First detection of Ostreid herpesvirus 1 in wild Crassostrea gigas in Argentina

• Published in: Journal of Invertebrate Pathology
• Main Authors: Barbieri, Elena Susana, Medina, Cintia Débora, Vázquez, Nuria Natalia, Fiorito, Carla Daniela, Martelli, Antonela, Wigdorovitz, Andrés, Schwindt, Evangelina, Morga, Benjamín, Renault, Tristan, Parreño, Viviana, Barón, Pedro J.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Academic Press Inc Elsevier Science
• Subjects: OSTREID HERPESVIRUS 1; CRASSOSTREA GIGAS; PACIFIC OYSTER; ARGENTINA; https://purl.org/becyt/ford/4.3; https://purl.org/becyt/ford/4; Pacific; Argentina; Medina; Crassostrea gigas; Pacific oyster
• Online Access: http://hdl.handle.net/11336/119654

Ostreid herpesvirus 1 (OsHV-1) is a DNA virus of the genus Ostreavirus (Malacoherpesviridae family, Herpesvirales order). Worldwide, OsHV-1 and its microvariants have been associated with increased mortality of Pacific oysters, Crassostrea gigas. Adult asymptomatic oysters also have shown a high prevalence of viral infection. As a consequence, surveillance is needed to better describe OsHV-1 diversity, pathogenicity, clinical signs, and geographical distribution. We examined Crassostrea gigas sampled in October 2017 from the inner zone of the Bahía Blanca Estuary, Argentina, and found that 8 of 30 specimens (26.7%) presented macroscopic lesions in mantle tissues. Histological analysis revealed abnormal presentation of mantle epithelial cells and connective tissues. Conventional and real-time PCR conducted on the oyster samples revealed 70% to be positive for presence of OsHV-1 DNA. The nucleotide sequence of the amplicon obtained from one sample using the primer pair IA1/IA2 (targeting ORF 42/43) was 99% identical to OsHV-1 reference as well as µVar strains B and A (KY271630, KY242785.1), sequenced from France and Ireland. This finding represents the first detection of OsHV-1 DNA in a wild population of C. gigas in Argentina in association with gross mantle lesions. Fil: Barbieri, Elena Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina Fil: Medina, Cintia Débora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina Fil: Vázquez, Nuria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina Fil: Fiorito, Carla Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico ...