PCR-based sex determination of cetaceans and dugong from the Indian seas
A sex-determination technique based on PCR amplifi- cation of genomic DNA extracted from the skin tissue has been standardized in cetaceans and dugong sam-pled from the Indian seas. A Y-chromosome-specific region (SRY or Sex-determining Y-chromosome gene) of 210–224 bp size in the genome has been am...
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ftcmfri:oai:eprints.cmfri.org.in:2 2023-05-15T15:36:22+02:00 PCR-based sex determination of cetaceans and dugong from the Indian seas Jayasankar, P Anoop, B Rajagopalan, M 2008 application/pdf http://eprints.cmfri.org.in/2/ http://eprints.cmfri.org.in/2/1/1513.pdf http://www.ias.ac.in/currsci/jun102008/1513.pdf en eng Current Science Association http://eprints.cmfri.org.in/2/1/1513.pdf Jayasankar, P and Anoop, B and Rajagopalan, M (2008) PCR-based sex determination of cetaceans and dugong from the Indian seas. Current Science, 94 (11). pp. 1513-1516. Fish Genetics Article PeerReviewed 2008 ftcmfri 2021-01-24T16:17:37Z A sex-determination technique based on PCR amplifi- cation of genomic DNA extracted from the skin tissue has been standardized in cetaceans and dugong sam-pled from the Indian seas. A Y-chromosome-specific region (SRY or Sex-determining Y-chromosome gene) of 210–224 bp size in the genome has been amplified (only in males) using specific PCR primers. A fragment of the ZFX/ZFY (zinc finger protein genes located both on the X and Y chromosomes respectively) re-gion in the size range 442–445 bp is also amplified (in both sexes) using another pair of primers simultaneously as positive controls for confirmation of sex. Molecular sexing was standardized in spinner dolphin (Stenella longirostris), bridled dolphin (Stenella attenuata), bottlenose dolphin (Tursiops aduncus), Indo-Pacific humpbacked dolphin (Sousa chinensis), Risso’s dolphin (Grampus griseus), finless porpoise (Neopho-caena phocaenoides), sperm whale (Physeter macro-cephalus), blue whale (Balaenoptera musculus), Bryde’s whale (Balaenoptera edeni) and dugong (Dugong du-gon), which are all vulnerable/endangered species pro- tected under the Indian Wildlife Act. Article in Journal/Newspaper Balaenoptera musculus Blue whale Sperm whale Central Marine Fisheries Research Institute, Kochi, India: Eprints@CMFRI Indian Pacific |
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Open Polar |
collection |
Central Marine Fisheries Research Institute, Kochi, India: Eprints@CMFRI |
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ftcmfri |
language |
English |
topic |
Fish Genetics |
spellingShingle |
Fish Genetics Jayasankar, P Anoop, B Rajagopalan, M PCR-based sex determination of cetaceans and dugong from the Indian seas |
topic_facet |
Fish Genetics |
description |
A sex-determination technique based on PCR amplifi- cation of genomic DNA extracted from the skin tissue has been standardized in cetaceans and dugong sam-pled from the Indian seas. A Y-chromosome-specific region (SRY or Sex-determining Y-chromosome gene) of 210–224 bp size in the genome has been amplified (only in males) using specific PCR primers. A fragment of the ZFX/ZFY (zinc finger protein genes located both on the X and Y chromosomes respectively) re-gion in the size range 442–445 bp is also amplified (in both sexes) using another pair of primers simultaneously as positive controls for confirmation of sex. Molecular sexing was standardized in spinner dolphin (Stenella longirostris), bridled dolphin (Stenella attenuata), bottlenose dolphin (Tursiops aduncus), Indo-Pacific humpbacked dolphin (Sousa chinensis), Risso’s dolphin (Grampus griseus), finless porpoise (Neopho-caena phocaenoides), sperm whale (Physeter macro-cephalus), blue whale (Balaenoptera musculus), Bryde’s whale (Balaenoptera edeni) and dugong (Dugong du-gon), which are all vulnerable/endangered species pro- tected under the Indian Wildlife Act. |
format |
Article in Journal/Newspaper |
author |
Jayasankar, P Anoop, B Rajagopalan, M |
author_facet |
Jayasankar, P Anoop, B Rajagopalan, M |
author_sort |
Jayasankar, P |
title |
PCR-based sex determination of cetaceans and dugong from the Indian seas |
title_short |
PCR-based sex determination of cetaceans and dugong from the Indian seas |
title_full |
PCR-based sex determination of cetaceans and dugong from the Indian seas |
title_fullStr |
PCR-based sex determination of cetaceans and dugong from the Indian seas |
title_full_unstemmed |
PCR-based sex determination of cetaceans and dugong from the Indian seas |
title_sort |
pcr-based sex determination of cetaceans and dugong from the indian seas |
publisher |
Current Science Association |
publishDate |
2008 |
url |
http://eprints.cmfri.org.in/2/ http://eprints.cmfri.org.in/2/1/1513.pdf http://www.ias.ac.in/currsci/jun102008/1513.pdf |
geographic |
Indian Pacific |
geographic_facet |
Indian Pacific |
genre |
Balaenoptera musculus Blue whale Sperm whale |
genre_facet |
Balaenoptera musculus Blue whale Sperm whale |
op_relation |
http://eprints.cmfri.org.in/2/1/1513.pdf Jayasankar, P and Anoop, B and Rajagopalan, M (2008) PCR-based sex determination of cetaceans and dugong from the Indian seas. Current Science, 94 (11). pp. 1513-1516. |
_version_ |
1766366715758772224 |