Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human
The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongy-loidiasis. In addition, the WB results were compared to the enzyme-linked immuno...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.615.4915 2023-05-15T18:05:28+02:00 Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human Luciana Pereira Silva Ivanildes Solange Costa Barcelos Andréia Barcelos Passos Foued Salmen Espindola Dulcinéa Maria Barbosa Campos Julia Maria Costa-cruz The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.615.4915 en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.615.4915 Metadata may be used without restrictions as long as the oai identifier remains attached to it. https://tspace.library.utoronto.ca/bitstream/1807/8122/1/oc03141.pdf text ftciteseerx 2016-01-08T14:43:25Z The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongy-loidiasis. In addition, the WB results were compared to the enzyme-linked immunosorbent assay (ELISA) and the indirect immunofluorescence antibody test (IFAT) results. Serum samples of 180 individuals were analyzed (80 with strongyloidiasis, 60 with other intestinal parasitoses, and 40 healthy individuals). S. ratti was obtained from fecal culture of experimentally infected Rattus rattus. For IFAT, S. ratti larvae were used as antigen and S. ratti larval antigenic extracts were employed in WB and ELISA. Eleven S. ratti antigenic components were predominantly recognized by IgG antibodies in sera of patients with strongyloidiasis. There was a positive concordance for the three tests in 87.5 % of the cases of strongyloidiasis. The negative concordance in the three tests was 94 % and 97.5%, in patients with other intestinal parasitoses and healthy individuals, respectively. In cases of positive ELISA and negative IFAT results, diagnosis could be confirmed by WB. ELISA, IFAT, and WB using S. ratti antigens showed a high rate of sensitivity and specificity. In conclusion, WB using S. ratti larval extract was able to recognize 11 immunodominant antigenic components, showing to be a useful tool to define the diagnosis in cases of equivocal serology. Key words: Strongyloides stercoralis- Strongyloides ratti- strongyloidiasis- immunodiagnosis- Western blotting Text Rattus rattus Unknown |
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English |
description |
The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongy-loidiasis. In addition, the WB results were compared to the enzyme-linked immunosorbent assay (ELISA) and the indirect immunofluorescence antibody test (IFAT) results. Serum samples of 180 individuals were analyzed (80 with strongyloidiasis, 60 with other intestinal parasitoses, and 40 healthy individuals). S. ratti was obtained from fecal culture of experimentally infected Rattus rattus. For IFAT, S. ratti larvae were used as antigen and S. ratti larval antigenic extracts were employed in WB and ELISA. Eleven S. ratti antigenic components were predominantly recognized by IgG antibodies in sera of patients with strongyloidiasis. There was a positive concordance for the three tests in 87.5 % of the cases of strongyloidiasis. The negative concordance in the three tests was 94 % and 97.5%, in patients with other intestinal parasitoses and healthy individuals, respectively. In cases of positive ELISA and negative IFAT results, diagnosis could be confirmed by WB. ELISA, IFAT, and WB using S. ratti antigens showed a high rate of sensitivity and specificity. In conclusion, WB using S. ratti larval extract was able to recognize 11 immunodominant antigenic components, showing to be a useful tool to define the diagnosis in cases of equivocal serology. Key words: Strongyloides stercoralis- Strongyloides ratti- strongyloidiasis- immunodiagnosis- Western blotting |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Luciana Pereira Silva Ivanildes Solange Costa Barcelos Andréia Barcelos Passos Foued Salmen Espindola Dulcinéa Maria Barbosa Campos Julia Maria Costa-cruz |
spellingShingle |
Luciana Pereira Silva Ivanildes Solange Costa Barcelos Andréia Barcelos Passos Foued Salmen Espindola Dulcinéa Maria Barbosa Campos Julia Maria Costa-cruz Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human |
author_facet |
Luciana Pereira Silva Ivanildes Solange Costa Barcelos Andréia Barcelos Passos Foued Salmen Espindola Dulcinéa Maria Barbosa Campos Julia Maria Costa-cruz |
author_sort |
Luciana Pereira Silva |
title |
Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human |
title_short |
Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human |
title_full |
Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human |
title_fullStr |
Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human |
title_full_unstemmed |
Western Blotting Using Strongyloides ratti Antigen for the Detection of IgG Antibodies as Confirmatory Test in Human |
title_sort |
western blotting using strongyloides ratti antigen for the detection of igg antibodies as confirmatory test in human |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.615.4915 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
https://tspace.library.utoronto.ca/bitstream/1807/8122/1/oc03141.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.615.4915 |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
_version_ |
1766176945044717568 |