Tropical Biomedicine 31(3): 422–431 (2014) Comparison between Quantitative Buffy Coat (QBC) and Giemsa-stained Thin Film (GTF) technique for blood
Abstract. The quantitative buffy coat (QBC) technique and conventional Giemsa thin blood smear was compared to determine the sensitivity and specificity of the technique in detecting blood parasitic infection of the rodent populations from four urban cities in Peninsular Malaysia. A total of 432 blo...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.674.8124 2023-05-15T18:05:18+02:00 Tropical Biomedicine 31(3): 422–431 (2014) Comparison between Quantitative Buffy Coat (QBC) and Giemsa-stained Thin Film (GTF) technique for blood The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.674.8124 http://www.msptm.org/files/422_-_431_Mohd_Zain_SN.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.674.8124 http://www.msptm.org/files/422_-_431_Mohd_Zain_SN.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.msptm.org/files/422_-_431_Mohd_Zain_SN.pdf text ftciteseerx 2016-01-08T17:32:56Z Abstract. The quantitative buffy coat (QBC) technique and conventional Giemsa thin blood smear was compared to determine the sensitivity and specificity of the technique in detecting blood parasitic infection of the rodent populations from four urban cities in Peninsular Malaysia. A total of 432 blood samples from four rat species (Rattus norvegicus, Rattus rattus diardii, Rattus exulans and Rattus argentiventer) were screened using both techniques and successfully detected two blood protozoan species (Trypanosoma lewisi and Plasmodium sp.) with Trypanosoma lewisi predominantly infecting the population. Results showed that Giemsa-stained thin film (GTF) was the better detection method on blood parasitemia (46.7%) compared to Quantitative Buffy Coat method (38.9%) with overall detection technique sensitivity and specificity at 83.2 % and 74.8 % respectively. The sensitivity in detection of Trypanosoma lewisi was 84.4 % with value slightly lower for Plasmodium sp. infections at 76.6%. Statistical analysis proved that GTF technique was significantly more sensitive in the detection of blood protozoan infections in the rodent population compared to QBC (p<0.05). Text Rattus rattus Unknown |
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Abstract. The quantitative buffy coat (QBC) technique and conventional Giemsa thin blood smear was compared to determine the sensitivity and specificity of the technique in detecting blood parasitic infection of the rodent populations from four urban cities in Peninsular Malaysia. A total of 432 blood samples from four rat species (Rattus norvegicus, Rattus rattus diardii, Rattus exulans and Rattus argentiventer) were screened using both techniques and successfully detected two blood protozoan species (Trypanosoma lewisi and Plasmodium sp.) with Trypanosoma lewisi predominantly infecting the population. Results showed that Giemsa-stained thin film (GTF) was the better detection method on blood parasitemia (46.7%) compared to Quantitative Buffy Coat method (38.9%) with overall detection technique sensitivity and specificity at 83.2 % and 74.8 % respectively. The sensitivity in detection of Trypanosoma lewisi was 84.4 % with value slightly lower for Plasmodium sp. infections at 76.6%. Statistical analysis proved that GTF technique was significantly more sensitive in the detection of blood protozoan infections in the rodent population compared to QBC (p<0.05). |
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The Pennsylvania State University CiteSeerX Archives |
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Text |
title |
Tropical Biomedicine 31(3): 422–431 (2014) Comparison between Quantitative Buffy Coat (QBC) and Giemsa-stained Thin Film (GTF) technique for blood |
spellingShingle |
Tropical Biomedicine 31(3): 422–431 (2014) Comparison between Quantitative Buffy Coat (QBC) and Giemsa-stained Thin Film (GTF) technique for blood |
title_short |
Tropical Biomedicine 31(3): 422–431 (2014) Comparison between Quantitative Buffy Coat (QBC) and Giemsa-stained Thin Film (GTF) technique for blood |
title_full |
Tropical Biomedicine 31(3): 422–431 (2014) Comparison between Quantitative Buffy Coat (QBC) and Giemsa-stained Thin Film (GTF) technique for blood |
title_fullStr |
Tropical Biomedicine 31(3): 422–431 (2014) Comparison between Quantitative Buffy Coat (QBC) and Giemsa-stained Thin Film (GTF) technique for blood |
title_full_unstemmed |
Tropical Biomedicine 31(3): 422–431 (2014) Comparison between Quantitative Buffy Coat (QBC) and Giemsa-stained Thin Film (GTF) technique for blood |
title_sort |
tropical biomedicine 31(3): 422–431 (2014) comparison between quantitative buffy coat (qbc) and giemsa-stained thin film (gtf) technique for blood |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.674.8124 http://www.msptm.org/files/422_-_431_Mohd_Zain_SN.pdf |
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Rattus rattus |
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Rattus rattus |
op_source |
http://www.msptm.org/files/422_-_431_Mohd_Zain_SN.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.674.8124 http://www.msptm.org/files/422_-_431_Mohd_Zain_SN.pdf |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766176769811939328 |