• Other Authors: The Pennsylvania State University CiteSeerX Archives
• Format: Text
• Language: English
• Subjects: Antarc*; Antarctica
• Online Access: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.606.1569; http://zernike.eldoc.ub.rug.nl/FILES/root/2009/ReacFunctPolMiletic/2009ReacFunctPolymMiletic.pdf

Enzyme immobilization friendly conditions [1,2]. However, most of the natural enzymes show none of their profound characteristics in organic solvents and can easily denature under industrial conditions (high temper-ature, mechanical shear, etc.). Recovery of enzymes from reaction solutions and separation of the enzymes from substrates and prod-ucts are generally difficult. Therefore, there have been many stud-ies to stabilize enzyme activity and increase operational stability. provides: (i) increased enzyme activity; (ii) increased enantio-selectivity, (iii) temperature stability and (iv) easy recovery from the reaction medium for their reuse. On the other hand, immobi-lized enzymes are protected by solid matrix that limits their con-formational variations diminishing unpredictable changes of their characteristic properties. Solid matrix also overshadows enzymes from temperature changes, pH alternation and shaking conditions. Candida antarctica lipase B is a versatile enzyme for enantio-and regio-selective transformations on many low molar mass and polymer substrates and has been found to possess a broad range