Melt procedure affects the photosynthetic response of sea ice algae.
The accuracy of sea ice algal production estimates is influenced by the range of melting procedures used in studies to obtain a liquid sample for incubation, particularly in relation to the duration of melt and the approach to buffering for osmotic shock. In this research, ice algal photophysiology...
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ftcolumbiauniv:oai:academiccommons.columbia.edu:10.7916/tvh7-1d96 2023-10-09T21:49:20+02:00 Melt procedure affects the photosynthetic response of sea ice algae. Campbell, Karley Mundy, C. J. Juhl, Andrew R. Dalman, Laura A. Michel, Christine Galley, Ryan J. Else, Brent E. Geilfus, Nicolas X. Rysgaard, Søren 2019 https://doi.org/10.7916/tvh7-1d96 English eng https://doi.org/10.7916/tvh7-1d96 Sea ice Marine algae Photosynthesis Research--Methodology Articles 2019 ftcolumbiauniv https://doi.org/10.7916/tvh7-1d96 2023-09-09T22:20:51Z The accuracy of sea ice algal production estimates is influenced by the range of melting procedures used in studies to obtain a liquid sample for incubation, particularly in relation to the duration of melt and the approach to buffering for osmotic shock. In this research, ice algal photophysiology from 14C incubations was compared in field samples prepared by three melt procedures: (i) a rapid 4 h melt of the bottommost ( < 1 cm) ice algal layer scraped into a large volume of filtered seawater (salinity 27–30), (ii) melt of a bottom 5 cm section diluted into a moderate volume of filtered seawater over 24 h (salinity 20–24), and (iii) melt of a bottom 5 cm section without any filtered seawater dilution over about 48 h (salinity 10–12). Maximum photosynthetic rate, photosynthetic efficiency and production at zero irradiance were significantly affected by the melt treatment employed in experiments. All variables were greatest in the highly diluted scrape sample and lowest in the bulk-ice samples melted in the absence of filtered seawater. Laboratory experiments exposing cultures of the common sea ice diatom Nitzschia frigida to different salinities and light conditions suggested that the field-based responses can be attributed to the rapid ( < 4 h) adverse effects of exposing cells to low salinities during melt without dilution. The observed differences in primary production between melt treatments were estimated to account for over 60% of the variability in production estimates reported for the Arctic. Future studies are strongly encouraged to replicate salinity conditions representative of in situ values during the melting process to minimize hypoosmotic stress, thereby most accurately estimating primary production. Article in Journal/Newspaper Arctic ice algae Sea ice Columbia University: Academic Commons Arctic |
institution |
Open Polar |
collection |
Columbia University: Academic Commons |
op_collection_id |
ftcolumbiauniv |
language |
English |
topic |
Sea ice Marine algae Photosynthesis Research--Methodology |
spellingShingle |
Sea ice Marine algae Photosynthesis Research--Methodology Campbell, Karley Mundy, C. J. Juhl, Andrew R. Dalman, Laura A. Michel, Christine Galley, Ryan J. Else, Brent E. Geilfus, Nicolas X. Rysgaard, Søren Melt procedure affects the photosynthetic response of sea ice algae. |
topic_facet |
Sea ice Marine algae Photosynthesis Research--Methodology |
description |
The accuracy of sea ice algal production estimates is influenced by the range of melting procedures used in studies to obtain a liquid sample for incubation, particularly in relation to the duration of melt and the approach to buffering for osmotic shock. In this research, ice algal photophysiology from 14C incubations was compared in field samples prepared by three melt procedures: (i) a rapid 4 h melt of the bottommost ( < 1 cm) ice algal layer scraped into a large volume of filtered seawater (salinity 27–30), (ii) melt of a bottom 5 cm section diluted into a moderate volume of filtered seawater over 24 h (salinity 20–24), and (iii) melt of a bottom 5 cm section without any filtered seawater dilution over about 48 h (salinity 10–12). Maximum photosynthetic rate, photosynthetic efficiency and production at zero irradiance were significantly affected by the melt treatment employed in experiments. All variables were greatest in the highly diluted scrape sample and lowest in the bulk-ice samples melted in the absence of filtered seawater. Laboratory experiments exposing cultures of the common sea ice diatom Nitzschia frigida to different salinities and light conditions suggested that the field-based responses can be attributed to the rapid ( < 4 h) adverse effects of exposing cells to low salinities during melt without dilution. The observed differences in primary production between melt treatments were estimated to account for over 60% of the variability in production estimates reported for the Arctic. Future studies are strongly encouraged to replicate salinity conditions representative of in situ values during the melting process to minimize hypoosmotic stress, thereby most accurately estimating primary production. |
format |
Article in Journal/Newspaper |
author |
Campbell, Karley Mundy, C. J. Juhl, Andrew R. Dalman, Laura A. Michel, Christine Galley, Ryan J. Else, Brent E. Geilfus, Nicolas X. Rysgaard, Søren |
author_facet |
Campbell, Karley Mundy, C. J. Juhl, Andrew R. Dalman, Laura A. Michel, Christine Galley, Ryan J. Else, Brent E. Geilfus, Nicolas X. Rysgaard, Søren |
author_sort |
Campbell, Karley |
title |
Melt procedure affects the photosynthetic response of sea ice algae. |
title_short |
Melt procedure affects the photosynthetic response of sea ice algae. |
title_full |
Melt procedure affects the photosynthetic response of sea ice algae. |
title_fullStr |
Melt procedure affects the photosynthetic response of sea ice algae. |
title_full_unstemmed |
Melt procedure affects the photosynthetic response of sea ice algae. |
title_sort |
melt procedure affects the photosynthetic response of sea ice algae. |
publishDate |
2019 |
url |
https://doi.org/10.7916/tvh7-1d96 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic ice algae Sea ice |
genre_facet |
Arctic ice algae Sea ice |
op_relation |
https://doi.org/10.7916/tvh7-1d96 |
op_doi |
https://doi.org/10.7916/tvh7-1d96 |
_version_ |
1779312349302226944 |