Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942)
Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecul...
Published in: | Journal of Helminthology |
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Main Authors: | , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Cambridge University Press
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Online Access: | http://hdl.handle.net/11336/10760 |
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CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
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ftconicet |
language |
English |
topic |
Stegophorus macronectes Pygoscelis antarctica Penguins Antarctica https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
spellingShingle |
Stegophorus macronectes Pygoscelis antarctica Penguins Antarctica https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Vidal, Virginia Ortiz, Juana Diaz, Julia Inés Zafrilla, Basilio Bonete, Maria José Ruíz de Ybañez, Maria Rocío Palacios, Maria José Benzal, Jesús Valera, Francisco de la Cruz, Carlos Motas, Miguel Bautista, Vanesa Machordom, Annie Barbosa, Andrés Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
topic_facet |
Stegophorus macronectes Pygoscelis antarctica Penguins Antarctica https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
description |
Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecular data on this previously poorly described parasite. Nuclear rDNA sequences (18S, 5.8S, 28S and internal transcribed spacer (ITS) regions) were isolated from S. macronectes specimens collected from the chinstrap penguin Pygoscelis antarctica Forster on Deception Island, Antarctica. Using 18S rDNA sequences, phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian inference) of the order Spirurida were performed to determine the phylogenetic location of this species. Primer pairs of the ITS regions were designed for genus-level identification of specimens, regardless of their cycle, as an alternative to coprological methods. The utility of this molecular method for identification of morphologically altered specimens is also discussed. Fil: Vidal, Virginia. Universidad de Murcia; España. Consejo Superior de Investigaciones Cientificas; España. Museo Nacional de Ciencias Naturales. Departamento de Ecología Evolutiva; España Fil: Ortiz, Juana. Universidad de Murcia; España. Consejo Superior de Investigaciones Cientificas; España Fil: Diaz, Julia Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina Fil: Zafrilla, Basilio. Universidad de Alicante. Facultad de Ciencias; España Fil: Bonete, Maria José. Universidad de Alicante. Facultad de Ciencias; España Fil: Ruíz de Ybañez, Maria Rocío. Universidad de Murcia; España. Consejo Superior de Investigaciones Cientificas; España Fil: Palacios, Maria José. Consejo Superior de Investigaciones Cientificas. Estacion Experimental de Zonas Aridas; España ... |
format |
Article in Journal/Newspaper |
author |
Vidal, Virginia Ortiz, Juana Diaz, Julia Inés Zafrilla, Basilio Bonete, Maria José Ruíz de Ybañez, Maria Rocío Palacios, Maria José Benzal, Jesús Valera, Francisco de la Cruz, Carlos Motas, Miguel Bautista, Vanesa Machordom, Annie Barbosa, Andrés |
author_facet |
Vidal, Virginia Ortiz, Juana Diaz, Julia Inés Zafrilla, Basilio Bonete, Maria José Ruíz de Ybañez, Maria Rocío Palacios, Maria José Benzal, Jesús Valera, Francisco de la Cruz, Carlos Motas, Miguel Bautista, Vanesa Machordom, Annie Barbosa, Andrés |
author_sort |
Vidal, Virginia |
title |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_short |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_full |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_fullStr |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_full_unstemmed |
Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) |
title_sort |
morphological, molecular and phylogenetic analyses of the spirurid nematode stegophorus macronectes (johnston & mawson, 1942) |
publisher |
Cambridge University Press |
url |
http://hdl.handle.net/11336/10760 |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) ENVELOPE(-59.717,-59.717,-62.450,-62.450) ENVELOPE(-60.667,-60.667,-63.783,-63.783) ENVELOPE(-63.283,-63.283,-64.383,-64.383) ENVELOPE(-62.450,-62.450,-64.867,-64.867) |
geographic |
Antarctic Argentina Deception Island Ortiz Diaz Bonete Vidal |
geographic_facet |
Antarctic Argentina Deception Island Ortiz Diaz Bonete Vidal |
genre |
Antarc* Antarctic Antarctica Chinstrap penguin Deception Island Pygoscelis antarctica |
genre_facet |
Antarc* Antarctic Antarctica Chinstrap penguin Deception Island Pygoscelis antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1017/S0022149X15000218 info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/journal-of-helminthology/article/div-classtitlemorphological-molecular-and-phylogenetic-analyses-of-the-spirurid-nematode-span-classitalicstegophorus-macronectesspan-johnston-andamp-mawson-1942div/8271DEAB57B35673D36A020179E5F606 http://hdl.handle.net/11336/10760 Vidal, Virginia; Ortiz, Juana; Diaz, Julia Inés; Zafrilla, Basilio; Bonete, Maria José; et al.; Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942); Cambridge University Press; Journal Of Helminthology; 90; 2; 3-2016; 214-222 0022-149X 1475-2697 |
op_rights |
info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
op_doi |
https://doi.org/10.1017/S0022149X15000218 |
container_title |
Journal of Helminthology |
container_volume |
90 |
container_issue |
2 |
container_start_page |
214 |
op_container_end_page |
222 |
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1779310176618151936 |
spelling |
ftconicet:oai:ri.conicet.gov.ar:11336/10760 2023-10-09T21:47:14+02:00 Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942) Vidal, Virginia Ortiz, Juana Diaz, Julia Inés Zafrilla, Basilio Bonete, Maria José Ruíz de Ybañez, Maria Rocío Palacios, Maria José Benzal, Jesús Valera, Francisco de la Cruz, Carlos Motas, Miguel Bautista, Vanesa Machordom, Annie Barbosa, Andrés application/pdf http://hdl.handle.net/11336/10760 eng eng Cambridge University Press info:eu-repo/semantics/altIdentifier/doi/10.1017/S0022149X15000218 info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/journal-of-helminthology/article/div-classtitlemorphological-molecular-and-phylogenetic-analyses-of-the-spirurid-nematode-span-classitalicstegophorus-macronectesspan-johnston-andamp-mawson-1942div/8271DEAB57B35673D36A020179E5F606 http://hdl.handle.net/11336/10760 Vidal, Virginia; Ortiz, Juana; Diaz, Julia Inés; Zafrilla, Basilio; Bonete, Maria José; et al.; Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942); Cambridge University Press; Journal Of Helminthology; 90; 2; 3-2016; 214-222 0022-149X 1475-2697 info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ Stegophorus macronectes Pygoscelis antarctica Penguins Antarctica https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion ftconicet https://doi.org/10.1017/S0022149X15000218 2023-09-24T19:13:44Z Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecular data on this previously poorly described parasite. Nuclear rDNA sequences (18S, 5.8S, 28S and internal transcribed spacer (ITS) regions) were isolated from S. macronectes specimens collected from the chinstrap penguin Pygoscelis antarctica Forster on Deception Island, Antarctica. Using 18S rDNA sequences, phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian inference) of the order Spirurida were performed to determine the phylogenetic location of this species. Primer pairs of the ITS regions were designed for genus-level identification of specimens, regardless of their cycle, as an alternative to coprological methods. The utility of this molecular method for identification of morphologically altered specimens is also discussed. Fil: Vidal, Virginia. Universidad de Murcia; España. Consejo Superior de Investigaciones Cientificas; España. Museo Nacional de Ciencias Naturales. Departamento de Ecología Evolutiva; España Fil: Ortiz, Juana. Universidad de Murcia; España. Consejo Superior de Investigaciones Cientificas; España Fil: Diaz, Julia Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina Fil: Zafrilla, Basilio. Universidad de Alicante. Facultad de Ciencias; España Fil: Bonete, Maria José. Universidad de Alicante. Facultad de Ciencias; España Fil: Ruíz de Ybañez, Maria Rocío. Universidad de Murcia; España. Consejo Superior de Investigaciones Cientificas; España Fil: Palacios, Maria José. Consejo Superior de Investigaciones Cientificas. Estacion Experimental de Zonas Aridas; España ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Chinstrap penguin Deception Island Pygoscelis antarctica CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) Antarctic Argentina Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Ortiz ENVELOPE(-59.717,-59.717,-62.450,-62.450) Diaz ENVELOPE(-60.667,-60.667,-63.783,-63.783) Bonete ENVELOPE(-63.283,-63.283,-64.383,-64.383) Vidal ENVELOPE(-62.450,-62.450,-64.867,-64.867) Journal of Helminthology 90 2 214 222 |