Psychrophilic Enzymes from the Antarctic Isol

• Main Authors: Rashidah A. R, Sabrina S, Ainihayati A. R, Shanmugapriya P, Razip S
• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: Antarctic; The Antarctic; Antarc*
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.627.740; http://eprints.usm.my/4832/1/Psychrophilic_Enzymes_From_The_Antartic_Isolates.pdf

In the project, the Antarctic fresh samples rvill be obtained from diffelent sources. An obligate psychrophilic micloorganism froru tl-re fresh sarlple wili be isolated by the growth and temperature studies. The selected Antarctic microotganistns u,ill be identified by conrrentional morphological studies, biochemicai tests and moiecttlar characterisation based on the nucleotides sequence in genes 265 rDNA, t 65 rRNA aud Intet'ual Transcribed Spacer (lTS). The potential industrial enzyrnes (eg. lipase and protease) and fundarnental metabolic (eg. glycolytic pathway) enzymes from the isolated rnicroolgauisn will be screened for the enzyme stuclies. Cloning of interest genes rvill be carried out. The recoubinant ptoteins will be further expressed and purified for the strucftrral studies that u,ill involve protein clystallisation and X-ray crystallography diffraction analysis for the detetmination of protein strlrctul'e. 1 1.