Suppression of Dwarf and irregular xylem Phenotypes Generates Low-Acetylated Biomass Lines in Arabidopsis
eskimo1-5 (esk1-5) is a dwarf Arabidopsis (Arabidopsis thaliana) mutant that has a constitutive drought syndrome and collapsed xylem vessels, along with low acetylation levels in xylan and mannan. ESK1 has xylan O-acetyltransferase activity in vitro. We used a suppressor strategy on esk1-5 to screen...
Published in: | Plant Physiology |
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ftclermontuniv:oai:HAL:hal-01204187v1 2024-06-23T07:52:31+00:00 Suppression of Dwarf and irregular xylem Phenotypes Generates Low-Acetylated Biomass Lines in Arabidopsis Bensussan, Matthieu Lefebvre, Valérie Ducamp, Aloise Trouverie, Jacques Gineau, Emilie Vallery-Radot, Marie-Noëlle Guillebaux, Alexia Baldy, Aurélie Naquin, Delphine Herbette, Stéphane Lapierre, Catherine Mouille, Gregory Horlow, Christine Durand-Tardif, Marie-Hélène Institut Jean-Pierre Bourgin (IJPB) Institut National de la Recherche Agronomique (INRA)-AgroParisTech Centre de génétique moléculaire (CGM) Université Paris-Sud - Paris 11 (UP11)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier (PIAF) Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP) Ecophysiologie Végétale, Agronomie et Nutritions (EVA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA) 2015 https://hal.science/hal-01204187 https://doi.org/10.1104/pp.15.00122 en eng HAL CCSD Oxford University Press American Society of Plant Biologists info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.15.00122 info:eu-repo/semantics/altIdentifier/pmid/25888614 hal-01204187 https://hal.science/hal-01204187 doi:10.1104/pp.15.00122 PRODINRA: 307793 PUBMED: 25888614 PUBMEDCENTRAL: PMC4453781 WOS: 000359298200007 ISSN: 0032-0889 EISSN: 1532-2548 Plant Physiology https://hal.science/hal-01204187 Plant Physiology, 2015, 168 (2), pp.452-463. ⟨10.1104/pp.15.00122⟩ [SDV]Life Sciences [q-bio] [SDV.BV]Life Sciences [q-bio]/Vegetal Biology info:eu-repo/semantics/article Journal articles 2015 ftclermontuniv https://doi.org/10.1104/pp.15.00122 2024-06-03T23:59:47Z eskimo1-5 (esk1-5) is a dwarf Arabidopsis (Arabidopsis thaliana) mutant that has a constitutive drought syndrome and collapsed xylem vessels, along with low acetylation levels in xylan and mannan. ESK1 has xylan O-acetyltransferase activity in vitro. We used a suppressor strategy on esk1-5 to screen for variants with wild-type growth and low acetylation levels, a favorable combination for ethanol production. We found a recessive mutation in the KAKTUS (KAK) gene that suppressed dwarfism and the collapsed xylem character, the cause of decreased hydraulic conductivity in the esk1-5 mutant. Backcrosses between esk1-5 and two independent knockout kak mutants confirmed suppression of the esk1-5 effect. kak single mutants showed larger stem diameters than the wild type. The KAK promoter fused with a reporter gene showed activity in the vascular cambium, phloem, and primary xylem in the stem and hypocotyl. However, suppression of the collapsed xylem phenotype in esk1 kak double mutants was not associated with the recovery of cell wall O-acetylation or any major cell wall modifications. Therefore, our results indicate that, in addition to its described activity as a repressor of endoreduplication, KAK may play a role in vascular development. Furthermore, orthologous esk1 kak double mutants may hold promise for ethanol production in crop plants. Article in Journal/Newspaper eskimo* HAL Clermont Auvergne (Université Blaise Pascal Clermont-Ferrand/Université d'Auvergne) Plant Physiology 168 2 452 463 |
institution |
Open Polar |
collection |
HAL Clermont Auvergne (Université Blaise Pascal Clermont-Ferrand/Université d'Auvergne) |
op_collection_id |
ftclermontuniv |
language |
English |
topic |
[SDV]Life Sciences [q-bio] [SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
spellingShingle |
[SDV]Life Sciences [q-bio] [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Bensussan, Matthieu Lefebvre, Valérie Ducamp, Aloise Trouverie, Jacques Gineau, Emilie Vallery-Radot, Marie-Noëlle Guillebaux, Alexia Baldy, Aurélie Naquin, Delphine Herbette, Stéphane Lapierre, Catherine Mouille, Gregory Horlow, Christine Durand-Tardif, Marie-Hélène Suppression of Dwarf and irregular xylem Phenotypes Generates Low-Acetylated Biomass Lines in Arabidopsis |
topic_facet |
[SDV]Life Sciences [q-bio] [SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
description |
eskimo1-5 (esk1-5) is a dwarf Arabidopsis (Arabidopsis thaliana) mutant that has a constitutive drought syndrome and collapsed xylem vessels, along with low acetylation levels in xylan and mannan. ESK1 has xylan O-acetyltransferase activity in vitro. We used a suppressor strategy on esk1-5 to screen for variants with wild-type growth and low acetylation levels, a favorable combination for ethanol production. We found a recessive mutation in the KAKTUS (KAK) gene that suppressed dwarfism and the collapsed xylem character, the cause of decreased hydraulic conductivity in the esk1-5 mutant. Backcrosses between esk1-5 and two independent knockout kak mutants confirmed suppression of the esk1-5 effect. kak single mutants showed larger stem diameters than the wild type. The KAK promoter fused with a reporter gene showed activity in the vascular cambium, phloem, and primary xylem in the stem and hypocotyl. However, suppression of the collapsed xylem phenotype in esk1 kak double mutants was not associated with the recovery of cell wall O-acetylation or any major cell wall modifications. Therefore, our results indicate that, in addition to its described activity as a repressor of endoreduplication, KAK may play a role in vascular development. Furthermore, orthologous esk1 kak double mutants may hold promise for ethanol production in crop plants. |
author2 |
Institut Jean-Pierre Bourgin (IJPB) Institut National de la Recherche Agronomique (INRA)-AgroParisTech Centre de génétique moléculaire (CGM) Université Paris-Sud - Paris 11 (UP11)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier (PIAF) Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP) Ecophysiologie Végétale, Agronomie et Nutritions (EVA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA) |
format |
Article in Journal/Newspaper |
author |
Bensussan, Matthieu Lefebvre, Valérie Ducamp, Aloise Trouverie, Jacques Gineau, Emilie Vallery-Radot, Marie-Noëlle Guillebaux, Alexia Baldy, Aurélie Naquin, Delphine Herbette, Stéphane Lapierre, Catherine Mouille, Gregory Horlow, Christine Durand-Tardif, Marie-Hélène |
author_facet |
Bensussan, Matthieu Lefebvre, Valérie Ducamp, Aloise Trouverie, Jacques Gineau, Emilie Vallery-Radot, Marie-Noëlle Guillebaux, Alexia Baldy, Aurélie Naquin, Delphine Herbette, Stéphane Lapierre, Catherine Mouille, Gregory Horlow, Christine Durand-Tardif, Marie-Hélène |
author_sort |
Bensussan, Matthieu |
title |
Suppression of Dwarf and irregular xylem Phenotypes Generates Low-Acetylated Biomass Lines in Arabidopsis |
title_short |
Suppression of Dwarf and irregular xylem Phenotypes Generates Low-Acetylated Biomass Lines in Arabidopsis |
title_full |
Suppression of Dwarf and irregular xylem Phenotypes Generates Low-Acetylated Biomass Lines in Arabidopsis |
title_fullStr |
Suppression of Dwarf and irregular xylem Phenotypes Generates Low-Acetylated Biomass Lines in Arabidopsis |
title_full_unstemmed |
Suppression of Dwarf and irregular xylem Phenotypes Generates Low-Acetylated Biomass Lines in Arabidopsis |
title_sort |
suppression of dwarf and irregular xylem phenotypes generates low-acetylated biomass lines in arabidopsis |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.science/hal-01204187 https://doi.org/10.1104/pp.15.00122 |
genre |
eskimo* |
genre_facet |
eskimo* |
op_source |
ISSN: 0032-0889 EISSN: 1532-2548 Plant Physiology https://hal.science/hal-01204187 Plant Physiology, 2015, 168 (2), pp.452-463. ⟨10.1104/pp.15.00122⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.15.00122 info:eu-repo/semantics/altIdentifier/pmid/25888614 hal-01204187 https://hal.science/hal-01204187 doi:10.1104/pp.15.00122 PRODINRA: 307793 PUBMED: 25888614 PUBMEDCENTRAL: PMC4453781 WOS: 000359298200007 |
op_doi |
https://doi.org/10.1104/pp.15.00122 |
container_title |
Plant Physiology |
container_volume |
168 |
container_issue |
2 |
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452 |
op_container_end_page |
463 |
_version_ |
1802643840854654976 |