2005. Effect of incubation temperature on carbohydrate digestion in important teleosts for aquaculture

• Main Authors: Eustratios S Papoutsoglou, Alastair R Lyndon
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: a amylase; a glucosidase; capacity; car- bohydrases; digestion; enzymes; ¢sh; Tive; Salmo salar
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.654.4388; http://www.uesc.br/cursos/pos_graduacao/mestrado/animal/bibliografia2008/papoutsoglou_e_lyndon_2005.pdf

The activity and capacity (activity tissue weight) of digestive carbohydrases (total carbohydrase, a-glucosidase, a-amylase) was examined in vitro under a range of (more realistic) incubation temperatures (5, 18 and 25 1C) and pH (7.6) more likely to be en-countered during rearing, along the digestive tract of important teleost species for aquaculture (Dicen-trarchus labrax, Sparus aurata, Oreochromis aureus, Oncorhynchus mykiss and Salmo salar). Results indi-cate, among other things, an overestimation of diges-tive carbohydrase levels when performing assays at 37 1C, a di¡erent e¡ect of temperature on digestive enzyme performance along the digestive tract of examined species, and the increased importance of a-glucosidase towards carbohydrate digestion. Im-plications regarding the capacity of each species for carbohydrate digestion in nature, as well as feed manufacturing, are discussed.