North American Journal of Fisheries Management 23:1108–1113, 2003 American Fisheries Society 2003 Producing Fluorescent Marks on Atlantic Salmon Fin Rays and Scales with Calcein via Osmotic Induction
Abstract.—Calcified tissues of Atlantic salmon in both larval and postscale developmental life stages became labeled with the fluorochrome dye known as calcein in 7 min by sequential im-mersion in salt and calcein solutions. I introduce the term ‘‘osmotic induction’ ’ to describe this process. At 47...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.618.9860 2023-05-15T15:30:51+02:00 North American Journal of Fisheries Management 23:1108–1113, 2003 American Fisheries Society 2003 Producing Fluorescent Marks on Atlantic Salmon Fin Rays and Scales with Calcein via Osmotic Induction Jerre W. Mohler The Pennsylvania State University CiteSeerX Archives 1108 application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.618.9860 http://www.fws.gov/northeast/fisherycenter/pdfs/mohler2003.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.618.9860 http://www.fws.gov/northeast/fisherycenter/pdfs/mohler2003.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.fws.gov/northeast/fisherycenter/pdfs/mohler2003.pdf text 1108 ftciteseerx 2016-01-08T14:52:55Z Abstract.—Calcified tissues of Atlantic salmon in both larval and postscale developmental life stages became labeled with the fluorochrome dye known as calcein in 7 min by sequential im-mersion in salt and calcein solutions. I introduce the term ‘‘osmotic induction’ ’ to describe this process. At 47 d posttreatment, fish that had been exposed to osmotic induction of calcein as nonfeeding larvae exhibited mortality and growth equivalent to that of nonexposed fish (P, 0.05). Atlantic salmon parr (at 5 months posthatch) exposed to osmotic induction of calcein exhibited no mortality during a 120-d posttreatment period. In addition to inducing a calcein mark on fin rays of all larvae and parr, the procedure also produced a distinct calcein mark on scales of parr. A second exposure of parr to osmotic induction 90 d after the first produced a conspicuous 2-band pattern on scales which corresponded to the two separate marking events. Calcein marks are invisible to the unaided eye but were observed without sacrificing the experimental fish by em-ploying a calcein detection device (patent applied for) and fluorescence microscopy. Osmotic induction of calcein marks is a potentially useful technique for batch-marking fish because it greatly reduces the amount of contact time between the fish and marking solution and produces an easily identified mark; this may enable salmon fishery managers and researchers to perform a Text Atlantic salmon Unknown |
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English |
description |
Abstract.—Calcified tissues of Atlantic salmon in both larval and postscale developmental life stages became labeled with the fluorochrome dye known as calcein in 7 min by sequential im-mersion in salt and calcein solutions. I introduce the term ‘‘osmotic induction’ ’ to describe this process. At 47 d posttreatment, fish that had been exposed to osmotic induction of calcein as nonfeeding larvae exhibited mortality and growth equivalent to that of nonexposed fish (P, 0.05). Atlantic salmon parr (at 5 months posthatch) exposed to osmotic induction of calcein exhibited no mortality during a 120-d posttreatment period. In addition to inducing a calcein mark on fin rays of all larvae and parr, the procedure also produced a distinct calcein mark on scales of parr. A second exposure of parr to osmotic induction 90 d after the first produced a conspicuous 2-band pattern on scales which corresponded to the two separate marking events. Calcein marks are invisible to the unaided eye but were observed without sacrificing the experimental fish by em-ploying a calcein detection device (patent applied for) and fluorescence microscopy. Osmotic induction of calcein marks is a potentially useful technique for batch-marking fish because it greatly reduces the amount of contact time between the fish and marking solution and produces an easily identified mark; this may enable salmon fishery managers and researchers to perform a |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Jerre W. Mohler |
spellingShingle |
Jerre W. Mohler North American Journal of Fisheries Management 23:1108–1113, 2003 American Fisheries Society 2003 Producing Fluorescent Marks on Atlantic Salmon Fin Rays and Scales with Calcein via Osmotic Induction |
author_facet |
Jerre W. Mohler |
author_sort |
Jerre W. Mohler |
title |
North American Journal of Fisheries Management 23:1108–1113, 2003 American Fisheries Society 2003 Producing Fluorescent Marks on Atlantic Salmon Fin Rays and Scales with Calcein via Osmotic Induction |
title_short |
North American Journal of Fisheries Management 23:1108–1113, 2003 American Fisheries Society 2003 Producing Fluorescent Marks on Atlantic Salmon Fin Rays and Scales with Calcein via Osmotic Induction |
title_full |
North American Journal of Fisheries Management 23:1108–1113, 2003 American Fisheries Society 2003 Producing Fluorescent Marks on Atlantic Salmon Fin Rays and Scales with Calcein via Osmotic Induction |
title_fullStr |
North American Journal of Fisheries Management 23:1108–1113, 2003 American Fisheries Society 2003 Producing Fluorescent Marks on Atlantic Salmon Fin Rays and Scales with Calcein via Osmotic Induction |
title_full_unstemmed |
North American Journal of Fisheries Management 23:1108–1113, 2003 American Fisheries Society 2003 Producing Fluorescent Marks on Atlantic Salmon Fin Rays and Scales with Calcein via Osmotic Induction |
title_sort |
north american journal of fisheries management 23:1108–1113, 2003 american fisheries society 2003 producing fluorescent marks on atlantic salmon fin rays and scales with calcein via osmotic induction |
publishDate |
1108 |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.618.9860 http://www.fws.gov/northeast/fisherycenter/pdfs/mohler2003.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
http://www.fws.gov/northeast/fisherycenter/pdfs/mohler2003.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.618.9860 http://www.fws.gov/northeast/fisherycenter/pdfs/mohler2003.pdf |
op_rights |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766361315425648640 |