Alternative cell line for the isolation of salmonid alphavirus-1 TARANGANI HERATH
Salmonid alphavirus (SAV) has recently become an economically important pathogen in salmonid aqua-culture in Europe. Subtype SAV-1 causes salmon pancreas disease (SPD) in Atlantic salmon in Scotland and Ireland, and was first isolated on Chinook salmon embryo-214 (CHSE-214) cells in 1995 in Ireland;...
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ftciteseerx:oai:CiteSeerX.psu:10.1.1.614.5086 2023-05-15T15:31:47+02:00 Alternative cell line for the isolation of salmonid alphavirus-1 TARANGANI HERATH Janina Costa Kim Thompson Alexandra Adams The Pennsylvania State University CiteSeerX Archives application/pdf http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.614.5086 http://www.landbunadur.is/landbunadur/wgsamvef.nsf/8bbba2777ac88c4000256a89000a2ddb/93e7759f88de1e9400257672004b7e4d/$file/ias alternative cell line.pdf en eng http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.614.5086 http://www.landbunadur.is/landbunadur/wgsamvef.nsf/8bbba2777ac88c4000256a89000a2ddb/93e7759f88de1e9400257672004b7e4d/$file/ias alternative cell line.pdf Metadata may be used without restrictions as long as the oai identifier remains attached to it. http://www.landbunadur.is/landbunadur/wgsamvef.nsf/8bbba2777ac88c4000256a89000a2ddb/93e7759f88de1e9400257672004b7e4d/$file/ias alternative cell line.pdf YFIRLIT text ftciteseerx 2016-01-08T14:40:45Z Salmonid alphavirus (SAV) has recently become an economically important pathogen in salmonid aqua-culture in Europe. Subtype SAV-1 causes salmon pancreas disease (SPD) in Atlantic salmon in Scotland and Ireland, and was first isolated on Chinook salmon embryo-214 (CHSE-214) cells in 1995 in Ireland; several established cell lines have since been tested for viral growth, although the ability of these cell lines to support primary virus isolation has not being examined. In the present study, CHSE-214, Chum salmon heart-1 (CHH-1) and Salmon head kidney-1 (SHK-1) cell lines were evaluated for isolation of SAV-1 from kidney samples of experimentally infected Atlantic salmon (Salmo salar). The presence of infection in these samples was confirmed both by cell culture and reverse transcription polymerase chain reaction (RT-PCR). Homogenates of kidney from fish 3 days post-infection (p.i.) were inoculated onto the three cell lines and the development of a cytopathic effect (CPE) recorded. The CHH-1 cells produced a rapid CPE from Day 6 p.i., while the CHSE-214 cells showed the presence of a CPE from Day 10 p.i. In comparison, a CPE developed much later in the SHK-1 cells, from Day 20 p.i. The virus was successfully isolated on all three cell lines in subsequent passages, indicating that CHSE-214, CHH-1, and SHK-1 cells can be used for the isolation and culture of SAV-1. The CHH-1 cell line, however, has proven the most useful, since the CPE developed the quickest in this cell line. Text Atlantic salmon Salmo salar Unknown Sav’ ENVELOPE(156.400,156.400,68.817,68.817) |
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YFIRLIT Janina Costa Kim Thompson Alexandra Adams Alternative cell line for the isolation of salmonid alphavirus-1 TARANGANI HERATH |
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YFIRLIT |
description |
Salmonid alphavirus (SAV) has recently become an economically important pathogen in salmonid aqua-culture in Europe. Subtype SAV-1 causes salmon pancreas disease (SPD) in Atlantic salmon in Scotland and Ireland, and was first isolated on Chinook salmon embryo-214 (CHSE-214) cells in 1995 in Ireland; several established cell lines have since been tested for viral growth, although the ability of these cell lines to support primary virus isolation has not being examined. In the present study, CHSE-214, Chum salmon heart-1 (CHH-1) and Salmon head kidney-1 (SHK-1) cell lines were evaluated for isolation of SAV-1 from kidney samples of experimentally infected Atlantic salmon (Salmo salar). The presence of infection in these samples was confirmed both by cell culture and reverse transcription polymerase chain reaction (RT-PCR). Homogenates of kidney from fish 3 days post-infection (p.i.) were inoculated onto the three cell lines and the development of a cytopathic effect (CPE) recorded. The CHH-1 cells produced a rapid CPE from Day 6 p.i., while the CHSE-214 cells showed the presence of a CPE from Day 10 p.i. In comparison, a CPE developed much later in the SHK-1 cells, from Day 20 p.i. The virus was successfully isolated on all three cell lines in subsequent passages, indicating that CHSE-214, CHH-1, and SHK-1 cells can be used for the isolation and culture of SAV-1. The CHH-1 cell line, however, has proven the most useful, since the CPE developed the quickest in this cell line. |
author2 |
The Pennsylvania State University CiteSeerX Archives |
format |
Text |
author |
Janina Costa Kim Thompson Alexandra Adams |
author_facet |
Janina Costa Kim Thompson Alexandra Adams |
author_sort |
Janina Costa |
title |
Alternative cell line for the isolation of salmonid alphavirus-1 TARANGANI HERATH |
title_short |
Alternative cell line for the isolation of salmonid alphavirus-1 TARANGANI HERATH |
title_full |
Alternative cell line for the isolation of salmonid alphavirus-1 TARANGANI HERATH |
title_fullStr |
Alternative cell line for the isolation of salmonid alphavirus-1 TARANGANI HERATH |
title_full_unstemmed |
Alternative cell line for the isolation of salmonid alphavirus-1 TARANGANI HERATH |
title_sort |
alternative cell line for the isolation of salmonid alphavirus-1 tarangani herath |
url |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.614.5086 http://www.landbunadur.is/landbunadur/wgsamvef.nsf/8bbba2777ac88c4000256a89000a2ddb/93e7759f88de1e9400257672004b7e4d/$file/ias alternative cell line.pdf |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
http://www.landbunadur.is/landbunadur/wgsamvef.nsf/8bbba2777ac88c4000256a89000a2ddb/93e7759f88de1e9400257672004b7e4d/$file/ias alternative cell line.pdf |
op_relation |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.614.5086 http://www.landbunadur.is/landbunadur/wgsamvef.nsf/8bbba2777ac88c4000256a89000a2ddb/93e7759f88de1e9400257672004b7e4d/$file/ias alternative cell line.pdf |
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Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
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1766362299645296640 |