Evidence of pathogen-induced immunogenetic selection across the large geographic range of a wild seabird

• Published in: Molecular Biology and Evolution
• Main Authors: Levy, Hila, Fiddaman, Steven R., Vianna, Juliana A., Noll, Daly, Clucas, Gemma V., Sidhu, Jasmine K.H., Polito, Michael J., Bost, Charles A., Phillips, Richard A., Crofts, Sarah, Miller, Gary D., Pistorius, Pierre, Bonnadonna, Francesco, Le Bohec, Celine, Barbosa, Andres, Trathan, Phil, Raya Rey, Andrea Nélida, Frantz, Laurent A.F., Hart, Tom, Smith, Adrian L.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Oxford University Press
• Subjects: ANTARCTICA AND SOUTHERN OCEAN; GENTOO PENGUIN; IMMUNOGENETICS; PATHOGEN-MEDIATED SELECTION; POSITIVE SELECTION; TOLL-LIKE RECEPTORS; https://purl.org/becyt/ford/1.6; https://purl.org/becyt/ford/1; Southern Ocean; Levy; Daly; Antarc*; Antarctica; Gentoo penguin; Pygoscelis papua
• Online Access: http://hdl.handle.net/11336/141119

Over evolutionary time,pathogen challenge shapes theimmunephenotype of the host tobetterrespondtoanincipient threat. The extent and direction of this selection pressure depend on the local pathogen composition, which is in turn determined by biotic and abiotic features of the environment. However, little is known about adaptation to local pathogen threats in wild animals. The Gentoo penguin (Pygoscelis papua) is a species complex that lends itself to the study of immune adaptation becauseof its circumpolardistributionover a large latitudinal range, with littleornoadmixturebetweendifferent clades. Inthis study,we examine thediversity ina key family of innateimmunegenes-theToll-like receptors (TLRs)-across the range of the Gentoo penguin. The three TLRs that we investigated present varying levels of diversity, with TLR4 and TLR5 greatly exceeding the diversity of TLR7.We present evidence of positive selection in TLR4 and TLR5,which points to pathogen-driven adaptation to the local pathogen milieu. Finally, we demonstrate that two positively selected cosegregating sites in TLR5 are sufficient to alter the responsiveness of the receptor to its bacterial ligand, flagellin. Taken together, these results suggest that Gentoo penguins have experienced distinct pathogen-driven selection pressures in different environments, which may be important given the role of the Gentoo penguin as a sentinel species in some of the world's most rapidly changing environments. Fil: Levy, Hila. University of Oxford; Reino Unido Fil: Fiddaman, Steven R. University of Oxford; Reino Unido Fil: Vianna, Juliana A. Pontificia Universidad Católica de Chile; Chile. Universidad Católica de Chile; Chile Fil: Noll, Daly. Pontificia Universidad Católica de Chile; Chile. Universidad de Chile; Chile Fil: Clucas, Gemma V. Cornell University; Estados Unidos Fil: Sidhu, Jasmine K.H. University of Oxford; Reino Unido Fil: Polito, Michael J. Louisiana State University; Estados Unidos Fil: Bost, Charles A. Centre D'etudes Biologiques de ...