Anaesthetization of Arctic charr Salvelinus alpinus (L.) with tricaine methanesulphonate or 2-phenoxyethanol for immediate blood sampling

• Main Authors: J. S. Bystriansky, P. J. Leblanc, J. S. Ballantyne
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Published: 2006
• Subjects: Arctic; Arctic charr; Salvelinus alpinus
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.658.818; http://comparativephys.ca/files/uploaded_media/publications/22802.pdf

Anaesthetization of Arctic charr Salvelinus alpinus with tricaine methanesulphonate or 2-phenoxyethanol did not alter plasma protein, glucose, free amino acid, non-esterified fatty acid, ion or osmolality levels, suggesting that their use allows for the normal determination of these plasma variables. # 2006 The Fisheries Society of the British Isles Key words: amino acid; anaesthetic; glucose; non-esterified fatty acid; 2-phenoxyethanol; tricaine methanesulphonate. The measurement of circulating blood metabolites is useful for monitoring the physiological and biochemical status of fishes. The stress associated with hand-ling and blood sampling, however, can quickly alter the levels of some blood constituents. For example, stress has been shown to increase the levels of blood cortisol (Laidley & Leatherland, 1988), glucose (Vijayan & Moon, 1994), cate-cholamine and corticosteroid (Mazeaud et al., 1977). Changes in the circulating levels of these metabolites can have significant effects on fish metabolism. Anaesthetics have been employed to increase the ease of handling fishes and to reduce the effects of stress during blood sampling. Two anaesthetics