Development of a magnetic biocatalyst useful for the synthesis of ethyloleate
Candida antarctica Lipase B was successfully immobilized on magnetite (Fe3O4) nanoparticles functionalized with chitosan and glutaraldehyde. The obtained magnetic catalyst was characterized and its performance was evaluated in solvent-free synthesis of ethyl oleate at room temperature. The performan...
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ftconicet:oai:ri.conicet.gov.ar:11336/13513 2023-10-09T21:46:35+02:00 Development of a magnetic biocatalyst useful for the synthesis of ethyloleate Nicolás, Paula Lassalle, Verónica Leticia Ferreira, Maria Lujan application/pdf http://hdl.handle.net/11336/13513 eng eng Springer info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00449-013-1010-7 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00449-013-1010-7 http://hdl.handle.net/11336/13513 Nicolás, Paula; Lassalle, Verónica Leticia; Ferreira, Maria Lujan; Development of a magnetic biocatalyst useful for the synthesis of ethyloleate; Springer; Bioprocess And Biosystems Engineering; 37; 3; 3-2014; 585-591 1615-7591 1615-7605 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ Magnetic Nanoparticles Chitosan Glutaraldehyde Oleic Acid Candida Antartica Lipase B https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1007/s00449-013-1010-7 2023-09-24T20:04:50Z Candida antarctica Lipase B was successfully immobilized on magnetite (Fe3O4) nanoparticles functionalized with chitosan and glutaraldehyde. The obtained magnetic catalyst was characterized and its performance was evaluated in solvent-free synthesis of ethyl oleate at room temperature. The performance of this biocatalyst was compared with the commercial Novozym 435, as a tool to estimate the efficiency of immobilization. It was found that using 33 mg of the biocatalyst it was possible to reach almost the same activity that was obtained using 12 mg of Novozym 435. Furthermore, this new biocatalyst presents the advantages of not being degraded by short alcohols, being easily recovered from the reaction media by magnetic decantation, and low fabrication cost. The possibility of reutilization was also studied, keeping a significant activity up to eight cycles. A special sampling protocol was also developed for the multiphasic reaction system, to assure accurate results. This novel biocatalyst is an interesting alternative for potential industrial applications, considering the above-mentioned advantages. Fil: Nicolás, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; Argentina Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Universidad Nacional del Sur; Argentina Fil: Ferreira, Maria Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; Argentina Article in Journal/Newspaper Antarc* Antarctica antartic* CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Argentina Ferreira ENVELOPE(-62.050,-62.050,-64.600,-64.600) Bioprocess and Biosystems Engineering 37 3 585 591 |
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CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
op_collection_id |
ftconicet |
language |
English |
topic |
Magnetic Nanoparticles Chitosan Glutaraldehyde Oleic Acid Candida Antartica Lipase B https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
spellingShingle |
Magnetic Nanoparticles Chitosan Glutaraldehyde Oleic Acid Candida Antartica Lipase B https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 Nicolás, Paula Lassalle, Verónica Leticia Ferreira, Maria Lujan Development of a magnetic biocatalyst useful for the synthesis of ethyloleate |
topic_facet |
Magnetic Nanoparticles Chitosan Glutaraldehyde Oleic Acid Candida Antartica Lipase B https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
description |
Candida antarctica Lipase B was successfully immobilized on magnetite (Fe3O4) nanoparticles functionalized with chitosan and glutaraldehyde. The obtained magnetic catalyst was characterized and its performance was evaluated in solvent-free synthesis of ethyl oleate at room temperature. The performance of this biocatalyst was compared with the commercial Novozym 435, as a tool to estimate the efficiency of immobilization. It was found that using 33 mg of the biocatalyst it was possible to reach almost the same activity that was obtained using 12 mg of Novozym 435. Furthermore, this new biocatalyst presents the advantages of not being degraded by short alcohols, being easily recovered from the reaction media by magnetic decantation, and low fabrication cost. The possibility of reutilization was also studied, keeping a significant activity up to eight cycles. A special sampling protocol was also developed for the multiphasic reaction system, to assure accurate results. This novel biocatalyst is an interesting alternative for potential industrial applications, considering the above-mentioned advantages. Fil: Nicolás, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; Argentina Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Universidad Nacional del Sur; Argentina Fil: Ferreira, Maria Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; Argentina |
format |
Article in Journal/Newspaper |
author |
Nicolás, Paula Lassalle, Verónica Leticia Ferreira, Maria Lujan |
author_facet |
Nicolás, Paula Lassalle, Verónica Leticia Ferreira, Maria Lujan |
author_sort |
Nicolás, Paula |
title |
Development of a magnetic biocatalyst useful for the synthesis of ethyloleate |
title_short |
Development of a magnetic biocatalyst useful for the synthesis of ethyloleate |
title_full |
Development of a magnetic biocatalyst useful for the synthesis of ethyloleate |
title_fullStr |
Development of a magnetic biocatalyst useful for the synthesis of ethyloleate |
title_full_unstemmed |
Development of a magnetic biocatalyst useful for the synthesis of ethyloleate |
title_sort |
development of a magnetic biocatalyst useful for the synthesis of ethyloleate |
publisher |
Springer |
url |
http://hdl.handle.net/11336/13513 |
long_lat |
ENVELOPE(-62.050,-62.050,-64.600,-64.600) |
geographic |
Argentina Ferreira |
geographic_facet |
Argentina Ferreira |
genre |
Antarc* Antarctica antartic* |
genre_facet |
Antarc* Antarctica antartic* |
op_relation |
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00449-013-1010-7 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00449-013-1010-7 http://hdl.handle.net/11336/13513 Nicolás, Paula; Lassalle, Verónica Leticia; Ferreira, Maria Lujan; Development of a magnetic biocatalyst useful for the synthesis of ethyloleate; Springer; Bioprocess And Biosystems Engineering; 37; 3; 3-2014; 585-591 1615-7591 1615-7605 |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1007/s00449-013-1010-7 |
container_title |
Bioprocess and Biosystems Engineering |
container_volume |
37 |
container_issue |
3 |
container_start_page |
585 |
op_container_end_page |
591 |
_version_ |
1779322317564805120 |