated after long-term exposure. Our results indicated that, after two months, M. daytoni is not acclimatized to this experimental LpH condition. Gene expression profiles revealed molecular impacts that were not evident at the enzyme activity level. Consequently, understanding the molecular mechanisms behind the physiological processes in the response of a coral to LpH is critical to understanding the ability of polar species to cope with future environmental changes. Approaches integrating molecular tools into Antarctic ecological and/or conservation research make an essential contribution given the current ongoing OA processes. Fil: Servetto, ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Ocean acidification Southern Ocean CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Antarctic Southern Ocean The Antarctic Marine Environmental Research 170 105430 |
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Open Polar |
collection |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
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ftconicet |
language |
English |
topic |
ANTARCTICA CORAL MALACOBELEMNON DAYTONI GENE EXPRESSION OCEAN ACIDIFICATION https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
spellingShingle |
ANTARCTICA CORAL MALACOBELEMNON DAYTONI GENE EXPRESSION OCEAN ACIDIFICATION https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Servetto, Natalia de Aranzamendi, Maria Carla Bettencourt, R. Held, Christoph Abele, D. Movilla, J. Gonzalez, Germán Alexis Bustos, Diego Martin Sahade, Ricardo Jose Molecular mechanisms underlying responses of the Antarctic coral Malacobelemnon daytoni to ocean acidification |
topic_facet |
ANTARCTICA CORAL MALACOBELEMNON DAYTONI GENE EXPRESSION OCEAN ACIDIFICATION https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
description |
Benthic organisms of the Southern Ocean are particularly vulnerable to ocean acidification (OA), as they inhabit cold waters where calcite-aragonite saturation states are naturally low. OA most strongly affects animals with calcium carbonate skeletons or shells, such as corals and mollusks. We exposed the abundant cold-water coral Malacobelemnon daytoni from an Antarctic fjord to low pH seawater (LpH) (7.68 ± 0.17) to test its physiological responses to OA, at the level of gene expression (RT-PCR) and enzyme activity. Corals were exposed in short- (3 days) and long-term (54 days) experiments to two pCO2 conditions (ambient and elevated pCO2 equaling RCP 8.5, IPCC 2019, approximately 372.53 and 956.78 μatm, respectively). Of the eleven genes studied through RT-PCR, six were significantly upregulated compared with control in the short-term in the LpH condition, including the antioxidant enzyme superoxide dismutase (SOD), Heat Shock Protein 70 (HSP70), Toll-like receptor (TLR), galaxin and ferritin. After long-term exposure to low pH conditions, RT-PCR analysis showed seven genes were upregulated. These include the mannose-binding C-Lectin and HSP90. Also, the expression of TLR and galaxin, among others, continued to be upregulated after long-term exposure to LpH. Expression of carbonic anhydrase (CA), a key enzyme involved in calcification, was also significantly upregulated after long-term exposure. Our results indicated that, after two months, M. daytoni is not acclimatized to this experimental LpH condition. Gene expression profiles revealed molecular impacts that were not evident at the enzyme activity level. Consequently, understanding the molecular mechanisms behind the physiological processes in the response of a coral to LpH is critical to understanding the ability of polar species to cope with future environmental changes. Approaches integrating molecular tools into Antarctic ecological and/or conservation research make an essential contribution given the current ongoing OA processes. Fil: Servetto, ... |
format |
Article in Journal/Newspaper |
author |
Servetto, Natalia de Aranzamendi, Maria Carla Bettencourt, R. Held, Christoph Abele, D. Movilla, J. Gonzalez, Germán Alexis Bustos, Diego Martin Sahade, Ricardo Jose |
author_facet |
Servetto, Natalia de Aranzamendi, Maria Carla Bettencourt, R. Held, Christoph Abele, D. Movilla, J. Gonzalez, Germán Alexis Bustos, Diego Martin Sahade, Ricardo Jose |
author_sort |
Servetto, Natalia |
title |
Molecular mechanisms underlying responses of the Antarctic coral Malacobelemnon daytoni to ocean acidification |
title_short |
Molecular mechanisms underlying responses of the Antarctic coral Malacobelemnon daytoni to ocean acidification |
title_full |
Molecular mechanisms underlying responses of the Antarctic coral Malacobelemnon daytoni to ocean acidification |
title_fullStr |
Molecular mechanisms underlying responses of the Antarctic coral Malacobelemnon daytoni to ocean acidification |
title_full_unstemmed |
Molecular mechanisms underlying responses of the Antarctic coral Malacobelemnon daytoni to ocean acidification |
title_sort |
molecular mechanisms underlying responses of the antarctic coral malacobelemnon daytoni to ocean acidification |
publisher |
Elsevier |
url |
http://hdl.handle.net/11336/170647 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Antarctica Ocean acidification Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Ocean acidification Southern Ocean |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.marenvres.2021.105430 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0141113621001860 http://hdl.handle.net/11336/170647 Servetto, Natalia; de Aranzamendi, Maria Carla; Bettencourt, R.; Held, Christoph; Abele, D.; et al.; Molecular mechanisms underlying responses of the Antarctic coral Malacobelemnon daytoni to ocean acidification; Elsevier; Marine Environmental Research; 170; 8-2021; 1-13 0141-1136 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1016/j.marenvres.2021.105430 |
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Marine Environmental Research |
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170 |
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105430 |
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1779309669388386304 |