Analysis of Fungal Flora in Indoor Dust by
In recent years increasing attention has been given to the potential health effects of fungal exposure in indoor environments. We used large-scale sequencing of the fungal internal transcribed spacer region (ITS) of nuclear ribosomal DNA to describe the mycoflora of two office buildings over the fou...
Other Authors: | |
---|---|
Format: | Text |
Language: | English |
Published: |
2007
|
Subjects: | |
Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.665.1037 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223223/pdf/0692-07.pdf |
id |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.665.1037 |
---|---|
record_format |
openpolar |
spelling |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.665.1037 2023-05-15T15:59:35+02:00 Analysis of Fungal Flora in Indoor Dust by The Pennsylvania State University CiteSeerX Archives 2007 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.665.1037 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223223/pdf/0692-07.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.665.1037 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223223/pdf/0692-07.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223223/pdf/0692-07.pdf text 2007 ftciteseerx 2016-01-08T17:03:29Z In recent years increasing attention has been given to the potential health effects of fungal exposure in indoor environments. We used large-scale sequencing of the fungal internal transcribed spacer region (ITS) of nuclear ribosomal DNA to describe the mycoflora of two office buildings over the four seasons. DNA sequencing was complemented by cultivation, ergosterol determination, and quantitative PCR analyses. Sequences of 1,339 clones were clustered into 394 nonredundant fungal operational taxonomical units containing sequences from 18 fungal subclasses. The observed flora differed markedly from that recovered by cultivation, the major differences being the near absence of several typical indoor mold genera such as Penicillium and Aspergillus spp. and a high prevalence of basidiomycetes in clone libraries. A total of 55 % of the total diversity constituted of unidentifiable ITS sequences, some of which may represent novel fungal species. Dominant species were Cladosporium cladosporioides and C. herbarum, Cryptococcus victoriae, Leptosphaerulina americana and L. char-tarum, Aureobasidium pullulans, Thekopsora areolata, Phaeococcomyces nigricans, Macrophoma sp., and several Malassezia species. Seasonal differences were observed for community composition, with ascomycetous molds and basidiomycetous yeasts predominating in the winter and spring and Agaricomycetidae basidiomycetes predominating in the fall. The comparison of methods suggested that the cloning, cultivation, and quantitative PCR methods complemented each other, generating a more comprehensive picture of fungal flora than any of Text Cryptococcus victoriae Unknown |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftciteseerx |
language |
English |
description |
In recent years increasing attention has been given to the potential health effects of fungal exposure in indoor environments. We used large-scale sequencing of the fungal internal transcribed spacer region (ITS) of nuclear ribosomal DNA to describe the mycoflora of two office buildings over the four seasons. DNA sequencing was complemented by cultivation, ergosterol determination, and quantitative PCR analyses. Sequences of 1,339 clones were clustered into 394 nonredundant fungal operational taxonomical units containing sequences from 18 fungal subclasses. The observed flora differed markedly from that recovered by cultivation, the major differences being the near absence of several typical indoor mold genera such as Penicillium and Aspergillus spp. and a high prevalence of basidiomycetes in clone libraries. A total of 55 % of the total diversity constituted of unidentifiable ITS sequences, some of which may represent novel fungal species. Dominant species were Cladosporium cladosporioides and C. herbarum, Cryptococcus victoriae, Leptosphaerulina americana and L. char-tarum, Aureobasidium pullulans, Thekopsora areolata, Phaeococcomyces nigricans, Macrophoma sp., and several Malassezia species. Seasonal differences were observed for community composition, with ascomycetous molds and basidiomycetous yeasts predominating in the winter and spring and Agaricomycetidae basidiomycetes predominating in the fall. The comparison of methods suggested that the cloning, cultivation, and quantitative PCR methods complemented each other, generating a more comprehensive picture of fungal flora than any of |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
title |
Analysis of Fungal Flora in Indoor Dust by |
spellingShingle |
Analysis of Fungal Flora in Indoor Dust by |
title_short |
Analysis of Fungal Flora in Indoor Dust by |
title_full |
Analysis of Fungal Flora in Indoor Dust by |
title_fullStr |
Analysis of Fungal Flora in Indoor Dust by |
title_full_unstemmed |
Analysis of Fungal Flora in Indoor Dust by |
title_sort |
analysis of fungal flora in indoor dust by |
publishDate |
2007 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.665.1037 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223223/pdf/0692-07.pdf |
genre |
Cryptococcus victoriae |
genre_facet |
Cryptococcus victoriae |
op_source |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223223/pdf/0692-07.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.665.1037 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2223223/pdf/0692-07.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766395521617887232 |