H (2003) Effects of cytochalasin B on fertilization and ploidy in the Pacific oyster Crassostrea gigas
The effects of cytochalasin B on fertilization and ploidy in Crassostrea gigas were examined in a controlled experiment. This enabled us to better understand why variable ploidy results have been found with the retention ofpolar bodies in previous studies on bivalve mollusks, and what the interactio...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.627.835 2023-05-15T15:58:02+02:00 H (2003) Effects of cytochalasin B on fertilization and ploidy in the Pacific oyster Crassostrea gigas Christophe Ledu Helen Mccomble The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.627.835 http://archimer.ifremer.fr/doc/2003/publication-2845.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.627.835 http://archimer.ifremer.fr/doc/2003/publication-2845.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://archimer.ifremer.fr/doc/2003/publication-2845.pdf Key words Polyploidy polar body cytochalasin B fertilization Crassostreagigas text ftciteseerx 2016-01-08T15:18:32Z The effects of cytochalasin B on fertilization and ploidy in Crassostrea gigas were examined in a controlled experiment. This enabled us to better understand why variable ploidy results have been found with the retention ofpolar bodies in previous studies on bivalve mollusks, and what the interaction with fertilization may be. By looking at the early development of Crassas/rea gigas embryos with sequential sampies stained using Hoechst 33258, a fluorescent DNA-specific dye, we compared normal evolution of fertilized eggs with that obtained with cytochalasin B treatrnent. In treated eggs, embryos with both three and four pronuclei were found after the second meiotic division. When embryos had three pronuclei, these were identified as the female pronucleus plus two chromatin groups arising from the retention of the two polar bodies. The fourth pronucleus present in sorne embryos was the male pronucleus. The absence of the male pronucleus in the embryos with only three pronuc1ei was interpreted as gynogenetic development due to the inhibitory effects of cytochalasin B on penetration of the sperm into the eggs. This result explains the findings of several previous studies where different ploidies resulted from double polar body inh ibition in bivalve species. Implications ofsuch treatments for post-embryo genic development are discussed. Text Crassostrea gigas Pacific oyster Unknown Pacific |
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Unknown |
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ftciteseerx |
language |
English |
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Key words Polyploidy polar body cytochalasin B fertilization Crassostreagigas |
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Key words Polyploidy polar body cytochalasin B fertilization Crassostreagigas Christophe Ledu Helen Mccomble H (2003) Effects of cytochalasin B on fertilization and ploidy in the Pacific oyster Crassostrea gigas |
topic_facet |
Key words Polyploidy polar body cytochalasin B fertilization Crassostreagigas |
description |
The effects of cytochalasin B on fertilization and ploidy in Crassostrea gigas were examined in a controlled experiment. This enabled us to better understand why variable ploidy results have been found with the retention ofpolar bodies in previous studies on bivalve mollusks, and what the interaction with fertilization may be. By looking at the early development of Crassas/rea gigas embryos with sequential sampies stained using Hoechst 33258, a fluorescent DNA-specific dye, we compared normal evolution of fertilized eggs with that obtained with cytochalasin B treatrnent. In treated eggs, embryos with both three and four pronuclei were found after the second meiotic division. When embryos had three pronuclei, these were identified as the female pronucleus plus two chromatin groups arising from the retention of the two polar bodies. The fourth pronucleus present in sorne embryos was the male pronucleus. The absence of the male pronucleus in the embryos with only three pronuc1ei was interpreted as gynogenetic development due to the inhibitory effects of cytochalasin B on penetration of the sperm into the eggs. This result explains the findings of several previous studies where different ploidies resulted from double polar body inh ibition in bivalve species. Implications ofsuch treatments for post-embryo genic development are discussed. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Christophe Ledu Helen Mccomble |
author_facet |
Christophe Ledu Helen Mccomble |
author_sort |
Christophe Ledu |
title |
H (2003) Effects of cytochalasin B on fertilization and ploidy in the Pacific oyster Crassostrea gigas |
title_short |
H (2003) Effects of cytochalasin B on fertilization and ploidy in the Pacific oyster Crassostrea gigas |
title_full |
H (2003) Effects of cytochalasin B on fertilization and ploidy in the Pacific oyster Crassostrea gigas |
title_fullStr |
H (2003) Effects of cytochalasin B on fertilization and ploidy in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
H (2003) Effects of cytochalasin B on fertilization and ploidy in the Pacific oyster Crassostrea gigas |
title_sort |
h (2003) effects of cytochalasin b on fertilization and ploidy in the pacific oyster crassostrea gigas |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.627.835 http://archimer.ifremer.fr/doc/2003/publication-2845.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
http://archimer.ifremer.fr/doc/2003/publication-2845.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.627.835 http://archimer.ifremer.fr/doc/2003/publication-2845.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766393757909909504 |