The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach
The ability to detect species at low densities, greatly improves the success of management action on alien invasive species and decreases their possible impact on ecosystems. In the last two decades, exotic Chinook salmon (Oncorhynchus tshawytscha) have established populations in both Pacific and At...
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Language: | English |
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ftconicet:oai:ri.conicet.gov.ar:11336/147676 2023-10-09T21:47:20+02:00 The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach Nardi, Cristina Fernanda Fernandez, Daniel Alfredo Vanella, Fabián Alberto Chalde, Tomás application/pdf http://hdl.handle.net/11336/147676 eng eng Springer info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10530-018-1908-8 info:eu-repo/semantics/altIdentifier/doi/10.1007/S10530-018-1908-8 http://hdl.handle.net/11336/147676 Nardi, Cristina Fernanda; Fernandez, Daniel Alfredo; Vanella, Fabián Alberto; Chalde, Tomás; The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach; Springer; Biological Invasions; 21; 4; 1-2019; 1415-1425 1387-3547 CONICET Digital CONICET info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ ALIEN INVASIVE SPECIES EDNA NON-NATIVE SPECIES SALMONIDS SOUTH AMERICA TIERRA DEL FUEGO https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1007/S10530-018-1908-8 2023-09-24T20:20:17Z The ability to detect species at low densities, greatly improves the success of management action on alien invasive species and decreases their possible impact on ecosystems. In the last two decades, exotic Chinook salmon (Oncorhynchus tshawytscha) have established populations in both Pacific and Atlantic river basins of Patagonia. The last established populations have been reported in the extreme south of Patagonia, on the island of Tierra del Fuego (TDF). The relatively recent appearance of Chinook salmon in TDF and the great phenotypic plasticity of this species, make it necessary to study their distribution and expansion as soon as possible, since they have the potential to negatively impact on native ecosystems. With the objective of knowing the current distribution status of exotic Chinook salmon in TDF, we optimized and implemented a detection method based on environmental DNA (eDNA). First, we designed Chinook salmon-specific primers, with no cross-amplification, using DNA from other species that are living at the same environment. Second, we validated the primers in situ by detecting Chinook salmon DNA from natural environments at the same time that we performed a conventional survey using an electrofishing survey method. Finally, we collected water samples from 10 river basins and one estuary within TDF and one river basin from Isla de los Estados (IE) and performed single-species real-time PCR assays. We were able to detect Chinook salmon DNA from 5 basins and from the estuary in TDF. These eDNA-based results allowed us to confirm the expansion of exotic Chinook salmon since they were first reported in TDF. Fil: Nardi, Cristina Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina. Universidad Nacional de Tierra del Fuego, Antártida e Islas del Atlántico Sur. Instituto de Ciencias Polares, Ambientales y Recursos Naturales; Argentina Fil: Fernandez, Daniel Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. ... Article in Journal/Newspaper Antártida Tierra del Fuego CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Austral Patagonia Pacific Argentina Fernandez ENVELOPE(-62.233,-62.233,-63.250,-63.250) Biological Invasions 21 4 1415 1425 |
institution |
Open Polar |
collection |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
op_collection_id |
ftconicet |
language |
English |
topic |
ALIEN INVASIVE SPECIES EDNA NON-NATIVE SPECIES SALMONIDS SOUTH AMERICA TIERRA DEL FUEGO https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
spellingShingle |
ALIEN INVASIVE SPECIES EDNA NON-NATIVE SPECIES SALMONIDS SOUTH AMERICA TIERRA DEL FUEGO https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Nardi, Cristina Fernanda Fernandez, Daniel Alfredo Vanella, Fabián Alberto Chalde, Tomás The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach |
topic_facet |
ALIEN INVASIVE SPECIES EDNA NON-NATIVE SPECIES SALMONIDS SOUTH AMERICA TIERRA DEL FUEGO https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
description |
The ability to detect species at low densities, greatly improves the success of management action on alien invasive species and decreases their possible impact on ecosystems. In the last two decades, exotic Chinook salmon (Oncorhynchus tshawytscha) have established populations in both Pacific and Atlantic river basins of Patagonia. The last established populations have been reported in the extreme south of Patagonia, on the island of Tierra del Fuego (TDF). The relatively recent appearance of Chinook salmon in TDF and the great phenotypic plasticity of this species, make it necessary to study their distribution and expansion as soon as possible, since they have the potential to negatively impact on native ecosystems. With the objective of knowing the current distribution status of exotic Chinook salmon in TDF, we optimized and implemented a detection method based on environmental DNA (eDNA). First, we designed Chinook salmon-specific primers, with no cross-amplification, using DNA from other species that are living at the same environment. Second, we validated the primers in situ by detecting Chinook salmon DNA from natural environments at the same time that we performed a conventional survey using an electrofishing survey method. Finally, we collected water samples from 10 river basins and one estuary within TDF and one river basin from Isla de los Estados (IE) and performed single-species real-time PCR assays. We were able to detect Chinook salmon DNA from 5 basins and from the estuary in TDF. These eDNA-based results allowed us to confirm the expansion of exotic Chinook salmon since they were first reported in TDF. Fil: Nardi, Cristina Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina. Universidad Nacional de Tierra del Fuego, Antártida e Islas del Atlántico Sur. Instituto de Ciencias Polares, Ambientales y Recursos Naturales; Argentina Fil: Fernandez, Daniel Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. ... |
format |
Article in Journal/Newspaper |
author |
Nardi, Cristina Fernanda Fernandez, Daniel Alfredo Vanella, Fabián Alberto Chalde, Tomás |
author_facet |
Nardi, Cristina Fernanda Fernandez, Daniel Alfredo Vanella, Fabián Alberto Chalde, Tomás |
author_sort |
Nardi, Cristina Fernanda |
title |
The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach |
title_short |
The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach |
title_full |
The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach |
title_fullStr |
The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach |
title_full_unstemmed |
The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach |
title_sort |
expansion of exotic chinook salmon (oncorhynchus tshawytscha) in the extreme south of patagonia: an environmental dna approach |
publisher |
Springer |
url |
http://hdl.handle.net/11336/147676 |
long_lat |
ENVELOPE(-62.233,-62.233,-63.250,-63.250) |
geographic |
Austral Patagonia Pacific Argentina Fernandez |
geographic_facet |
Austral Patagonia Pacific Argentina Fernandez |
genre |
Antártida Tierra del Fuego |
genre_facet |
Antártida Tierra del Fuego |
op_relation |
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10530-018-1908-8 info:eu-repo/semantics/altIdentifier/doi/10.1007/S10530-018-1908-8 http://hdl.handle.net/11336/147676 Nardi, Cristina Fernanda; Fernandez, Daniel Alfredo; Vanella, Fabián Alberto; Chalde, Tomás; The expansion of exotic Chinook salmon (Oncorhynchus tshawytscha) in the extreme south of Patagonia: an environmental DNA approach; Springer; Biological Invasions; 21; 4; 1-2019; 1415-1425 1387-3547 CONICET Digital CONICET |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1007/S10530-018-1908-8 |
container_title |
Biological Invasions |
container_volume |
21 |
container_issue |
4 |
container_start_page |
1415 |
op_container_end_page |
1425 |
_version_ |
1779310422174728192 |