Dissolved organic phosphorus utilization by phytoplankton reveals preferential degradation of polyphosphates over phosphomonoesters
The nutritionally available pool of dissolved organic phosphorus (DOP) supports marine primary productivity in a range of ocean ecosystems but remains poorly resolved. Here, the relative lability of model phosphorus (P) compounds representing the major P(V) bond classes of marine DOP – phosphomonoes...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2018
|
Subjects: | |
Online Access: | https://doi.org/10.7916/d8-crnq-4z64 |
id |
ftcolumbiauniv:oai:academiccommons.columbia.edu:10.7916/d8-crnq-4z64 |
---|---|
record_format |
openpolar |
spelling |
ftcolumbiauniv:oai:academiccommons.columbia.edu:10.7916/d8-crnq-4z64 2023-05-15T17:31:37+02:00 Dissolved organic phosphorus utilization by phytoplankton reveals preferential degradation of polyphosphates over phosphomonoesters Diaz, Julia M. Holland, Alisia Sanders, James G. Bulski, Karrie Mollett, Douglas Chou, Chau-Wen Phillips, Dennis Tang, Yuanzhi Duhamel, Solange 2018 https://doi.org/10.7916/d8-crnq-4z64 English eng https://doi.org/10.7916/d8-crnq-4z64 Geochemistry Phytoplankton Organophosphorus compounds Polyphosphates Phosphorus in animal nutrition articles 2018 ftcolumbiauniv https://doi.org/10.7916/d8-crnq-4z64 2019-04-04T08:18:19Z The nutritionally available pool of dissolved organic phosphorus (DOP) supports marine primary productivity in a range of ocean ecosystems but remains poorly resolved. Here, the relative lability of model phosphorus (P) compounds representing the major P(V) bond classes of marine DOP – phosphomonoesters (P-O-C) and phosphoanhydrides (P-O-P) – was assessed in diatom cultures of the genus Thalassiosira, as well as coastal field sites of the western North Atlantic. In diatom samples, maximum enzymatic hydrolysis rates revealed that the P-anhydride bonds of inorganic tripolyphosphate (3poly-P), followed by the P-anhydride bonds of adenosine 5′-triphosphate (ATP), were preferentially degraded relative to the P-monoesters adenosine 5′-monophosphate (AMP) and 4-methylumbelliferone phosphate (MUF-P). Consistent with these rate measurements, targeted proteomics analysis demonstrated that the underlying phosphatase diversity present in diatom samples was dominated by P-anhydride degrading enzymes (inorganic pyrophosphatases and nucleoside triphosphatases). Furthermore, biomass-normalized rates of ATP degradation were always suppressed under P-replete conditions in diatom cultures, but the effect of overall P availability on 3poly-P degradation was inconsistent among diatom strains, suggesting that inorganic polyphosphate (poly-P) degradation may persist irrespective of prevailing P levels in the marine environment. Indeed, the majority of field sites examined in the P-replete coastal western North Atlantic exhibited significantly higher maximum rates of inorganic poly-P hydrolysis relative to P-monoester hydrolysis, which was largely driven by phytoplankton dynamics. Based on these results, the possibility that P-anhydride utilization may contribute comparably or even more substantially than P-esters to community-level P demand, phytoplankton growth, and primary productivity should be considered. Article in Journal/Newspaper North Atlantic Columbia University: Academic Commons |
institution |
Open Polar |
collection |
Columbia University: Academic Commons |
op_collection_id |
ftcolumbiauniv |
language |
English |
topic |
Geochemistry Phytoplankton Organophosphorus compounds Polyphosphates Phosphorus in animal nutrition |
spellingShingle |
Geochemistry Phytoplankton Organophosphorus compounds Polyphosphates Phosphorus in animal nutrition Diaz, Julia M. Holland, Alisia Sanders, James G. Bulski, Karrie Mollett, Douglas Chou, Chau-Wen Phillips, Dennis Tang, Yuanzhi Duhamel, Solange Dissolved organic phosphorus utilization by phytoplankton reveals preferential degradation of polyphosphates over phosphomonoesters |
topic_facet |
Geochemistry Phytoplankton Organophosphorus compounds Polyphosphates Phosphorus in animal nutrition |
description |
The nutritionally available pool of dissolved organic phosphorus (DOP) supports marine primary productivity in a range of ocean ecosystems but remains poorly resolved. Here, the relative lability of model phosphorus (P) compounds representing the major P(V) bond classes of marine DOP – phosphomonoesters (P-O-C) and phosphoanhydrides (P-O-P) – was assessed in diatom cultures of the genus Thalassiosira, as well as coastal field sites of the western North Atlantic. In diatom samples, maximum enzymatic hydrolysis rates revealed that the P-anhydride bonds of inorganic tripolyphosphate (3poly-P), followed by the P-anhydride bonds of adenosine 5′-triphosphate (ATP), were preferentially degraded relative to the P-monoesters adenosine 5′-monophosphate (AMP) and 4-methylumbelliferone phosphate (MUF-P). Consistent with these rate measurements, targeted proteomics analysis demonstrated that the underlying phosphatase diversity present in diatom samples was dominated by P-anhydride degrading enzymes (inorganic pyrophosphatases and nucleoside triphosphatases). Furthermore, biomass-normalized rates of ATP degradation were always suppressed under P-replete conditions in diatom cultures, but the effect of overall P availability on 3poly-P degradation was inconsistent among diatom strains, suggesting that inorganic polyphosphate (poly-P) degradation may persist irrespective of prevailing P levels in the marine environment. Indeed, the majority of field sites examined in the P-replete coastal western North Atlantic exhibited significantly higher maximum rates of inorganic poly-P hydrolysis relative to P-monoester hydrolysis, which was largely driven by phytoplankton dynamics. Based on these results, the possibility that P-anhydride utilization may contribute comparably or even more substantially than P-esters to community-level P demand, phytoplankton growth, and primary productivity should be considered. |
format |
Article in Journal/Newspaper |
author |
Diaz, Julia M. Holland, Alisia Sanders, James G. Bulski, Karrie Mollett, Douglas Chou, Chau-Wen Phillips, Dennis Tang, Yuanzhi Duhamel, Solange |
author_facet |
Diaz, Julia M. Holland, Alisia Sanders, James G. Bulski, Karrie Mollett, Douglas Chou, Chau-Wen Phillips, Dennis Tang, Yuanzhi Duhamel, Solange |
author_sort |
Diaz, Julia M. |
title |
Dissolved organic phosphorus utilization by phytoplankton reveals preferential degradation of polyphosphates over phosphomonoesters |
title_short |
Dissolved organic phosphorus utilization by phytoplankton reveals preferential degradation of polyphosphates over phosphomonoesters |
title_full |
Dissolved organic phosphorus utilization by phytoplankton reveals preferential degradation of polyphosphates over phosphomonoesters |
title_fullStr |
Dissolved organic phosphorus utilization by phytoplankton reveals preferential degradation of polyphosphates over phosphomonoesters |
title_full_unstemmed |
Dissolved organic phosphorus utilization by phytoplankton reveals preferential degradation of polyphosphates over phosphomonoesters |
title_sort |
dissolved organic phosphorus utilization by phytoplankton reveals preferential degradation of polyphosphates over phosphomonoesters |
publishDate |
2018 |
url |
https://doi.org/10.7916/d8-crnq-4z64 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
https://doi.org/10.7916/d8-crnq-4z64 |
op_doi |
https://doi.org/10.7916/d8-crnq-4z64 |
_version_ |
1766129295974989824 |