A molecular epidemiological study of rabies virus in central Ontario and western
Rabies persists in Ontario wildlife in two predominant species: the red fox (Vulpes vulpes) and the striped skunk (Mephitis mephitis). A protocol applying reverse transcription/polymerase chain reaction (RT/PCR) and restriction endonuclease analysis (REA) to the rabies virus nucleoprotein gene was p...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.621.4159 2023-05-15T14:56:05+02:00 A molecular epidemiological study of rabies virus in central Ontario and western Susan A. Nadin-davis Er I. W The Pennsylvania State University CiteSeerX Archives 1994 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.621.4159 http://vir.sgmjournals.org/content/75/10/2575.full.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.621.4159 http://vir.sgmjournals.org/content/75/10/2575.full.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://vir.sgmjournals.org/content/75/10/2575.full.pdf text 1994 ftciteseerx 2016-01-08T15:01:32Z Rabies persists in Ontario wildlife in two predominant species: the red fox (Vulpes vulpes) and the striped skunk (Mephitis mephitis). A protocol applying reverse transcription/polymerase chain reaction (RT/PCR) and restriction endonuclease analysis (REA) to the rabies virus nucleoprotein gene was previously reported by Nadin-Davis et al. (Journal of General Virology 74, 829-837, 1993) to be useful for discrimination of rabies virus variants in Ontario. Four main types, which showed no host species specificity but which did exhibit different geographical distributions, were identified. Between 1989 and 1992 an area north and west of the city of North Bay experienced unusual and substantial rabies activity. In this report we describe the use of these molecular techniques to investigate the epidemiology of this recent rabies outbreak in central Ontario. It is shown that two of the four previously identified variants had invaded this region from the south and east, but in addition viruses very closely related to arctic isolates of rabies virus were found. The nucleoprotein and gly-coprotein genes of this arctic type were sequenced and compared to those of its more southerly neighbours. Text Arctic Unknown Arctic North Bay ENVELOPE(-37.690,-37.690,-54.040,-54.040) |
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ftciteseerx |
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English |
description |
Rabies persists in Ontario wildlife in two predominant species: the red fox (Vulpes vulpes) and the striped skunk (Mephitis mephitis). A protocol applying reverse transcription/polymerase chain reaction (RT/PCR) and restriction endonuclease analysis (REA) to the rabies virus nucleoprotein gene was previously reported by Nadin-Davis et al. (Journal of General Virology 74, 829-837, 1993) to be useful for discrimination of rabies virus variants in Ontario. Four main types, which showed no host species specificity but which did exhibit different geographical distributions, were identified. Between 1989 and 1992 an area north and west of the city of North Bay experienced unusual and substantial rabies activity. In this report we describe the use of these molecular techniques to investigate the epidemiology of this recent rabies outbreak in central Ontario. It is shown that two of the four previously identified variants had invaded this region from the south and east, but in addition viruses very closely related to arctic isolates of rabies virus were found. The nucleoprotein and gly-coprotein genes of this arctic type were sequenced and compared to those of its more southerly neighbours. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Susan A. Nadin-davis Er I. W |
spellingShingle |
Susan A. Nadin-davis Er I. W A molecular epidemiological study of rabies virus in central Ontario and western |
author_facet |
Susan A. Nadin-davis Er I. W |
author_sort |
Susan A. Nadin-davis |
title |
A molecular epidemiological study of rabies virus in central Ontario and western |
title_short |
A molecular epidemiological study of rabies virus in central Ontario and western |
title_full |
A molecular epidemiological study of rabies virus in central Ontario and western |
title_fullStr |
A molecular epidemiological study of rabies virus in central Ontario and western |
title_full_unstemmed |
A molecular epidemiological study of rabies virus in central Ontario and western |
title_sort |
molecular epidemiological study of rabies virus in central ontario and western |
publishDate |
1994 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.621.4159 http://vir.sgmjournals.org/content/75/10/2575.full.pdf |
long_lat |
ENVELOPE(-37.690,-37.690,-54.040,-54.040) |
geographic |
Arctic North Bay |
geographic_facet |
Arctic North Bay |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
http://vir.sgmjournals.org/content/75/10/2575.full.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.621.4159 http://vir.sgmjournals.org/content/75/10/2575.full.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766328125872930816 |