Data from: Environmental stress increases selection against and dominance of deleterious mutations in inbred families of the Pacific oyster Crassostrea gigas
Microsatellite genotypes for Mol. Ecology environmental stress study in C. gigas 5_23_12,The deleterious effects of inbreeding are well documented and of major concern in conservation biology. Stressful environments have generally been shown to increase inbreeding depression; however, little is know...
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ftclemsonuniv:oai:tigerprints.clemson.edu:all_data-3638 2023-06-11T04:11:04+02:00 Data from: Environmental stress increases selection against and dominance of deleterious mutations in inbred families of the Pacific oyster Crassostrea gigas Plough, Louis V. 2012-01-01T08:00:00Z https://tigerprints.clemson.edu/all_data/2638 https://doi.org/10.5061/dryad.376jg en eng Clemson University Libraries https://tigerprints.clemson.edu/all_data/2638 http://datadryad.org/stash/dataset/doi:10.5061/dryad.376jg All Data Sets Experimental genetics deleterious mutations Crassostrea gigas QTL mapping text 2012 ftclemsonuniv https://doi.org/10.5061/dryad.376jg 2023-04-22T22:41:59Z Microsatellite genotypes for Mol. Ecology environmental stress study in C. gigas 5_23_12,The deleterious effects of inbreeding are well documented and of major concern in conservation biology. Stressful environments have generally been shown to increase inbreeding depression; however, little is known about the underlying genetic mechanisms of the inbreeding-by-stress interaction and to what extent the fitness of individual deleterious mutations is altered under stress. Using microsatellite marker segregation data and quantitative trait locus (QTL) mapping methods, I performed a genome scan for deleterious mutations affecting viability (viability or vQTL) in two, inbred families of the Pacific oyster Crassostrea gigas, reared in a stressful, nutrient-poor diet and a favorable, nutrient-rich diet, which had significant effects on growth and survival. Twice as many vQTL were detected in the stressful diet compared with the favorable diet, resulting primarily from substantially greater mortality of homozygous genotypes. At vQTL, estimates of selection (s) and dominance (h) were significantly greater in the stressful environment (s ̅ = 0.86 vs. 0.54 and h ̅ = 0.35 vs. 0.18, in stressful and non-stressful diets, respectively). There was no evidence of interaction between vQTL. Individual vQTL differed across diets in selection only, or in both selection and dominance, and some vQTL were not affected by diet. These results suggest that stress-associated increases in selection against individual deleterious alleles underlie greater inbreeding depression with stress. Furthermore, the finding that inbreeding-by-environment interaction appears, to some extent, to be locus-specific, helps to explain previous observations of lineage-specific expression of inbreeding depression and environment-specific purging, which have important implications for conservation and evolutionary biology. Text Crassostrea gigas Pacific oyster Clemson University: TigerPrints Pacific |
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Clemson University: TigerPrints |
op_collection_id |
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language |
English |
topic |
Experimental genetics deleterious mutations Crassostrea gigas QTL mapping |
spellingShingle |
Experimental genetics deleterious mutations Crassostrea gigas QTL mapping Plough, Louis V. Data from: Environmental stress increases selection against and dominance of deleterious mutations in inbred families of the Pacific oyster Crassostrea gigas |
topic_facet |
Experimental genetics deleterious mutations Crassostrea gigas QTL mapping |
description |
Microsatellite genotypes for Mol. Ecology environmental stress study in C. gigas 5_23_12,The deleterious effects of inbreeding are well documented and of major concern in conservation biology. Stressful environments have generally been shown to increase inbreeding depression; however, little is known about the underlying genetic mechanisms of the inbreeding-by-stress interaction and to what extent the fitness of individual deleterious mutations is altered under stress. Using microsatellite marker segregation data and quantitative trait locus (QTL) mapping methods, I performed a genome scan for deleterious mutations affecting viability (viability or vQTL) in two, inbred families of the Pacific oyster Crassostrea gigas, reared in a stressful, nutrient-poor diet and a favorable, nutrient-rich diet, which had significant effects on growth and survival. Twice as many vQTL were detected in the stressful diet compared with the favorable diet, resulting primarily from substantially greater mortality of homozygous genotypes. At vQTL, estimates of selection (s) and dominance (h) were significantly greater in the stressful environment (s ̅ = 0.86 vs. 0.54 and h ̅ = 0.35 vs. 0.18, in stressful and non-stressful diets, respectively). There was no evidence of interaction between vQTL. Individual vQTL differed across diets in selection only, or in both selection and dominance, and some vQTL were not affected by diet. These results suggest that stress-associated increases in selection against individual deleterious alleles underlie greater inbreeding depression with stress. Furthermore, the finding that inbreeding-by-environment interaction appears, to some extent, to be locus-specific, helps to explain previous observations of lineage-specific expression of inbreeding depression and environment-specific purging, which have important implications for conservation and evolutionary biology. |
format |
Text |
author |
Plough, Louis V. |
author_facet |
Plough, Louis V. |
author_sort |
Plough, Louis V. |
title |
Data from: Environmental stress increases selection against and dominance of deleterious mutations in inbred families of the Pacific oyster Crassostrea gigas |
title_short |
Data from: Environmental stress increases selection against and dominance of deleterious mutations in inbred families of the Pacific oyster Crassostrea gigas |
title_full |
Data from: Environmental stress increases selection against and dominance of deleterious mutations in inbred families of the Pacific oyster Crassostrea gigas |
title_fullStr |
Data from: Environmental stress increases selection against and dominance of deleterious mutations in inbred families of the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Data from: Environmental stress increases selection against and dominance of deleterious mutations in inbred families of the Pacific oyster Crassostrea gigas |
title_sort |
data from: environmental stress increases selection against and dominance of deleterious mutations in inbred families of the pacific oyster crassostrea gigas |
publisher |
Clemson University Libraries |
publishDate |
2012 |
url |
https://tigerprints.clemson.edu/all_data/2638 https://doi.org/10.5061/dryad.376jg |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
All Data Sets |
op_relation |
https://tigerprints.clemson.edu/all_data/2638 http://datadryad.org/stash/dataset/doi:10.5061/dryad.376jg |
op_doi |
https://doi.org/10.5061/dryad.376jg |
_version_ |
1768385900392218624 |