Review Establishment of a UK National Influenza H5 Laboratory Network
Avian (H5N1) influenza continues to pose a significant threat to human health, although it remains a zoonotic infection. Sensitive and robust surveillance measures are required to detect any evidence that the virus has acquired the ability to transmit between humans and emerge as the next pandemic s...
Main Authors: | , , , |
---|---|
Other Authors: | |
Format: | Text |
Language: | English |
Published: |
2007
|
Subjects: | |
Online Access: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.627.9199 http://jmm.sgmjournals.org/content/56/10/1263.full.pdf |
id |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.627.9199 |
---|---|
record_format |
openpolar |
spelling |
ftciteseerx:oai:CiteSeerX.psu:10.1.1.627.9199 2023-05-15T15:34:28+02:00 Review Establishment of a UK National Influenza H5 Laboratory Network Martin D. Curran Joanna S. Ellis Tim G. Wreghitt Maria C. Zambon The Pennsylvania State University CiteSeerX Archives 2007 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.627.9199 http://jmm.sgmjournals.org/content/56/10/1263.full.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.627.9199 http://jmm.sgmjournals.org/content/56/10/1263.full.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://jmm.sgmjournals.org/content/56/10/1263.full.pdf text 2007 ftciteseerx 2016-01-08T15:18:47Z Avian (H5N1) influenza continues to pose a significant threat to human health, although it remains a zoonotic infection. Sensitive and robust surveillance measures are required to detect any evidence that the virus has acquired the ability to transmit between humans and emerge as the next pandemic strain. An integral part of the pandemic planning response in the UK was the creation in 2005 of the UK National H5 Laboratory Network, capable of rapidly and accurately identifying potential human H5N1 infections in all regions of the UK, and the Republic of Ireland. This review details the challenges that designing molecular detection methods for a rapidly evolving virus present, and the strategic decisions and choices required to ensure successful establishment of a functional national laboratory network, providing round the clock testing for H5N1. Laboratory partnerships have delivered improved real-time one-step multiplex PCR methodologies to ensure streamlined testing capable of not only detecting H5 but also a differential diagnosis of seasonal influenza A/B. A range of fully validated real-time PCR H5 confirmatory assays have been developed to run in parallel with a universal first-screening assay. Regular proficiency panels together with weekly surveillance runs, intermittent on-call testing for suspect cases of avian flu in returning travellers, and several outbreaks of avian influenza Text Avian flu Unknown |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftciteseerx |
language |
English |
description |
Avian (H5N1) influenza continues to pose a significant threat to human health, although it remains a zoonotic infection. Sensitive and robust surveillance measures are required to detect any evidence that the virus has acquired the ability to transmit between humans and emerge as the next pandemic strain. An integral part of the pandemic planning response in the UK was the creation in 2005 of the UK National H5 Laboratory Network, capable of rapidly and accurately identifying potential human H5N1 infections in all regions of the UK, and the Republic of Ireland. This review details the challenges that designing molecular detection methods for a rapidly evolving virus present, and the strategic decisions and choices required to ensure successful establishment of a functional national laboratory network, providing round the clock testing for H5N1. Laboratory partnerships have delivered improved real-time one-step multiplex PCR methodologies to ensure streamlined testing capable of not only detecting H5 but also a differential diagnosis of seasonal influenza A/B. A range of fully validated real-time PCR H5 confirmatory assays have been developed to run in parallel with a universal first-screening assay. Regular proficiency panels together with weekly surveillance runs, intermittent on-call testing for suspect cases of avian flu in returning travellers, and several outbreaks of avian influenza |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Martin D. Curran Joanna S. Ellis Tim G. Wreghitt Maria C. Zambon |
spellingShingle |
Martin D. Curran Joanna S. Ellis Tim G. Wreghitt Maria C. Zambon Review Establishment of a UK National Influenza H5 Laboratory Network |
author_facet |
Martin D. Curran Joanna S. Ellis Tim G. Wreghitt Maria C. Zambon |
author_sort |
Martin D. Curran |
title |
Review Establishment of a UK National Influenza H5 Laboratory Network |
title_short |
Review Establishment of a UK National Influenza H5 Laboratory Network |
title_full |
Review Establishment of a UK National Influenza H5 Laboratory Network |
title_fullStr |
Review Establishment of a UK National Influenza H5 Laboratory Network |
title_full_unstemmed |
Review Establishment of a UK National Influenza H5 Laboratory Network |
title_sort |
review establishment of a uk national influenza h5 laboratory network |
publishDate |
2007 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.627.9199 http://jmm.sgmjournals.org/content/56/10/1263.full.pdf |
genre |
Avian flu |
genre_facet |
Avian flu |
op_source |
http://jmm.sgmjournals.org/content/56/10/1263.full.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.627.9199 http://jmm.sgmjournals.org/content/56/10/1263.full.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766364853287518208 |