Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla
The early ontogenetic stages of the sub-Antarctic king crab Lithodes santolla were analyzed for the presence and activities of a set of important digestive enzymes. The eggs and non-feeding larvae (zoea I-III, megalopa) showed high activities of esterases, phosphatases, and exopeptidases indicating...
Published in: | Marine Biology |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Springer
|
Subjects: | |
Online Access: | http://hdl.handle.net/11336/150639 |
id |
ftconicet:oai:ri.conicet.gov.ar:11336/150639 |
---|---|
record_format |
openpolar |
spelling |
ftconicet:oai:ri.conicet.gov.ar:11336/150639 2023-10-09T21:45:35+02:00 Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla Saborowski, R. Thatje, S. Calcagno, Javier Ángel Lovrich, Gustavo Alejandro Anger, K. application/pdf http://hdl.handle.net/11336/150639 eng eng Springer info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00227-005-0240-x info:eu-repo/semantics/altIdentifier/doi/10.1007/s00227-005-0240-x http://hdl.handle.net/11336/150639 Saborowski, R.; Thatje, S.; Calcagno, Javier Ángel; Lovrich, Gustavo Alejandro; Anger, K.; Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla; Springer; Marine Biology; 149; 4; 7-2006; 865-873 0025-3162 1432-1793 CONICET Digital CONICET info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ Enzymes Lithodes Santolla https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1007/s00227-005-0240-x 2023-09-24T18:56:27Z The early ontogenetic stages of the sub-Antarctic king crab Lithodes santolla were analyzed for the presence and activities of a set of important digestive enzymes. The eggs and non-feeding larvae (zoea I-III, megalopa) showed high activities of esterases, phosphatases, and exopeptidases indicating the enzymatic ability to utilize endogeneous yolk reserves. SDS-PAGE showed a continuous decrease of proteins or proteids in the range of 59-81 kDa during ontogenetic development from the eggs through the zoeal stages to the first juvenile crab stage, CI. This reduction reflects the degradation of storage compounds during lecithotrophic larval development. Activities of the endopeptidases, trypsin and chymotrypsin, were low in eggs and larvae but increased significantly in the first juvenile crab stage. These enzymes typically facilitate the first steps of proteolysis in the extra-cellular spaces of the midgut gland and in the stomach. Their scarcity indicates that the larvae of L. santolla are physiologically not prepared to digest external food. This ability seems to appear first in the CI stage. Extracts of juvenile midgut glands and the gastric fluids of adults showed high activities of a variety of digestive enzymes including phosphatases, carbohydrases, as well as endo- and exopeptidases. High activities of digestive enzymes in adults may compensate for scarce food supply and rate-limiting low temperatures in the predominantly sub-Antarctic habitats of L. santolla. Fil: Saborowski, R. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; Alemania Fil: Thatje, S. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; Alemania. University of Southampton; Reino Unido Fil: Calcagno, Javier Ángel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lovrich, Gustavo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones ... Article in Journal/Newspaper Antarc* Antarctic CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Antarctic Argentina Austral Gustavo ENVELOPE(-62.917,-62.917,-64.833,-64.833) Marine Biology 149 4 865 873 |
institution |
Open Polar |
collection |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
op_collection_id |
ftconicet |
language |
English |
topic |
Enzymes Lithodes Santolla https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
spellingShingle |
Enzymes Lithodes Santolla https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Saborowski, R. Thatje, S. Calcagno, Javier Ángel Lovrich, Gustavo Alejandro Anger, K. Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla |
topic_facet |
Enzymes Lithodes Santolla https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
description |
The early ontogenetic stages of the sub-Antarctic king crab Lithodes santolla were analyzed for the presence and activities of a set of important digestive enzymes. The eggs and non-feeding larvae (zoea I-III, megalopa) showed high activities of esterases, phosphatases, and exopeptidases indicating the enzymatic ability to utilize endogeneous yolk reserves. SDS-PAGE showed a continuous decrease of proteins or proteids in the range of 59-81 kDa during ontogenetic development from the eggs through the zoeal stages to the first juvenile crab stage, CI. This reduction reflects the degradation of storage compounds during lecithotrophic larval development. Activities of the endopeptidases, trypsin and chymotrypsin, were low in eggs and larvae but increased significantly in the first juvenile crab stage. These enzymes typically facilitate the first steps of proteolysis in the extra-cellular spaces of the midgut gland and in the stomach. Their scarcity indicates that the larvae of L. santolla are physiologically not prepared to digest external food. This ability seems to appear first in the CI stage. Extracts of juvenile midgut glands and the gastric fluids of adults showed high activities of a variety of digestive enzymes including phosphatases, carbohydrases, as well as endo- and exopeptidases. High activities of digestive enzymes in adults may compensate for scarce food supply and rate-limiting low temperatures in the predominantly sub-Antarctic habitats of L. santolla. Fil: Saborowski, R. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; Alemania Fil: Thatje, S. Alfred-Wegener-Institut. Helmholtz-Zentrum für Polar und Meeresforschung; Alemania. University of Southampton; Reino Unido Fil: Calcagno, Javier Ángel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lovrich, Gustavo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones ... |
format |
Article in Journal/Newspaper |
author |
Saborowski, R. Thatje, S. Calcagno, Javier Ángel Lovrich, Gustavo Alejandro Anger, K. |
author_facet |
Saborowski, R. Thatje, S. Calcagno, Javier Ángel Lovrich, Gustavo Alejandro Anger, K. |
author_sort |
Saborowski, R. |
title |
Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla |
title_short |
Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla |
title_full |
Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla |
title_fullStr |
Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla |
title_full_unstemmed |
Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla |
title_sort |
digestive enzymes in the ontogenetic stages of the southern king crab, lithodes santolla |
publisher |
Springer |
url |
http://hdl.handle.net/11336/150639 |
long_lat |
ENVELOPE(-62.917,-62.917,-64.833,-64.833) |
geographic |
Antarctic Argentina Austral Gustavo |
geographic_facet |
Antarctic Argentina Austral Gustavo |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00227-005-0240-x info:eu-repo/semantics/altIdentifier/doi/10.1007/s00227-005-0240-x http://hdl.handle.net/11336/150639 Saborowski, R.; Thatje, S.; Calcagno, Javier Ángel; Lovrich, Gustavo Alejandro; Anger, K.; Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla; Springer; Marine Biology; 149; 4; 7-2006; 865-873 0025-3162 1432-1793 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1007/s00227-005-0240-x |
container_title |
Marine Biology |
container_volume |
149 |
container_issue |
4 |
container_start_page |
865 |
op_container_end_page |
873 |
_version_ |
1779320093916790784 |