1H–NMR Metabolomic Biomarkers of Poor Outcome after
Background: Hemorrhagic shock (HS) following trauma is a leading cause of death among persons under the age of 40. During HS the body undergoes systemic warm ischemia followed by reperfusion during medical intervention. Ischemia/ reperfusion (I/R) results in a disruption of cellular metabolic proces...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.691.371 2023-05-15T15:08:28+02:00 1H–NMR Metabolomic Biomarkers of Poor Outcome after Lori K. Bogren Carl J. Murphy Erin L. Johnston Neeraj Sinha Natalie J. Serkova Kelly L. Drew The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.691.371 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161479/pdf/pone.0107493.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.691.371 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161479/pdf/pone.0107493.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161479/pdf/pone.0107493.pdf text ftciteseerx 2016-01-08T18:24:57Z Background: Hemorrhagic shock (HS) following trauma is a leading cause of death among persons under the age of 40. During HS the body undergoes systemic warm ischemia followed by reperfusion during medical intervention. Ischemia/ reperfusion (I/R) results in a disruption of cellular metabolic processes that ultimately lead to tissue and organ dysfunction or failure. Resistance to I/R injury is a characteristic of hibernating mammals. The present study sought to identify circulating metabolites in the rat as biomarkers for metabolic alterations associated with poor outcome after HS. Arctic ground squirrels (AGS), a hibernating species that resists I/R injury independent of decreased body temperature (warm I/R), was used as a negative control. Methodology/principal findings: Male Sprague-Dawley rats and AGS were subject to HS by withdrawing blood to a mean arterial pressure (MAP) of 35 mmHg and maintaining the low MAP for 20 min before reperfusing with Ringers. The animals’ temperature was maintained at 3760.5uC for the duration of the experiment. Plasma samples were taken immediately before hemorrhage and three hours after reperfusion. Hydrophilic and lipid metabolites from plasma were then analyzed via 1H–NMR from unprocessed plasma and lipid extracts, respectively. Rats, susceptible to I/R injury, had a qualitative shift in their hydrophilic metabolic fingerprint including differential activation of glucose and anaerobic metabolism and had alterations in several metabolites during I/R indicative of metabolic adjustments and organ damage. In contrast, I/R injury Text Arctic Unknown Arctic |
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Background: Hemorrhagic shock (HS) following trauma is a leading cause of death among persons under the age of 40. During HS the body undergoes systemic warm ischemia followed by reperfusion during medical intervention. Ischemia/ reperfusion (I/R) results in a disruption of cellular metabolic processes that ultimately lead to tissue and organ dysfunction or failure. Resistance to I/R injury is a characteristic of hibernating mammals. The present study sought to identify circulating metabolites in the rat as biomarkers for metabolic alterations associated with poor outcome after HS. Arctic ground squirrels (AGS), a hibernating species that resists I/R injury independent of decreased body temperature (warm I/R), was used as a negative control. Methodology/principal findings: Male Sprague-Dawley rats and AGS were subject to HS by withdrawing blood to a mean arterial pressure (MAP) of 35 mmHg and maintaining the low MAP for 20 min before reperfusing with Ringers. The animals’ temperature was maintained at 3760.5uC for the duration of the experiment. Plasma samples were taken immediately before hemorrhage and three hours after reperfusion. Hydrophilic and lipid metabolites from plasma were then analyzed via 1H–NMR from unprocessed plasma and lipid extracts, respectively. Rats, susceptible to I/R injury, had a qualitative shift in their hydrophilic metabolic fingerprint including differential activation of glucose and anaerobic metabolism and had alterations in several metabolites during I/R indicative of metabolic adjustments and organ damage. In contrast, I/R injury |
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The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Lori K. Bogren Carl J. Murphy Erin L. Johnston Neeraj Sinha Natalie J. Serkova Kelly L. Drew |
spellingShingle |
Lori K. Bogren Carl J. Murphy Erin L. Johnston Neeraj Sinha Natalie J. Serkova Kelly L. Drew 1H–NMR Metabolomic Biomarkers of Poor Outcome after |
author_facet |
Lori K. Bogren Carl J. Murphy Erin L. Johnston Neeraj Sinha Natalie J. Serkova Kelly L. Drew |
author_sort |
Lori K. Bogren |
title |
1H–NMR Metabolomic Biomarkers of Poor Outcome after |
title_short |
1H–NMR Metabolomic Biomarkers of Poor Outcome after |
title_full |
1H–NMR Metabolomic Biomarkers of Poor Outcome after |
title_fullStr |
1H–NMR Metabolomic Biomarkers of Poor Outcome after |
title_full_unstemmed |
1H–NMR Metabolomic Biomarkers of Poor Outcome after |
title_sort |
1h–nmr metabolomic biomarkers of poor outcome after |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.691.371 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161479/pdf/pone.0107493.pdf |
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Arctic |
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Arctic |
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Arctic |
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Arctic |
op_source |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161479/pdf/pone.0107493.pdf |
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http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.691.371 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161479/pdf/pone.0107493.pdf |
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